JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 30-34.doi: 10.6040/j.issn.1671-7554.0.2014.688

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Effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780

ZHOU Jing1,2, ZHENG Yabing2, YAN Li2, CHANG Xiaotian2, ZHAO Shan2, RONG Fengnian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Gynecology and Obstetrics, Qianfoshan Hospital of Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-10 Revised:2014-11-11 Published:2014-12-10

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Abstract: Objective To investigate the effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780. Methods A2780 cells were cultured in medium with estrogen (10-12, 10-10, 10-8, 10-6, 10-4mmol/L). The expression of PADI4 was detected using real-time PCR and Western blotting after 96 hours. The cell proliferation was measured with CCK-8 assay. The concentration of estradiol-17β was chosen at which PADI4 expression changed the most significantly. A2780 cells were grouped into blank control group,single-agent estradiol-17β group, single-agent cis-platinum group and estradiol-17β plus cis-platinum group (combination group). The PADI4 expression and cell proliferation were measured by real-time PCR, Western blotting and CCK-8 assay. Results ① The PADI4 expression and cell proliferation decreased in A2780 cells treated with estradiol-17β in the range of 10-12mmol to 10-6mmol, but increased with higher concentration of estradiol-17β. A significant association was detected between PADI4 expression and cell proliferation. ② Compared with the blank control group, PADI4 expression decreased in cells cultured with estradiol-17β plus cis-platinum and single-agent cis-platinum group, and the proliferation of combination group was lower than that of the single-agent cis-platinum group. Conclusion Estradiol-17β regulates the expression of PADI4 and cell proliferation of ovarian cancer. The role of estradiol-17β may be associated with PADI4 expression.

Key words: Ovarian tumor, Peptidylarginine deiminase 4, Estradiol-17β, Cis-platinum, Cell proliferation

CLC Number: 

  • R711.75
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