Table of Content

  

10 March 2015
Volume 53 Issue 3

Expressions of MiR210 and stat3 following global ischemia in rats and the potential relationship among MiR210, stat3 and HIF-1α

FAN Shaohua, ZHANG Yichao, YUAN Xiaodong, BI Jingwen, ZHANG Yichen, LI Yun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  1-5.  doi:10.6040/j.issn.1671-7554.0.2014.943

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Objective To study the expression of signal transducer and activator of transcription 3 (stat3) in hippocampus and the expression of MiR210 in cerebral cortex following global ischemia in rats, and to explore the potential relationship among MiR210, stat3 and hypoxia inducible factor-1α (HIF-1α). Methods Sixty-six SD rats were randomly divided into three groups:sham-operated group, global ischemia group (GI group), and stattic+global ischemia group (stattic+GI group). The global ischemia model was induced by modified four-vessel occlusion method. Stat3, p-stat3 and HIF-1α protein expressions in hippocampus were detected by Western blotting. MiR210 and HIF-1α mRNA expressions in cerebral cortex were detected by Real-time PCR. Results Compared with sham-operated group, stat3, p-stat3 and HIF-1α protein expressions in hippocampus and MiR210 and HIF-1α mRNA expressions in cerebral cortex of GI group significantly increased (all P<0.05). Compared with GI group, p-stat3 and HIF-1α protein expressions in hippocampus of stattic+ GI group significantly decreased, stat3 protein expression showed no statistical difference (P>0.05), and HIF-1α mRNA expression in cerebral cortex signifcantly decreased (P<0.05). Conclusion Expressions of MiR210 and stat3 have a marketable change after global ischemia reperfusion, and regulatory relationships may exist among stat3, MiR210 and HIF-1α.

Effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer and the underlying mechanisms

YANG Lu, LIU Yanguo, LI Jisheng, WANG Xiuwen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  6-11.  doi:10.6040/j.issn.1671-7554.0.2014.934

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Objective To investigate the effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer (NSCLC) A549 and H1975 cells and explore the underlying molecular mechanisms. Methods A549 and H1975 cells were treated with various concentrations of genistein, cisplatin and their combination respectively. CCK-8 assay and flow cytometric analysis were used to investigate their effect on cell proliferation and apoptosis; Western blotting analysis was used to investigate the expressions of nuclear factor-kappa B(NF-κB) and its downstream molecule c-myc. Results Bufalin significantly inhibited the proliferation and induced apoptosis of A549 and H1975 cells in a time-and dose-dependent manner. Low concentration of bufalin significantly increased chemosensitivity to cisplatin in A549 and H1975 cells, accompanied by down-regulation of NF-κB and its downstream molecule c-myc. Conclusion Bufalin significantly increased chemosensitivity to cisplatin in A549 and H1975 cells, which is partly through inhibiting the activation of NF-κB and down-regulating its downstream molecular c-myc.

Label-free quantification of proteomic changes in ethanol-induced hepatocyte

WANG Xiaoyue, WANG Daoguang, GUO Jisheng, LI Cuiling, JING Ruirui, LÜ Xin, CHEN Xinjun, YANG Jinghua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  12-16.  doi:10.6040/j.issn.1671-7554.0.2014.766

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Objective To explore the proteomic changes in ethanol-induced hepatocyte cell line by a label-free quantification method. Methods Hepatocellular carcinoma cell line HepG2 was treated with ethanol and underwent in-solution-digestion. The total peptides were detected by liquid chromatography-mass spectrometry (LC-MS) and analyzed by Progenesis LC-MS, a commercial label-free quantification software. The differentially expressed proteins were further counted by Panther 9.0 and String 9.1 databases. Results More than 309 proteins were defined with significant difference in amount by Progenesis LC-MS. They were belong to catalytic enzymes, cell-signaling-related proteins, structural proteins, and soon which were involved in a variety of intracellular physiological responses, such as apoptosis and immune response. String showed there was a close relationship among these proteins. Conclusion Label-free quantification is an effective way of researching the mechanism of ethanol-induced hepatocyte damage.

