Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (9): 8-13.doi: 10.6040/j.issn.1671-7554.0.2020.0266

Previous Articles     Next Articles

Effects of PPARγ agonist rosiglitazone on the differentiation of beige adipocytes in 24 obese individuals

LI Han1,2, FU Tingting1,2, ZHANG Lei3, YAN Bing3, SUN Tao4, GUO Feng5, YIN Xiao1   

  1. 1.Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    4. Department of Thyroid Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    5. Department of Urology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Online:2020-09-10 Published:2020-08-30

PDF (PC)

337

Abstract: Objective To investigate the effects of peroxisome proliferators-activated receptor γ(PPARγ)agonist, rosiglitazone, on the browning process of white adipose tissues from obese patients, so as to provide possible treatment for obesity. Methods The subcutaneous white adipose tissues were collected from 24 obese patients who underwent selective operation. The adipose-derived stem cells(ADSCs)were isolated and induced to differentiate into mature adipocytes, which were then divided into control group, Rosi-1 group(1 μmol/L rosiglitazone), and Rosi-2 group(2 μmol/L rosiglitazone). The expressions of uncoupling protein 1(UCP1)and beige adipocytespecific thermogenic genes were detected with Western blotting and real time qRT-PCR. Lipolysis was analyzed using colorimetric assay. Results With rosiglitazone treatment, the adipocytes in Rosi-1 and Rosi-2 groups exhibited multi-nodular lipid droplets, higher expression of UCP1(t=23.12, P<0.01; t=7.35, P<0.01), and higher expressions of beige adipocyte specific thermogenic genes, including UCP1(t=2.63, P=0.03; t=9.86, P<0.01), PPARγ(t=2.8, P=0.02; t=11.06, P<0.01)and PRDM16(t=2.65, P=0.02; t=12.85, P<0.01). Rosi-1 group(t=2.76, P=0.02)and Rosi-2 group(t=5.83, P<0.01)showed increased lipolysis compared with control group. Conclusion PPARγ agonists can enhance the differentiation of beige adipocytes in white adipose tissues in obese patients and induce the browning process of white adipose tissues. The development of PPARγ agonists which have effects on adipose tissues may provide new path for the treatment of obesity.

Key words: Obesity, Peroxisome proliferators-activated receptor γ agonist, White adipose tissue, Browning, Subcutaneous adipose tissue

CLC Number: 

