Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (9): 34-39.doi: 10.6040/j.issn.1671-7554.0.2020.0748

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Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells

SUN Yifeng1, GAO Yu2, LIANG Yongyuan1, GAO Yang1   

  1. 1. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Maternal and Child Health Care of Family Planning Service Center of Shibei District, Qingdao 266032, Shandong, China
  • Online:2020-09-10 Published:2020-08-30

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Abstract: Objective To investigate the expression of Complexin 2(CPLX2)in human hepatocellular carcinoma(HCC)and its effects on the proliferation and invasion of Huh7 cells. Methods The mRNA and protein expressions of CPLX2 in 30 HCC tissues and adjacent tissues were detected with RT-qPCR and Western blotting. Two CPLX2 small interfering RNA(siRNA)were transfected into Huh7 cells mediated by Lipofectamine 2000. The transfection efficiency of siRNA was confirmed by RT-qPCR and Western blotting. The cells were divided into 4 groups: blank group, siRNA control group, CPLX2 siRNA group 1 and CPLX2 siRNA group 2. The cell proliferation and migration were determined with methyl thiazol tetrazolium(MTT)and Transwell assay. The siRNA interference effects and cell invasion were analyzed with group t test. The cell proliferation in each group was compared with two-way ANOVA. Results The expression of CLPX2 was higher in HCC tissues than in adjacent tissues ［(22.69±14.78) vs(4.03±2.65), t=5.941, P<0.001］. The mRNA expression of CPLX2 was(0.34±0.02)in CPLX2 siRNA group 1 and(0.48±0.01)in CPLX2 siRNA group 2, which was lower than that in the siRNA control group(0.88±0.02)and blank group(1.00±0.05), and the difference was statistically significant(P<0.05), showing good siRNA interference effects. MTT assay confirmed the cell proliferation was lower in CPLX2 siRNA groups 1 and 2 than in the control group(P<0.001)and blank group(P<0.001). Transwell migration showed the number of penetrating cells in each detection field was(37.0±2.0)in CPLX2 siRNA group 1 and(46.3±2.5)in CPLX2 siRNA group 2, which was lower than that in the blank group(88.0±2.0)and siRNA control group(77.0±4.4). Transwell invasion showed the number of penetrating cells was(29.7±2.5)in CPLX2 siRNA group 1 and(41.0±2.6)in CPLX2 siRNA group 2, which was lower than that in the blank group(74.7±3.1)and siRNA control group(68.7±1.5), and the difference was statistically significant(P<0.001). Conclusion CPLX2 is highly expressed in HCC tissues and knockdown of CPLX2 can inhibit the proliferation and migration of Huh7 cells, indicating that CPLX2 may play an important role in HCC tumorigenesis and progression.

Key words: Hepatocellular carcinoma, Complexin 2, Proliferation, Migration

CLC Number: 

  • R735.7
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