Expression of human papillomavirus late capsid protein 1 in CaSki cells

DU Yijiang, XIAO Changyi, YE Hong, TANG Guicheng, LI Jiahua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  17-21.  doi:10.6040/j.issn.1671-7554.0.2014.779

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Objective To investigate the expression of human papil1omavirus (HPV) late capsid protein 1 (L1) in CaSki cells. Methods Using polyvalent HPV L1 mice monoclonal antibody as the probe, the L1 protein expression in CaSki cells was investigated by ELISA, immunohistochemistry and Western blotting. L1 mRNA expression was detected by RT-PCR. Results HPV L1 was strong positive in CaSki cells, indicating that L1 protein was expressed in CaSki cells. L1 mRNA was expressed in CaSki cells. Conclusion CaSki cells integrated HPV 16 DNA genome can express L1 protein.

Mass spectrometry analysis of differentially expressed proteins between HPV immortalized cell line and SiHa cell line

LIU Yafei, ZHANG Qishu, YUAN Shoudao, Jason J. Chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  22-26.  doi:10.6040/j.issn.1671-7554.0.2014.800

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Objective To explore the mechanism by which human papilloma virus (HPV) induces cervical cancer and to identify proteins differentially expressed between HPV immortalized cells and SiHa cells. Methods Differentially expressed proteins between HPV immortalized cells and SiHa cells were identified by high performance liquid chromatography and mass spectrometry, and analyzed by label-free quantity software and bioinformatics approaches. Results A total of 10 691 proteins were identified and 6 342 succinylation sites were found; label-free quantification analyses indicated that 572 proteins were differentially expressed between the two types of cells; bioinfomatic analysis showed that 273 proteins in HPV immortalized cells were mostly associated with disease pathway, while 299 proteins in cervical cancer cells were mostly involved in metabolic and protein-produced pathway. Although succinylation modifications had no differences, a new succinylation site at SGK1 K41 was found. Conclusion Compared with HPV immortalized cells, SiHa cells demonstrate higher level of metabolism and stronger protein-synthesis, while most up-regulated proteins in HPV immortalized cell are associated with particular diseases.

Enhancement of tendon-bone healing by topical use of simvastatin after ACL reconstruction

LIN Jinxiu, SUN Dongsheng, ZHENG Xiao, YIN Dechao, LI Ye, ZHANG Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  27-31.  doi:10.6040/j.issn.1671-7554.0.2014.887

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Objective To evaluate the enhancement potential of topical use of simvastatin for tendon-bone healing. Methods A total of 15 rabbits were enrolled to establish anterior cruciate ligament (ACL) reconstruction models. The tibial side was screw fixed while the femoral side was suspending fixed in both knees of the rabbits. Simvastatin was inserted into the left knee (experimental side) bone tunnel with a gelatin sponge carrier, while gelatin sponge was inserted into the right knee (control side) bone tunnel. Three rabbits were sacrificed in week 2, 4 and 8 postoperatively. Tendon-bone interface was histologically assessed. Six rabbits were sacrificed for biomechanical tests in week 8. Results Histological analysis in week 2 demonstrated that tendon-bone healing was significantly greater with angiogenesis in the simvastatin-treated side than in the control side. In week 4, a great quantity of sharpey fibers at tendon-bone interface were observed in the experimental side, yet there were no sharpey fibers in the control side. In week 8, tendon-bone interface was narrow and unclear in the experimental side, yet a few sharpey fibers were observed in the control side. The average load to failure in the experimental side was significantly greater than in the control side. Conclusion Topical use of simvastatin promotes the tendon-bone healing after ACL reconstruction.

Effects of PTH on gene ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP during the distraction osteogenesis in rat mandible

DU Tengfei, LIU Guoming, GUO Xiaomei, Ching-Chang Ko, SHANG Hongguo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  32-35.  doi:10.6040/j.issn.1671-7554.0.2014.590

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Objective To explore the effects of parathyroid hormone (PTH) on gene ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP during the distraction osteogenesis (DO) in rat mandible. Methods A total of 30 DO models were established with male rats, which were then randomly divided into 5 groups, with 6 animals in each group. Group 1(DO, no PTH) was harvested 8 weeks after surgery, and the tissues were tested using HE and micro-CT. Group 2 (DO, no PTH), Group 3(DO, PTH), Group 4 (no DO, PTH), Group 5 (control Group, no DO, no PTH) were harvested 1 week after surgery, and Real-time PCR was used to quantify the genes:ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP. Results In Group 1, histological test and micro-CT showed new bone formation in the gap. In Groups 2, 3 and 4, especially in Group 3, RT-PCR test showed that compared with the control group, OPN, COL1, RUNX2 and ALP genes were significantly increased (P<0.05), while ONC , C-FOS, and VEGF genes were not significantly different (P>0.05). Conclusion Intermittent administration of PTH increases bone formation only in the distraction period. PTH has synergetic effect on gene expressions of OPN, COL1, RUNX2 and ALP.

Expression of Hsp27 in lipopolysaccharide-induced injury of human gingival fibroblasts

ZHU Zhenkun, ZHOU Qin, SUN Ruinan, XU Xin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  36-40.  doi:10.6040/j.issn.1671-7554.0.2014.909

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Objective To observe the expression of heat shock protein 27 (Hsp27) in injury of human gingival fibroblasts (HGFs) induced by lipopolysaccharide (LPS) with different concentrations. Methods The 3^rd-8^th generations of cultured HGFs were treated with serially diluted LPS. Would healing assay was used to determine the migration of HGFs and Western blotting was used to determine the expression of Hsp27 in them. Results The migration capability of HGFs was weakened with the increase of LPS concentration. Expression of Hsp27 was negative in normal HGFs, but was positive in the LPS-intervened HGFs with a concentration-dependent manner. Conclusion Hsp27 concentration increases in LPS-induced injury of HGFs, which suggests that Hsp27 plays an important role during the occurrence of LPS-induced injury of HGFs.

Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines

GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  41-45.  doi:10.6040/j.issn.1671-7554.0.2014.618

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Objective To investigate the expression of the cutaneous T-cell lymphoma-associated antigen 5(cTAGE5) in tumor cell lines and its biological role in the development of tumor preliminarily. Methods Expression of cTAGE5 in tumor cell lines was detected by immunofluorescence. Ovarian clear cell adenocarcinoma cell line ES-2, which was proved to be positive expression of cTAGE5, were divided into normal control group, liposome control group, siRNA transfection group and epirubicin control group(final concentration 1×10^-6mol/L). The Proliferation and growth ability of ES-2 were detected by plate clone formation test and MTT test respectively, and migration ability was detected by cell scratch assay. Results cTAGE5 was expressed in cell lines got from human breast cancer, lung cancer and ovarian cancer, but little in cell lines from human stomach and liver cancer. Compared with normal control group, growth and proliferation ability of tumor cells in siRNA transfection group decreased(P<0.01), migration ability reduced (P<0.01), and proliferation ability of tumor cells in epirubicin control group decreased(P<0.01), also migration ability reduced (P<0.01). Conclusion Expression of cTAGE5 in tumor cell lines from different tissue was different. Inhibition of cTAGE5 expression can depress the proliferation, growth and migration ability of tumor cells.

Effects of Flos Lonicerae Japonicae extract on pruritus induced by compound 48/80, histamine and substance P in ICR mice

BAI Xue, HUANG Huifeng, GUAN Yudong, CHI Lianli

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  46-49.  doi:10.6040/j.issn.1671-7554.0.2014.951

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Objective To study the effect of Flos Lonicerae Japonicae on pruritus induced by various pruritic agents including compound 48/80, histamine and substance P in mice. Methods Flos Lonicerae Japonicae extracts(0.3 g/kg, 0.8 g/kg and 2.4 g/kg) were respectively administered orally to ICR mice 1 h before the injection of compound 48/80, histamine and substance P. Immediately after the injection of pruritogens, the animals were put into the acrylic cage again, and their behaviors were videotaped. Results Flos Lonicerae Japonicae dose-dependently inhibited substance P-induced scratching. However, no inhibitory effect was observed on the scratching behavior induced by histamine or compound 48/80. Conclusion Flos Lonicerae Japonicae shows significant antipruritic effect on substance P-induced pruritus in ICR mice. The primary site of its antipruritic activity is epidermal keratinocytes rather than mast cells.

Proliferative effect of TGF-β1 phage model peptide on fibroblasts

LIU Zhenzhong, JIANG Duyin, WANG Wei, ZONG Xianlei, ZHANG Jixun, LIU Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  50-55.  doi:10.6040/j.issn.1671-7554.0.2014.690

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Objective To isolate transforming growth factor-beta1 (TGF-β1) phage model peptides from phage display 12-mer peptide library, and to evaluate their proliferative effect on normal skin fibroblasts. Methods Phage 12-mer display peptide library was screened with monoclonal anti-human TGF-β1 to get specific phage model peptides. The model with sequence most similar to TGF-β1 was chosen and 4 groups were enrolled:negative control group, MP13 control group, TGF-β1 control group, and phage model peptide group. MTT assay was used for the quantitative determination of cellular proliferation. Immunofluorescence assay was employed to show the affinity of the model peptide on skin fibroblasts. Quantitative Real-time PCR analysis was carried out to detect the expressions of I collagen (COL1), III collagen (COL3) and connective tissue growth factor (CTGF). Results A total of 10 phage model peptides were obtained. The first one with sequence most similar to TGF-β1 was chosen. The MTT results showed that the phage model peptide could promote fibroblast proliferation (P<0.05). Immunofluorescence assay revealed that the model peptide on phages rather than phages could bind to the fibroblasts (P<0.05). The Real-time PCR analysis suggested that the relative expressions of COL1 and COL3 significantly increased in the model group than in the negative control group and M13 group (P<0.05). The expression of CTGF mRNA increased dramatically after treatment withphage model peptide, and reached the peak at 2 d (P<0.05). Conclusion The phage model peptide isolated from phage 12-mer display peptide library can promote the proliferation of normal skin fibroblasts by regulating the expression of CTGF.

Changes of Runx2 and Osterix expression in type 1 diabetic rats and the intervention effect of zoledronic acid

ZHAO Xu, CUI Min, YU Lingzhi, ZHANG Na, CAO Luning

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  56-61.  doi:10.6040/j.issn.1671-7554.0.2014.744

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Objective To observe the levels of serum insulin and osteocalcin, osteoblast specific transcription factor Runx2 and Osterix (OSX) gene in type 1 diabetic rats, and the intervention effect of zoledronic acid. Methods A total of 170 Wistar rats were randomly divided into two groups:40 rats were enrolled into the control group the other animals were injected with streptozotocin (60 mg/kg) to establish type 1 diabetic models. After that, 120 rats in this group were randomly divided into the model group (n=40), prevention group (n=40) and treatment group (n=40). Rats in the prevention group and treatment group received a bolus injection of zoledronic acid (0.1 mg/kg) at the onset of diabetes and 2 weeks later, respectively. The serum INS and OC were detected with ELISA. Expressions of Runx2 and OSX mRNA in the right femur were detected with RT-PCR. Results ELISA results showed that the serum INS in the model group, prevention group and treatment group was significantly reduced compared with that in the control group (P<0.05). The serum OC was lower in the model group that in the control group (P<0.05), while higher in the prevention group than in the control group and model group (P<0.05). At week 12, serum OC was higher in the treatment group than in the prevention group (P<0.05). RT-PCR tests showed that the expressions of Runx2 andOSX mRNA were significantly lower in the model group than in the control group (P<0.05), while remarkably higher in the prevention group and treatment group than in the control group and model group (P<0.05). The upregulation was higher in the prevention group than in the model group. Conclusion Expressions of Runx2 and OSX mRNA were significantly decreased in the bone of type 1 diabetic rat models. Prophylactic use of zoledronic acid can improve the levels of serum OC, and reverse the decreasing expression of Runx2 and OSX mRNA, thus promoting bone formation.

Effect of different circumferential pulmonary vein ablation endpoints on prognosis of atrial fibrillation patients:bidirectional block between pulmonary veins and left atrium

LI Man, ZHONG Jingquan, YUE Xin, ZHU Qing, ZHENG Zhaotong, RONG Bing, YI Shaolei, XIE Fei, ZHANG Yun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  62-68.  doi:10.6040/j.issn.1671-7554.0.2014.029

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Objective To study the effect of different endpoints of ablation on the prognosis of atrial fibrillation (AF) patients. Methods A total of 137 patients with symptomatic AF (113 paroxysmal AF and 24 persistent AF) underwent 3D mapping. Radiofrequency energy was released in one encircling line to the endpoint of bidirectional conduction block between the pulmonary veins (PVs) and the left atrium (LA). After the postoperative observation with 30 minutes, isolation was successful in 110 AF patients (92 paroxysmal AF and 18 persistent AF). The 92 paroxysmal AF patients were divided into two groups:one group included 36 patients in which all PVPs disappeared, and the other group included 56 patients in which at least one of pulmonary vein potentials (PVPs) delayed by more than 30 ms. Results During two years follow-up, the recurrence of paroxysmal AF had no significant difference between the two groups (P=0.159). Conclusion Bidirectional block has no obvious effect on the living quality and recurrence of paroxysmal atrial fibrillation patients compared with those in which PVPs disappeared.

Neuroendoscopic repair of traumatic cerebrospinal fluid rhinorrhea

WANG Chunlai, XU Shujun, WANG Hongliang, SHANG Jingrui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  69-72.  doi:10.6040/j.issn.1671-7554.0.2014.895

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Objective To explore the surgical procedure and efficacy of transnasal neuroendoscopic repair of traumatic cerebrospinal fluid rhinorrhea (CSFR). Methods A retrospective analysis was performed on the clinical data of 22 cases with traumatic CSFR repaired by neuroendoscopy from December 2010 to December 2013. The diagnosis, location of rhinorrhea fistulas, methods of the operation, materials for repair and clinical efficacy were discussed. Results CSFR was repaired successfully during the first attempt in 21 of 22 cases, The twenty-one cases were follwed up for 8 to 24 months postoperatively, and none of them had recurrence during this period. One case, who developed intracranial infection and acute obstructive hydrocephalus, gave up treatment and denoted as the failure case. Conclusion Transnasal neuroendoscopic repair of traumatic CSFR should be the first choice of operative method for traumatic CSFR because of its minimal invasion and high successful rate.

Expressions and significances of HER2 and p53 in different evolution stages of gastric adenocarcinoma

JIA Zhenjun, QU Yong, ZHANG Shijie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  73-76.  doi:10.6040/j.issn.1671-7554.1.2014.012

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Objective To evaluate the expressions of HER2 and p53 in chronic gastritis, precancerous lesions and gastric adenocarcinomas and to explore their roles in gastric adenocarcinomas. Methods The expressions of HER2 and p53 in chronic gastritis(102 cases), gastric dysplasia (158 cases, including 102 cases of low grade dysplasia and 56 cases of high grade dysplasia) and gastric adenocarcinomas(152 cases) were determined with SP immunohistochemical staining. The relationship and clinicopathological significance were analyzed. Results Immunohistochemical results showed that no positive expressions of HER2 and p53 were found in the tissues of chronic gastritis. The positive expression rates of HER2 in gastric low grade dysplasia, high grade dysplasia and gastric adenocarcinomas were 3.92%,28.57% and 44.09%, and those of p53 were 0%, 33.93% and 50.66% respectively. The expressions of HER2 and p53 gradually increased in low grade dysplasia, high grade dysplasia and adenocarcinoma (P<0.001). Positive correlations between HER2 and p53 were found in both high grade dysplasia and adenocarcinoma(r=0.465 and 0.505 respectively, P<0.001). Conclusion HER2 and p53 play important roles in gastric carcinogenesis.

Clinical value of laparoscopic ultrasonography in assisting laparoscopic partial nephrectomy for centrally located para-hilus renal tumors

REN Juchao, LI Mingbao, LIU Huanyin, FANG Zhiqing, YAN Lei, XU Zhonghua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  77-80.  doi:10.6040/j.issn.1671-7554.0.2014.930

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Objective To evaluate the applicative value of intra-operative laparoscopic ultrasonography ( LUS) in laparoscopic partial nephrectomy (LPN) of centrally located para-hilus renal tumors. Methods Clinical date of 4 patients who underwent LPN for centrally located renal tumors with LUS in our hospital during March to July of 2014 were retrospectively analyzed. Two of the patients were operated through abdominal cavity and 2 were operated through retroperitoneum. Indicators such as operation time, blood loss, pathology, incisal margin and so on were assessed. Results LUS showed that malignant renal tumors displayed irregular shape, clear boundary and inhomogeneous echo. Color Doppler ultrasonography showed blood flow within or around the tumors, which provided more information than normal ultrasonography before operation and helped to determine the scope of the renal tumor resection. All cases were confirmed renal cell carcinoma and negative margin with postoperative pathology. Conclusion It is of high clinical value to use LUS in LPN for centrally located renal tumors with real-time image, which helps surgeons to determine the location of the tumor and the position relation between the tumor and vessels and the collection system. At the same time, it also helps to reduce intra-operative hemorrhage, provide negative incisal margin and guarantee the safety of operation.

Clinical characteristics of prostatic cystadenoma

JIANG Xuewen, CHEN Jun, ZHANG Zhaocun, ZHU Yaofeng, MENG Hui, SHI Benkang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  81-86.  doi:10.6040/j.issn.1671-7554.0.2014.442

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Objective To explore the clinical characteristics, diagnosis and treatment of prostatic cystadenoma. Methods Papers published during 1988 to 2014 in databases such as PubMed, MEDLINE, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI) and Wangfang Data were retrieved with queries including prostatic cystadenoma, PC, neoplasm, clinical symptoms, prognosis, diagnosis, treatment and imagelogy. Results A total of 22 foreign and 8 domestic papers were obtained, the age of patients ranged from 15 to 80 years and the main symptom was dysuria. Most patients had higher or normal prostate specific antigen value. The results of CT scan and MRI showed that there were multilocular cystic changes. Various kinds of treatment were adopted, among which surgical removal could cure this disease. Conclusion For patients with prostatic cystadenoma, preoperative transperineal puncture can effectively avoid abscess formation.

Gene detection and analysis for hemophilia B among populations in Shandong Province

LU Min, CHEN Yun, DING Botong, CHANG Yali, ZHOU Yawei, ZHAO Aiping, GUO Nongjian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  87-92.  doi:10.6040/j.issn.1671-7554.0.2014.844

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Objective To analyze the FIX gene mutation type of hemophilia B among populations in Shandong Province, and to explore the molecular mechanism of this disease. Methods The 8 exons of FIX gene and their flanking sequence of 24 cases of hemophilia B from 19 different families were detected with PCR amplification and direct sequencing. The data obtained were compared with data in the international FIX gene mutations database. Results A total of 18 different types of mutations were detected. Our study first discovered 5 new mutation types, including c.200delA, c.306delT, c.688-690delGGA, c.1025C > G, and c.1157C >A. Conclusion FIX gene mutation is the fundamental cause of hemophilia B, which presents high heterogeneity and dispersibility. This experiment has discovered 5 kinds of new mutations, which not only enrich the FIX gene mutations spectrum, but also provide the molecular basis of the pathogenesis.

Clinical study on 151 cases of orbital tumors in the elderly

SONG Xian, XU Xiaoyi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(3):  93-96.  doi:10.6040/j.issn.1671-7554.0.2014.925

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Objective To analyze the clinical characteristics, pathological classification, surgical approaches and therapeutic effect of orbital tumors in elderly patients. Methods Data of 151 cases of surgical treatment of orbital tumors in patients aged ≥ 60 years at Qilu Hospital during Jan. to Mar. 2014 were retrospectively reviewed. Results The clinical features included proptosis, restricted eye movements, impaired vision and so on. Imaging tests could help to identify the location and size of tumors, and select surgical methods. The surgical approaches included anterior orbitotomy in 97 cases, lateral orbitotomy in 39 cases, medial orbitotomy in 10 cases, and exenteration in 5 cases. Of all tumors, 103 (68.21%) were benign and 48 (31.79%) were malignant. Among the 103 benign cases, the top 3 were cavernous hemangioma, lacrimal gland pleomorphic adenomas, and idiopathic orbital inflammation. Among the 48 malignant cases, the top 3 were malignant lymphoma, adenoid cystic carcinoma and pleomorphic adenocarcinoma. Conclusion The elderly should take radiological examinations once proptosis appears. Orbital surgery is the preferred way of treatment, which should take into consideration the location and size of tumors and tolerance of patients. Malignant lymphoma is the most common malignant orbital tumor in the elderly.