  • R589.2
[1] Mendenhall E, Singer M. The global syndemic of obesity, undernutrition, and climate change [J]. Lancet, 2019, 393(10173): 1-7.
[2] Castañeda D, Gabani M, Choi SK, et al. Targeting autophagy in obesity-associated heart disease [J]. Obesity(Silver Spring, Md.), 2019, 27(7): 1050-1058.
[3] Zeng J, Sauter ER, Li B. FABP4: A new player in obesity-associated breast cancer [J]. Trends Mol Med, 2020, 26(5): 1-4.
[4] 曹文悦,蒲迎,刘钊,等.成人脂肪细胞β3肾上腺素受体表达与肥胖的相关性[J]. 山东大学学报(医学版), 2018, 56(1): 81-85. CAO Wenyue, PU Ying, LIU Zhao, et al. Relationship between adipocyte β3 adrenergic receptor expression level and adiposity in adults [J]. Journal of Shandong University(Health Sciences), 2018, 56(1): 81-85.
[5] 金美玲, 孟凡彪, 赖奕宏, 等. 白藜芦醇抑制小鼠肥胖的功效及相关机制探讨[J]. 现代生物医学进展, 2018, 18(15): 2819-2823. JIN Meiling, MENG Fanbiao, LAI Yihong, et al. Resveratrol exerts anti-obesity effect involving modulation of UCP-1 and HO-1 [J]. Progress in Modern Biomedicine, 2018, 18(15): 2819-2823.
[6] Kim SY, Lee MS, Chang E, et al. Tartary buckwheat extract attenuated the obesity-induced inflammation and increased muscle PGC-1a/SIRT1 expression in high fat diet-induced obese rats [J]. Nutrients, 2019, 11(3): 654-667.
[7] Xie S, Li Y, Teng W. Liensinine inhibits beige adipocytes recovering to white adipocytes through blocking mitophagy flux in vitro and in vivo [J]. Nutrients, 2019, 11(7): 1640-1653.
[8] Paulo E, Wang B. Towards a better understanding of beige adipocyte plasticity [J]. Cells, 2019, 8(12): 1552-1567.
[9] Ruiz JR, Sánchez-Delgado G, Martínez-Téllez B, et al. Association between habitual physical activity and brown adipose tissue activity in individuals undergoing PET-CT scan [J]. Clin Endocrinol, 2015, 83(4): 590-594.
[10] 黄斌, 孟然, 冯斌, 等. 艾塞那肽对肥胖大鼠白色脂肪自噬及棕色化的影响[J]. 中国糖尿病杂志, 2016, 8(2): 81-86.
[11] Merlin J, Sato M, Chia LY, et al. Rosiglitazone and a β-adrenoceptor agonist are both required for functional browning of white adipocytes in culture [J]. Front Endocrinol(Lausanne), 2018, 9: 249. doi:10.3389/fendo. 2018. 00249.
[12] Narasimhan S, Weinstock RS. Youth-onset type 2 diabetes mellitus: lessons learned from the today study [J]. Mayo Clin Proc, 2014, 89(6): 806-816.
[13] Yang RZ, Lee MJ, Hu H, et al. Acute-phase serum amyloid A: an inflammatory adipokine and potential link between obesity and its metabolic complications [J]. PLoS Med, 2006, 3(6): 884-894.
[14] Choi SS, Park J, Choi JH. Revisiting PPARγ as a target for the treatment of metabolic disorders [J]. BMB Rep, 2014, 47(11): 599-608.
[15] Nierenberg AA, Ghaznavi SA, Sande Mathias I, et al. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha as a novel target for bipolar disorder and other neuropsychiatric disorders [J]. Biol Psychiatry, 2018, 83(9): 761-769.
[16] Soccio RE, Li Z, Chen ER, et al. Targeting PPARγ in the epigenome rescues genetic metabolic defects in mice [J]. J Clin Invest, 2017, 127(4): 1451-1462.
[17] Alvarez-Dominguez JR, Bai Z, Xu D, et al. De novo reconstruction of adipose tissue transcriptomes reveals long non-coding RNA regulators of brown adipocyte development [J]. Cell Metab, 2015, 21(5): 764-776.
[18] Shuai L, Zhang LN, Li BH, et al. SIRT5 Regulates brown adipocyte differentiation and browning of subcutaneous white adipose tissue [J]. Diabetes, 2019, 68(7): 1449-1461.
[19] Park SS, Lee YJ, Kang H, et al. Lactobacillus amylovorus KU4 ameliorates diet-induced obesity in mice by promoting adipose browning through PPARγ signaling [J]. Sci Rep, 2019, 9(1): 20152. doi:10.1038/s41598-019-56817-w.
[20] Qiang L, Wang L, Kon N, et al. Brown remodeling of white adipose tissue by Sirt1-dependent deacetylation of PPARγ [J]. Cell, 2012, 150(3): 620-632.
[21] Min BK, Kang HJ, Choi BJ, et al. Phenylbutyrate ameliorates high-fat diet-induced obesity via brown adipose tissue activation [J]. Biol Pharm Bull, 2019, 42(9): 1554-1561.
[22] Bartelt A, Heeren J. Adipose tissue browning and metabolic health [J]. Nat Rev Endocrinol, 2014, 10(1): 24-36.
[23] Vuppalanchi R, Chalasani N. Nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: Selected practical issues in their evaluation and management [J]. Hepatology, 2009, 49(1): 306-317.
[24] Fayyad AM, Khan AA, Abdallah SH, et al. Rosiglitazone enhances browning adipocytes in association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes [J]. Int J Mol Sci, 2019, 20(7): 1618. doi:10.3390/ijms20071618.
[25] Bae IS, Kim SH. Expression and secretion of an atrial natriuretic peptide in beige-like 3T3-L1 adipocytes [J]. Int J Mol Sci, 2019, 20(24): 6128. doi:10.3390/ijms20246128.
[26] Ross SA, Dzida G, Vora J, et al. Impact of weight gain on outcomes in type 2 diabetes [J]. Curr Med Res Opin, 2011, 27(7): 1431-1438.
[27] Eliasson B, Smith U, Mullen S, et al. Amelioration of insulin resistance by rosiglitazone is associated with increased adipose cell size in obese type 2 diabetic patients [J]. Adipocyte, 2014, 3(4): 314-321.
[1] HU Yanwen, WANG Zhiyuan, YU Wanjiang, ZHAO Huichen, HAN Heli, XU Zhipeng, MA Hong, ZHANG Yuchao, LIU Yuantao. Correlation of fat distribution with metabolic syndrome and glucose metabolism in 52 obese patients [J]. Journal of Shandong University (Health Sciences), 2020, 58(8): 101-106.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd