Table of Content

  

10 December 2014
Volume 52 Issue 12

Detection of cross-linking degree in artificial aeart valves with amine-specific fluorescent probe

LI Zi, ZHANG Xuping, JIAO Haimiao, LIU Luqi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  1-4.  doi:10.6040/j.issn.1671-7554.0.2014.183

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Objective To detect the cross-linking degree of bioprosthetic valve with carboxyl fluorescein succinimide ester(CFSE), which can combine with amino groups specifically and irreversibly and emits green fluorescent in 488 nm excitation optical wavelength. Methods Decellularized bovine pericardium was soaked in 0.5% glutaraldehyde solution and divided into four groups:blank control group, 10 minutes group, 30 minutes group and 6 hours group. Each specimen was made from frozen section respectively and immersed in the solution of CFSE, as a fluorescent amine-reactive probe. The fluorescence image of every frozen section can be observed by fluorescence microscopy and average fluorescence intensity of each image was measured with image analysis software. At the same time, the protein which was extracted from each group's bovine pericardium was tested by SDS-PAGE to compare with the amino immune fluorescent probe technology. Results The number of cross-linking amino reduced gradually with increased cross-linking time, as reflected in the light intensity of the groups, control group > 10 minutes group>30 minutes group>6 hours group,with remarkable statistics significance among groups (P<0.01). Conclusion Compared with the SDS-PAGE method, amino immune fluorescent probe technology is more intuitive, sensitive, and it can reflect the crosslinking effect of valves organization at any part and any layer. The amino immunefluorescent probe technology may provide a new theoretical basis and train of thought for degree detection of artificial biological valve crosslinking.

Function of endogenous NT-3 in the regeneration sprouting of large neuron at late-stage spinal cord injury

WANG Xuyang, JIANG Dunqing, CHEN Shiwen, TIAN Hengli, LIU Fen, LI Zhiqiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  5-9.  doi:10.6040/j.issn.1671-7554.0.2014.190

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Objective To explore the function of endogenous neurotrophin-3 (NT-3) in the regeneration sprouting of large neuron at late-stage spinal cord following dorsal root rhizotomy in adult cats. Methods The 1st to the 5th lumbar (L₁-L₅), and the 7th lumbar (L₇) to the 2nd sacral (S₂) dorsal root ganlia (DRG) were exposed and removed from their respective intervertebral foramina, leaving the L₆ DRG untouched. Antibody of NT-3 was blocked. Two months later, CB-HRP transganglionic tracing, histochemistry and enzymohistochemistry analysis were performed to compare the spinal cord segments and the spared DRG. Results NT-3 positive immunity reaction (IR) mainly distributed in large-sized neurons of DRG and part of neurons in spinal lamina II. Two months after the operation, the number of NT-3-IR neurons in DRG significantly increased (P<0.05), but in lamina Ⅱ there was no difference compared to the control group. After CB-HRP tetramethyl benzidine (TMB) enzymohistochemistry coloration, the densities of axon terminalsprouting derived from spared DRG were more markedly increased in the operation group than in the control group. However, after antibody blockade of NT-3, the densities decreased sharply compared to that of operation group. Conclusion The regeneration sprouting of large sensory neuron of DRG at late-stage distribute mainly in the deep layers (Ⅲ, Ⅳ, Ⅴ layer) of spinal dorsal horn. Endogenous NT-3 may be involved in the spinal cord plasticity by up-regulating the expression of NT-3, which promotes the long-term repair of injured spinal cord.

Pancreatic pathological and biochemical changes in a Bama miniature pig model of type 1 diabetes mellitus

YU Jian, YE Yao, HUANG Lili, LIU Xiaoling, MO Rufen, YANG Fan, HU Jing, ZHOU Yingqiong, HE Yongling

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  10-14.  doi:10.6040/j.issn.1671-7554.0.2014.440

