Journal of Shandong University (Health Sciences) ›› 2020, Vol. 1 ›› Issue (7): 32-37.doi: 10.6040/j.issn.1671-7554.0.2020.0096

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Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion

MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui   

  1. Medical College of Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China
  • Online:2020-07-20 Published:2020-07-10

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Abstract: Objective To assess the relationship between miR-27b-3p and SMAD1 and to explore the effects of MiR-27b-3p on osteosarcoma cell proliferation, invasion and migration, so as to provide theoretical basis for targeted therapy of osteosarcoma. Methods Candidate target genes were predicted with bioinformatics methods. The targeting relationship between miR-27b-3p and SMAD1 was determined with dual luciferase report experiments. The SAOS-2 osteosarcoma cell line was selected with qRT-PCR. After SAOS-2 cells were transfected with miR-27b-3p, they were divided into 3 groups: miR-27b-3p inhibitor group, blank control group and negative control group. The effects of miR-27b-3p on SAOS-2 cells were detected with MTT, Transwell migration and invasion assay. The expression of SMAD1 after miR-27b-3p silencing was determined with Western blotting. Results Bioinformatics analysis showed there were binding sites of miR-27b-3p and SMAD1-UTR. Dual luciferase experiments showed the miR-27b-3p inhibitor group had higher expression of SMAD1 than the negative control group(P<0.05), and SMAD1 was the target gene of miR-27b-3p. MTT, Transwell migration and invasion assay showed the miR-27b-3p inhibitor group had reduced cell proliferation, migration and migration than the blank control group and negative control group(P<0.05). The miR-27b-3p inhibitor group had significantly lower protein expression of SMAD1 than negative control group(P<0.05). Conclusion miR-27b-3p can promote the proliferation, migration and invasion of osteosarcoma cells by regulating the expression of SMAD1, and may play a role as an oncogene in osteosarcoma cells.

Key words: Osteosarcoma, miR-27b-3p, SMAD1, Cell proliferation, Cell migration, Cell invasion

CLC Number: 

  • R738
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