JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.943

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Expressions of MiR210 and stat3 following global ischemia in rats and the potential relationship among MiR210, stat3 and HIF-1α

FAN Shaohua1, ZHANG Yichao2, YUAN Xiaodong2, BI Jingwen1, ZHANG Yichen2, LI Yun2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Critical Care Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-12-11 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To study the expression of signal transducer and activator of transcription 3 (stat3) in hippocampus and the expression of MiR210 in cerebral cortex following global ischemia in rats, and to explore the potential relationship among MiR210, stat3 and hypoxia inducible factor-1α (HIF-1α). Methods Sixty-six SD rats were randomly divided into three groups:sham-operated group, global ischemia group (GI group), and stattic+global ischemia group (stattic+GI group). The global ischemia model was induced by modified four-vessel occlusion method. Stat3, p-stat3 and HIF-1α protein expressions in hippocampus were detected by Western blotting. MiR210 and HIF-1α mRNA expressions in cerebral cortex were detected by Real-time PCR. Results Compared with sham-operated group, stat3, p-stat3 and HIF-1α protein expressions in hippocampus and MiR210 and HIF-1α mRNA expressions in cerebral cortex of GI group significantly increased (all P<0.05). Compared with GI group, p-stat3 and HIF-1α protein expressions in hippocampus of stattic+ GI group significantly decreased, stat3 protein expression showed no statistical difference (P>0.05), and HIF-1α mRNA expression in cerebral cortex signifcantly decreased (P<0.05). Conclusion Expressions of MiR210 and stat3 have a marketable change after global ischemia reperfusion, and regulatory relationships may exist among stat3, MiR210 and HIF-1α.

Key words: Rat, MiR210, Signal transducer and activator of transcripation 3, Global ischemia, Hypoxia inducible factor-1α

CLC Number: 

  • R743.31
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