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Objective To explore the efficacy of multiply intraperitoneal injection of streptozotocin (STZ) on establishment of type 1 diabetes mellitus (T1DM) model of Bama miniature pigs. Methods Twelve healthy male Bama miniature pigs were divided into control group (n=6) and diabetic group (n=6). Diabetic group was injected intraperitoneally with 75mg/kg STZ and one week later, with 150 mg/kg STZ. The control group was injected with the equal volume of sodium citrate solution. Fasting blood glucose was monitored by Roche glucose meter. Oral glucose tolerance test(OGTT)and insulin release test (IRT) were performed at the end of the experiment. Pancreatic tissues were collected for histopathological examination and β cell immunohistochemical staining. Results ① All miniature pigs in the diabetic group were the successful T1DM models and showed the characters of polyuria, polydipsia, polyphagia and weight loss. ② Since the 13^th day after injection, fasting blood glucose of the diabetic group increased significantly and maintained at the level of (7.6-17.1) mmol/L. ③ One week after successful modeling, OGTT and IRT results indicated that the blood glucose level was higher and insulin was lower than the control group (all P<0.01). ④ The histopathological examination of pancreatic tissue pathology suggested that the numbers of pancreatic islets and β cells significantly decreased in the diabetic group. ⑤ Islet β cell immunohistochemical results suggested that the area of positive staining in the diabetic group was lower than that in the control group[(10.68±2.78)% vs (43.63±2.83)%, P<0.01]. Conclusion A new T1DM animal model is established by multiply intraperitoneal injection of STZ in Bama miniature pigs. The method has the advantages of simple operation, high success rate and safety.

Effects of PEDF on morphology and activity of rat retinal Müller cells cultured with high glucose

LI Yan, LI Mengyun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  15-19.  doi:10.6040/j.issn.1671-7554.0.2014.450

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Objective To investigate the effects of pigment epithelium-derived factor (PEDF) on the morphology and activity of high glucose-stimulated rat retinal müller cells in vitro, and explore the protective mechanism of PEDF on müller cells. Methods The rat retinal müller cells were divided into control group, high glucose (HG) group and high glucose coordinated PEDF (HG+PEDF) group. Retinal müller cells were cultured and purified by repeated pancreatic enzyme-degisting method. The purity of retinal müller cells was identified by double staining immunofluorescence techniques. The morphology was identified by inverted phase contrast microscope and transmission electron microscopy. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were studied with CCK-8 kit, SOD kit and MDA kit in a quantitative way. Results OD values of control group, HG group and PEDF+HG group were 0.67±0.03, 0.54±0.03 and 0.63±0.03, respectively. SOD activity of these groups were (196.69±22.15), (153.42±15.54), and (180.32±16.47) U/mg prot. MDA level of these groups were (2.11±0.43), (2.86±0.33), and (2.40±0.28) nmol/mg prot. When retinal müller cells were cultured with high glucose, cells morphology changed significantly, cells activity and SOD activity decreased (P<0.01), while MDA level obviously increased (P<0.01).When PEDF were added, the change of cells morphology reduced, cells activity and SOD activity increased obviously(P<0.05), while MDA level decreased (P<0.05). Conclusion High glucose can damage the morphology and activity of retinal müller cells. PEDF can protect retinal müller cells from the damage of glucose.

Hypoglycemic mechanism of volatile oil of plateau plant Brassica rapa L. in hyperlipidemic and hyperglycemic mice

CHEN Xianghong, LIU Yan, WENG Yuxin, KANG Wenjuan, YANG Shibing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  20-23.  doi:10.6040/j.issn.1671-7554.0.2014.172

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Objective To explore the hypoglycemic mechanism of plateau plant Brassica rapa L. (turnip) volatile oil in an animal model of diabetic mice. Methods The volatile oil was extracted using supercritical carbon dioxide extraction method. The diabetic mice model was built by feeding high-fat and high-carbohydrate diet for 4 weeks then intraperitoneal injection of streptozotocin (STZ) 100 mg/kg for 3 days. The mice with fasting blood glucose in tail vein being greater than 11.0 mmol/L were defined as successful experimental models. Fifty mice were randomly divided into control group, model group, high dose of turnip volatile oil group, low dose of turnip volatile oil group, and positive control (metformin hydrochloride) group, 10 in each group. The mice were perfused for 14 days at one time per day. The blood was collected from tail vein and the blood glucose level was measured before and after building the models, and at day 3, 7, 14 after medication. The blood samples were taken from the mice's eyeballs and the glycosylated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), and serum insulin (INS) levels were measured. The fresh spleen and thymus gland were cut and weighed to compute organ index. Results Both high dose and low dose of turnip volatile oil had significant influences on the type 2 diabetic model mice, including reduced blood glucose (P<0.01) and HbA1c levels (P<0.05), increased INS level (P<0.05), and decreased TG and TC levels (P<0.01). The turnip volatile oil could protect the thymus in diabetic mice, and enhance the immune ability. Conclusion Turnip volatile oil can reduce hyperglycemia significantly in diabetic model induced by STZ, which may due to the intervention of the biosynthesis of HbAlc and increase of INS level.

Effect of insulin sensitizer on the expression of insulin receptor substrate in endometrium of rats with polycystic ovarian syndrome

WANG Wei, CHANG Kang, LI Xiaodong, HAO Guimin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  24-29.  doi:10.6040/j.issn.1671-7554.0.2014.670

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Objective To explore the expressions of insulin receptor substrate (IRS) 1, 2 and phosphorylation of tyrosine phosphokinase in the endometrium of rats with polycystic ovarian syndrome (PCOS) after metformin treatment. Methods The PCOS models were induced by Poretsky method and randomized to the model control group (n=20) and metformin group (n=20). A total of 10 SD rats were enrolled in the blank control group. The expressions of IRS-1, 2 and phosphorylation of tyrosine phosphokinase were analyzed with immunohistochemistry. Results The expressions of IRS-1, IRS-2, P-tyr(IRS-1) and P-tyr(IRS-2) proteins in the endometrium of the metformin group were significantly higher than those of the model control group (P<0.01), but still lower than those in the blank control group (P<0.05). Conclusion There is a selective insulin resistance in the endometrium of PCOS rats, which may be related to abnormal tyrosine phosphorylation and protein expressions of insulin receptor substrates. Metformin improves tyrosine phosphorylation and protein expressions of IRS-1and IRS-2 in the endometrium and partly corrects insulin resistance.

Effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780

ZHOU Jing, ZHENG Yabing, YAN Li, CHANG Xiaotian, ZHAO Shan, RONG Fengnian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  30-34.  doi:10.6040/j.issn.1671-7554.0.2014.688

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Objective To investigate the effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780. Methods A2780 cells were cultured in medium with estrogen (10^-12, 10^-10, 10^-8, 10^-6, 10^-4mmol/L). The expression of PADI4 was detected using real-time PCR and Western blotting after 96 hours. The cell proliferation was measured with CCK-8 assay. The concentration of estradiol-17β was chosen at which PADI4 expression changed the most significantly. A2780 cells were grouped into blank control group,single-agent estradiol-17β group, single-agent cis-platinum group and estradiol-17β plus cis-platinum group (combination group). The PADI4 expression and cell proliferation were measured by real-time PCR, Western blotting and CCK-8 assay. Results ① The PADI4 expression and cell proliferation decreased in A2780 cells treated with estradiol-17β in the range of 10^-12mmol to 10^-6mmol, but increased with higher concentration of estradiol-17β. A significant association was detected between PADI4 expression and cell proliferation. ② Compared with the blank control group, PADI4 expression decreased in cells cultured with estradiol-17β plus cis-platinum and single-agent cis-platinum group, and the proliferation of combination group was lower than that of the single-agent cis-platinum group. Conclusion Estradiol-17β regulates the expression of PADI4 and cell proliferation of ovarian cancer. The role of estradiol-17β may be associated with PADI4 expression.

Effect and mechanism of isorhynchophylline on platelet-derived growth factor(PDGF)-BB-induced pulmonary artery smooth muscle cell proliferation

ZHANG Xin, GUO Haipeng, WANG Zhenhong, TANG Linna, YANG Jie, WU Dawei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  35-40.  doi:10.6040/j.issn.1671-7554.0.2014.575

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Objective To investigate the effect of isorhynchophylline on platelet-derived growth factor (PDGF)-BB-induced pulmonary artery smooth muscle cells (PASMCs) proliferation and the related mechanism. Methods In PDGF-BB-induced PASMCs proliferation model, the effect and cytotoxicity of isorhynchophylline on PDGF-BB-induced PASMCs proliferation were measured by MTT assay and trypan blue exclusion, and the cell cycle progression was measured by flowcytometry. The expression of cell cycle-related protein and PDGF receptor β (PDGF-Rβ) signaling pathway in PDGF-BB-stimulated PASMCs were measured by Western blotting. Results Compared with the controls, the number of PASMCs increased approximately 2.5 fold after cultured with 20 ng/mL PDGF-BB for 24 h. Isorhynchophylline inhibited PDGF-BB-induced PASMCs proliferation in a concentration-dependent manner. 25 μmol/L isorhynchophylline inhibited the increase of PDGF-BB-induced PASMCs S phase proportion. Western blotting results indicated that isorhynchophylline decreased PDGF-BB-induced expressions of cyclin D1 and CDK6, and inhibited p27^kip1 degradation. Furthermore, isorhynchophylline negatively regulated PDGF-BB-induced phosphorylation of PDGF-Rβ and its downstream signaling molecules, including AKT and glycogen synthase kinase (GSK) 3β. Conclusion Isorhynchophyl-line inhibited PDGF-BB-induced PASMCs proliferation via inducing G₀/G₁ phase arrest, the possible mechanism of which is that isorhynchophylline modulate synthesization and degradation of cell cycle-related protein by inhibiting PDGF-Rβ and its downstream signaling pathway.

Preliminary study in the protective role of p120 catenin in inflammatory lung injury

SUI Xiangyu, ZHANG Xiangchun, ZHANG Guangyong, HU Sanyuan, WANG Yanlei, DAI Yong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  41-44.  doi:10.6040/j.issn.1671-7554.0.2014.538

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Objective To investigate the role of p120 catenin in inflammatory cytokine tumor necrosis factor-α (TNF-α)-induced lung microvascular endothelial cells activation, and explore the underlying mechanism. Methods p120 expression level in endothelial cells was downregulated with specific p120 small interference RNA (siRNA). Difference of TNF-α-induced nuclear factor-κB (NF-κB) activation, intracellular adhesion molecular-1(ICAM-1) expression, and neutrophil transendothelial migration were detected. Mechanism of p120's regulatory role in inflammatory lung injury with specific NF-κB inhibitor was investigated. Results TNF-α reduced p120 level in lung microvascular endothelial cells. p120 downregulation in endothelial cells significantly aggravated TNF-α-induced NF-κB activation, ICAM-1 expression, and neutrophil transendothelial migration. These effects were remarkably suppressed by NF-κB inhibitor. Conclusion p120 plays a critical protective role in TNF-α-induced lung microvascular endothelial cells activation and subsequent neutrophil transendothelial migration through the inhibition of NF-κB activation.

A new method of inducing adipose-derived stem cells into cartilage tissue

SHI Yehua, CHEN Zhipeng, MA Qikui, JU Ruihua, WANG Qirong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  45-49.  doi:10.6040/j.issn.1671-7554.0.2014.113

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Objective To rexplore a new method of inducing adipose-derived stem cells (ADSCs) into cartilage tissue. Methods ADSCs were isolated from rat adipose tissues and characterized by cell-surface markers CD90 and CD44. The passage-6 cells were seeded in alginate beads and induced into chondrocytes in medium containing TGF-β1. After 2 weeks of culture, the cells in alginate beads were released and induced for another 2 weeks. After the induced tissues were digested by type Ⅱ collagenase, the cells were harvested and identified, and expression of type Ⅱ collagen protein was detected by cell immunohistochemistry. Results Adipose-derived stem cells exhibited a fibroblast-like morphology and were positive for typical MSC-related markers CD44 and CD90. The expression of typeⅡcollagen protein in cells of induced tissues showed that it was a new method of chondrogenic engineering to induce adipose-derived stem cells released from alginate. Conclusion Chondrogenic differentiation of cells released from ADSCs-alginate beads after 2 weeks of chondrogenic inducing is a new method of cartilage tissue engineering.

Effects of ALA-PDT on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells

LIU Yuanyuan, WANG Yifei, ZHANG Chunmin, LIU Yao, WEI Guo, ZHANG Chunhong, LIU Ying

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  50-53.  doi:10.6040/j.issn.1671-7554.0.2014.021

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Objective To investigate the effect of aminolevulinic acid hydrochloride (ALA)-photodynamic therapy (PDT) on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells. Methods A431 cells were cultured in vitro. The proliferation and apoptosis of A431 cells with different drug concentration, different incubation time, different light dose, as well as with drug and light alone, were assessed by MTT. Hacat was as control. The mRNA expressions of Caspase-3 and Caspase-7 were detected by Real-time PCR. The protein expressions of Caspase-3 and Caspase-7 in A431 cells and Hacat cells were detected by Western blotting. Results The mRNA and protein expressions of Caspase-3 and Caspase-7 were lower in A431 cells than in the Hacat cells with statistically significant difference (P<0.05). ALA-PDT inhibited the proliferation of A431 cells in a certain extent, and increased the mRNA and protein expressions of Caspase-3 and Caspase-7 (P<0.01). The inhibition on A431 cells could reach the maximum when the light dose was 80 J/cm² with the same incubation time and concentration of ALA. Conclusion ALA-PDT can induce the expressions of Caspase-3 and Caspase-7 directly or indirectly, which induce the apoptosis of cutaneous squamous cell carcinoma A431 cells.

Centrosome isolation and analysis by mass spectrometry-based proteomics

ZHONG Kaize, ZHAO Jian, YUAN Shoudao, WANG Luyu, JING Yanjie, HAO Tong, CHEN Jianxing, ZHAN Qimin, JIANG Wei, ZHU Changjun, PAN Lina

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  54-59.  doi:10.6040/j.issn.1671-7554.0.2014.146

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Objective To identify the composition of centrosome proteins by human centrosome extraction, purification and protein spectrum analysis. Methods Centrosomes in K562 cells were extracted through density gradient centrifugation. Protein spectrum analysis was carried out on the sample tested by Western blotting. System analysis of all these centrosome proteins were proceeded by Gene Ontology (GO). Results The human centrosome was successfully extracted and purified. Cell lysis fractions where centrosome-associated protein γ-Tubulin accumulated were detected. There were no mitochondrial protein VDAC1 and ribosomal protein RPL15 in these fractions. The centrosome proteincomponents, including cytoplasm material transport protein, cytoskeleton protein, membrane combining protein, kinase protein and many other protein molecules, covered a variety of the biological message pathways within cells. Conclusion Centrosomes in different cell growth periods can be extracted and purified through this method. Combining protein spectrum analysis, this study is helpful to understand the basic structure and functions of the centosome.

Clinical efficacy of orthotopic heart transplantation for 40 patients

WANG Yanchi, LIU Tianqi, WANG Dong, LI Quan, LI Peijie, BI Yanbin, LI Min, XU Li, MA Yanping, WANG Minghua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  60-63.  doi:10.6040/j.issn.1671-7554.0.2014.353

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Objective To summarize and analyze the clinical efficacy and experiences of orthotopic cardiac transplantation for end-stage heart disease. Methods Data of 40 patients underdoing orthotopic heart transplantation in our center in the past 10 years were retrospectively reviewed. The enrollments included 29 cases of dilated cardiomyopathy, 6 cases of ischemic heart disease, 4 cases of hypertrophic cardiomyopathy, and 1 case of restrictive cardiomyopathy. Immunosuppressive therapy with cyclosporine A, corticosteroid, and mycophenolate mofetile was adopted. Thirty-five patients received induction therapy with Tac monoclonal antibody or basiliximab monoclonal antibody. According to trough concentration (C0) and peak concentration (C2), the dose of cyclosporine A was regulated. Results The rate of successful operation was 95% (38/40). The 1-, 3-, 5- year survival rate was 90%, 82.5%, and 77.5%. Main causes of death were right heart failure, fungal infection, and graft failure. Acute rejection occurred in 7 cases (17.5%), and all recovered after cortieasteriod impulse therapy. Three cases were diagnosed as rejection after C0 and C2 were monitored spontaneously. Conclusion It's reliable to treat patients with end-stage heart disease with orthotopic cardiac transplantation. Monitoring C0 and C2 can control the cyclosporine concentration within the valid range and avoid occurrence of early acute rejection.

Correlation analysis of type 2 diabetes with diabetes family history and hypertension

LIU Li, SHAO Yuhan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  64-68.  doi:10.6040/j.issn.1671-7554.0.2014.406

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Objective To explore whether diabetes family history and hypertension have an interaction effect on the incidence of type 2 diabetes. Methods A total of 791 newly diagnosed patients with type 2 diabetes (T2DM) screened by our investigation were chosen as the case group, and 4 374 people with normal blood glucose in the same investigation were enrolled as the control group. The prevalence of diabetes family history and hypertension of the two groups were compared, and their correlation with T2DM was analyzed with an additive model. Results The prevalence of diabetes family history (χ² = 40.1, P<0.001) and hypertension (χ²=145.4, P<0.001) of the case group were higher than those of the control group. After adjusting confounding factors by using a multiple Logistic regression model, the synergy index was 1.19, the relative excess risk due to interaction was 0.52, the attributable interaction percentage was 12.20%, and the pure attributable interaction percentage was 15.71%. Conclusion Diabetes family history and hypertension have a positive effect on the incidence of type 2 diabetes, and simultaneous existence of the two can lead to high risk of type 2 diabetes.

Clinical characteristics and pregnancy outcomes of patients with systemic lupus erythematosus: a study of 66 cases with 67 pregnancies

WANG Likui, SANG Hongai, MA Yuyan, XU Yintao, WANG Linlin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  69-72.  doi:10.6040/j.issn.1671-7554.0.2014.772

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Objective To investigate the clinical characteristics and pregnancy outcomes of patients with systemic lupus erythematosus (SLE). Methods Clinical datas of 66 pregnant women with 67 pregnancies (36 cases in flare group and 31 cases in non-flare group) with SLE treated in our hospital during Aug. 2005 and Aug. 2014 were collected and retrospectively analyzed. Results The incidence rates of preterm birth, small for gestational age infant, therapeutic abortion, pregnancy loss and severe preeclampsia increased significantly in the flare group compared with the non-flare group (all P<0.05). SLE was mostly active in the middle and late pregnancy, with the highest rate of proteinuria and skin rash, which were involved with multi-organ and multi-system damage, especially kidney damage. SLE flare in early pregnancy harmed the blood system, with or without skin rash. Conclusion SLE flare can lead to damage of the blood system, kidney and increased risks of poor fetal and maternal outcomes. Thus, for a better outcome of lupus pregnancy, it is essential to make a thoughtful plan, ensure a stable condition for at least 6 months before conception, and monitor the clinical indexes closely during pregnancy.

Evaluation of fetal myocardial mechanics of left ventricular in pregnancy-induced hypertension syndrome using velocity vector imaging

LIU Fang, TAO Guowei, JIANG Lili, LIU Shaoping, SHI Linlin, DING Tingting, GAO Shengjie, MA Zhe

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  73-77.  doi:10.6040/j.issn.1671-7554.0.2014.743

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Objective To explore the most valuable parameters for fetal myocardial mechanics by evaluating fetal left ventricular myocardial mechanics in women with pregnancy-induced hypertension syndrome using velocity vector imaging (VVI). Methods The study involved two groups of pregnant women: the hypertensive group (women with pregnancy-induced hypertension, n=36) and control group (healthy subjects, n=54). The ventricular systolic and diastolic segmental and global peak velocity, strain, and strain rate were evaluated with VVI and compared between the two groups. Results Systolic and diastolic segmental peak velocity, strain and strain rate of the hypertensive group were lower than those of the control group. There were statistical differences in some of the segments (P<0.05). ② Systolic and diastolic global peak velocity, strain and strain rate of the hypertensive group were lower than those of the control group (P<0.05). Conclusion VVI can sensitively reflect the fetal myocardial mechanics in women withpregnancy-induced hypertension syndrome. Global strain and strain rate may be more sensitive than segmental strain and strain rate in the assessment of fetal myocardial mechanics.

Endoscopic band ligation of upper gastrointestinal submucosal lesions: long-term outcomes and risk factors

LIU Fengyan, HAN Bing, ZHONG Ning, ZHENG Xueting, LI Wenjie, HAN Wei, JIA Xiaoqing, LI Yanqing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  78-82.  doi:10.6040/j.issn.1671-7554.0.2014.282

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Objective To evaluate the long-term outcomes after endoscopic band ligation of upper gastrointestinal submucosal lesions and risk factors. Methods Clinical and endoscopic data of 194 cases of upper gastrointestinal submucosal lesions undertaking endoscopic band ligation were retrospectively analyzed. The success rate of treatment and adverse effect were obtained during long-term follow-up. The accrate rate of common endoscopy in evaluating the depth of lesions was assessed according to endoscopic ultrasound reports. Results The ligation was accomplished in 184 cases (94.8%), 90% of which were successful. Multivariable analysis showed that lesion diameter of more than 0.8cm and deep location were risk factors for the failure of ligation procedure. Evaluation of the depth of lesions by common white light endoscopy and endoscopic ultrasound was not comparable (Kappa value 0.04). Conclusion Endoscopic ligation of submucosal lesions in upper digestive tract is safe and effective, yet follow-up is necessary even for those who were thought to achieve success by the operators. And endoscopic ultrasound evaluation is essential before the procedure.

Correlation between liver functional scores, FibroScan measurement and histological subclassification of cirrhosis

LI Jiye, WANG Wei, PAN Runhua, A Sileng, LIAO Caixian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  83-88.  doi:10.6040/j.issn.1671-7554.0.2014.435

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Objective To investigate the relationship between histological subclassification of cirrhosis and liver functional scores as well as FibroScan transient elastography. Methods Clinical data of 60 biopsy-proven cirrhosis cases with different severity were collected and respectively reviewed. The liver functional scores and FibroScan values were assessed. Histology of cirrhosis was blindly subclassified using the Laennec fibrosis scoring system semi-quantitatively. Results Sixty cases were classified into 3 groups: mild cirrhosis group(n=27, 45%), moderate cirrhosis group(n=21, 35%) and severe cirrhosis group(n=12, 20%). FibroScan values of mild, moderate and severe cirrhosis groups were (14.90±5.54), (25.23±10.11) and (33.99±13.55)kPa, respectively, with significant difference among the groups (P<0.05). The areas under the receiver operating characteristic (ROC) curve of FibroScan were 0.908 for moderate to severe cirrhosis, and 0.865 for severe cirrhosis. Child-Pugh scores of the three groups were 5.61±1.14, 6.05±1.21 and 5.74±0.93, respectively, with no significant difference among them (P>0.05). MELD scores of the three groups were 5.90±4.22, 7.14±5.33 and 7.03±5.13, respectively, with no significant difference (P>0.05). Conclusion FibroScan is a valuable examination for liver cirrhosis. There is no correlation between liver functional scores and histological subclassification of cirrhosis.

Application value of diffusion-weighted MR imaging in estimating the histological grade of hepatocellular carcinoma

GUO Weihua, ZHAO Suhong, PANG Guodong, SHAO Guangrui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  89-93.  doi:10.6040/j.issn.1671-7554.0.2014.594

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Objective To evaluate the significance of magnetic resonance diffusion-weighted imaging (DWI) in predicting the histological grading of hepatocellular carcinoma (HCC). Methods Data of 27 patients with 27 surgically removed HCC who underwent preoperative conventional MRI and DWI examinations were retrospectively reviewed. The relationship between visual signal intensity and apparent diffusion coefficient (ADC) with histological differentiation of HCC was analyzed. Results Histopathologically, the tumors were classified into well-differentiated (n=6), moderately-differentiated (n=10) and poorly-differentiated (n=11). On DWI, 1 of the 27 HCCs appeared isointense, 9 slightly hyperintense, and the remaining 17 tumors hyperintense to the surrounding liver. Statistical analysis revealed no significant difference between visual signal intensity and histopathologic grade (P=0.758). The mean ADC value of the poorly-differentiated HCCs[(1.16±0.16)×10^-3mm²/s] was significantly lower than that of the well-differentiated HCCs[(1.43±0.09)×10^-3mm²/s] (P=0.004) and moderately-differentiated HCCs [(1.34±0.19)×10^-3mm²/s] (P=0.023). There was a significant correlation between the differentiation and the ADC value of the HCCs (r=-0.566, P=0.002). Conclusion DWI with ADC measurement of HCC may be a valuable imaging tool to noninvasively predict the differentiation of HCC. However, the histopathological grades of HCC are not correlated with the visualsignal intensity.

Application value of spectral CT imaging in differential diagnosis of hepatocellular carcinoma and hepatic mass-type cholangiocarcinoma

LIU Changxu, ZHANG Chengqi, WANG Xinyi, YANG Zhiqiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  94-98.  doi:10.6040/j.issn.1671-7554.0.2014.494

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Objective To explore the application value of spectral CT imaging in the differential diagnosis of hepatocellular carcinoma and hepatic mass-type cholangiocarcinoma. Methods A total of 34 liver cancer cases, including 20 hepatocellular carcinoma and 14 hepatic mass-type cholangiocarcinoma, underwent spectral CT scan. Iodine concentration, water concentration and 101 sets of monochromatic images were generated with gemstone spectral imaging post-processing software. The iodine concentration, water concentration, spectrum energy curve and normalized iodine concentration were measured at the monochromatic energy 55 Kev. The results were analyzed with independent samples t test. Results In hepatocellular carcinoma, the iodine concentration, spectrum energy curve and normalized iodine concentration were (12.84±2.38), (1.58±0.24) and (0.15±0.05) μg/cm³; while in cholangiocarcinoma, those were (10.71±3.01), (1.34±0.32) and (0.12±0.34) μg/cm³. There were significant differences between those indexes (P<0.05). The water concentration in hepatocellular carcinoma and cholangiocarcinoma were (1 041.05±6.47) and (1038.74±8.54) mg/cm³, respectively, exhibiting no significant difference (P>0.05). Conclusion Spectral CT is helpful for the differential diagnosis of hepatocellular carcinoma and hepatic mass-type cholangiocarcinoma.

Nutritional risk screening of hospitalized patients and effect evaluation of nutrition therapy

MENG Yan, ZHANG Jie, YUAN Yuan, ZHANG Jitian, CHEN Liyong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  99-102.  doi:10.6040/j.issn.1671-7554.0.2014.409

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Objective To understand the nutritional status of hospitalized patients and to assess the treatment effect of hospitalized patients with enteral nutrition support. Methods The nutritional status of 151 hospitalized patients were evaluated by Nutrition Risk Screening 2002 (NRS2002). The indexes before and after treatment such as gastrointestinal symptoms, prealbumin, total protein, albumin, globulin and retinol binding protein for patients of nutritional risk were tested. Results The incidence of nutritional risk of elderly patients (96.5%) was significantly higher than that of young and middled-aged patients (80.3%)(P=0.001). The gastrointestinal symptoms of patients improved significantly after treatment(P<0.001). The levels of prealbumin, total protein, albumin, and retinol binding protein were improved after treatment (all P<0.01). The level of globulin was improved after treatment but the difference was statistically nonsignificant (P>0.05). The difference of nutritional risk rate of research objects before and after treatment (89.4% vs 80.8%) was statistically significant (P=0.036). Conclusion NRS2002 can reflect the nutritional risk status of hospitalized patients conveniently and quickly. Nutritional therapy can reduce the incidence of nutritional risk to some extent and improve the nutritional status of patients.

Correlation between dental anxiety and eysenck personality among college students

QIU Yubei, WU Yaohui, FAN Yanni, GUO Weizhong, PENG Jiamei, REN Huiping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  103-106.  doi:10.6040/j.issn.1671-7554.0.2014.369

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Objective To investigate the distribution of dental anxiety (DA) among college students in Fuzhou City and reveal the correlation between DA and eysenck personality. Methods A survey with the Modified Corah's Dental Anxiety Scale and Eysenck Personality Questionnaire was conducted among 1,200 college students. Data collected were analyzed with software SPSS 17.0. Results The prevalence rate of DA was 55.7%. DA was negatively correlated to extroversion (r=-0.458) and psyehoticism (r=-0.380), and positively correlated to neuroticism (r=0.476). Students with DA had significantly higher scores of neuroticism and lower scores of extroversion and psyehoticism than students without DA (P<0.05). Conclusion Dental anxiety is correlated to eysenck personality. People who have higher score of neuroticism, and lower scores of extroversion and psyehoticism are more likely to suffer from dental anxiety. The trends of eysenck personality in people with DA are: low psyehoticism, introversion and unstable neuroticism.

Spacial distribution of fertility rate in Shandong Province and its influencing factors

LI Qi, SUN Yixin, LI Yixue, ZHANG Cuiqin, WU Rongshan, XUE Fuzhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(12):  107-112.  doi:10.6040/j.issn.1671-7554.0.2014.323

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Objective To analyze the spatial distribution of fertility rate in Shandong Province and its influencing factors. Methods Spatial analysis software GIS and GeoDA were used to build the spatial database of fertility rate of 139 counties in Shandong Province, and spatial autocorrelation analysis and global spatial regression model analysis were conducted. Results The global spatial autocorrelation analysis showed Moran's I=0.46, P<0.01. In the spatial error model analysis, marriage rate, dependency ratio, sex ratio, high education rate and divorce rate showed statistical significance (P<0.05), in which only the regression coefficient of the divorce rate was less than zero. Conclusion There is a certain spatial correlation and clustering in fertility rate among counties in Shandong Province. Weihai, Yantai and surrounding areas have lower aggregation in fertility rate, while Linyi and surrounding areas have higher aggregation in fertility rate. Fertility rate is positively related to the marriae rate, dependency ratio and sex ratio, and negatively related to divorce rate.