Table of Content

  

10 November 2014
Volume 52 Issue 11

Overview of the history of endoscopic endonasal surgery and multilayer skull base reconstruction

ZHAN Rucai, WANG Donghai, WANG Xinyu, LI Xingang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  1-5.  doi:10.6040/j.issn.1671-7554.0.2014.328

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Luteolin activates Nrf2 signalling pathway and confers protection against H₂O₂-induced toxicity in EA.hy926 cells

CHEN Hongzhong, LI Haizhen, REN Dongmei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  6-10.  doi:10.6040/j.issn.1671-7554.0.2014.429

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Objective To investigate the effect of luteolin on nuclear factor erythroid-2 related factor 2 (Nrf2) signalling pathway, and evaluate the protection on H₂O₂-induced toxicity in human umbilical vein EA.hy926 cells. Methods Cells were divided into control, positive drug (sulforaphane) treated, H₂O₂ treated, luteolin with different concentrations (5,10,20,40 μmol/L) treated, and luteolin with different concentrations (5,10,20,40 μmol/L) and H₂O₂ cotreated groups. ARE luciferase reporter gene assay, Western blotting analysis and qRT-PCR assay were used for identification of luteolin on activiting Nrf2 signalling pathway; flow cytometry with a fluorescent probe uploading was adopted for detecting the intracellular reactive oxygen species (ROS) levels; MTT assay was carried out for evaluating the protection against H₂O₂-induced toxicity. Results Luteolin increased the luciferase activity dose-dependently, enhanced both the protein and mRNA levels of Nrf2 and its downstream gene heme oxygenase-1 (HO-1), quinone reductase enzyme NAD(P)H: quinone oxidoreductase 1 (NQO1) and glutamate cysteine ligase modifier subunit (GCLM), and decreased intracellular ROS levels. Co-treatment with luteolin improved cell survival compared with H₂O₂ treatment alone. Conclusion Luteolin activates Nrf2 signalling pathway in EA.hy926 cells, inhibits H₂O₂-induced cytotoxicity and confers protection against oxidative-induced injury in EA.hy926 cells.

Inhibitive effect of EZH2-specific shRNA on SW480 cells and the impact of 5-FU

LV Yanfeng, JIANG Hua, WANG Jianxin, ZHANG Chengbo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  11-15.  doi:10.6040/j.issn.1671-7554.0.2014.263

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Objective To investigate the inhibitive effect of EZH2-specific shRNA on human colon cancer cell line SW480 and the impact of 5-FU on this inhibitive effect. Methods SW480 cells were transfected with EZH2-specific shRNA in vitro, then EZH2 mRNA and protein expressions were detected with RT-PCR and Western blotting, and cell proliferation was examined with MTT. The EZH2-specific shRNA expressing SW480 cells were implanted to nude mice and treated with 5-FU. After that, the levels of EZH2 mRNA expressed by tumor tissues were measured with RT-PCR. Results Compared with that of the negative control group, the EZH2 mRNA expression (P<0.01) and protein expression (P<0.05) of the gene-silencing group markedly decreased. The cell growth of the gene-silencing group was inhibited compared with that of the control group (P<0.05). In nude mice, the tumor volume, tumor weight and EZH2 mRNA expression of the 5-FU gene-silencing group and of the gene-silencing group remarkably decreased compared with those of the normal group (P<0.01). The EZH2 mRNA expression of the 5-FU gene-silencing group significantly decreased compared with that of the gene-silencing group (P<0.05). Conclusion The EZH2-specific shRNAplays a significant inhibitive role on EZH2 mRNA and protein expressions in SW480 cells both in vitro and in vivo. 5-FU can significantly inhibit the EZH2 mRNA expression of EZH2-gene-silencing SW480 cells in vivo.

Safety of reconstituted bone xenografts on neuronal cells

WANG Xianmei, LI Na, SUN Xiaoxia, LIU Chenghu, HOU Li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  16-20.  doi:10.6040/j.issn.1671-7554.0.2014.039

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Objective To evaluate the clinical safety of reconstituted bone xenografts on the nervous system by studying the influence on neuronal cell viability and apoptosis in Wistar rats. Methods Hippocampal and cortical neurons cells were cultured in the reconstituted bone xenograft extracts (preparation of liquid extracts was in accordance with ISO 10993.12-2002). The neuronal cells were divided into two groups: the reconstituted bone xenograft group, and the negative control group. Tests were conducted to study the viability and apoptosis of neuronal cells in different contact time and culture time with MTT incorporation assay and flow cytometry. Results There were no significant differences in neuronal cell viability and apoptosis between the two groups (P >0.05). Conclusion The reconstituted bone xenografts have no obvious influence on neuronal cell viability and apoptosis in Wistar rats. The result of this study may provide an experimental and theoretical basis for the safe clinical application of reconstituted bone xenografts.

Effects of Aspergillus fumigatus extract on human bronchial epithelial cells repair ability

LONG Fei, SU Lili, LIANG Bin, JIN Xianqiao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  21-26.  doi:10.6040/j.issn.1671-7554.0.2014.506

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Objective To investigate the effects of aspergillus fumigatus extract (AFE) on the human bronchial epithelial cells repair ability and its possible mechanism. Methods The cells were divided into medium control group, different concentrations (10, 20 and 40 mg/L) of AFE groups and heat-treated AFE group. Human bronchial epithelial cells 16HBE were cultured in vitro, which were exposed to different concentrations of AFE for different time. Wound closure assay, cell migration, quantitative cell attachment assay and spreading of bronchial epithelial cells were observed. AFE and heat-treated AFE were used to investigate the effects of AFE on the human bronchial epithelial cells repair ability. Results Compared with medium control group, the repair capacity, migration ability and adhesion ability of 16HBE cells were inhibited significantly in 20 and 40 mg/L groups (P<0.05) with a time- and concentration-dependent manner; however, there was no obvious change in 10 mg/L AFE group (P>0.05). The spreading ability of 16HBE cells in 10, 20 and 40 mg/L AFE groups were inhibited with a concentration-dependent manner (P<0.05). Heat-treated AFE, whose protease activities were lost, showed no effects on the indicators mentioned above (P<0.05). Conclusion AFE, depending on its protease activity, can inhibit the repair ability of human bronchial epithelial cells, which is positively related to the exposure time and concentration of AFE.

Fertility restore of allotransplantation of vitrified-warmed junvenile ovaries treated with a modified vitrification solution

WANG Hongyan, WANG Yanrong, XU Xian, ZHAO Junli, HA Lingxia, JIA Shaotong, LIU Chunlian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  27-32.  doi:10.6040/j.issn.1671-7554.0.2014.561

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Objective To explore the development and fertility potential of oocytes retrieved from surviving prepubertal mice ovarian grafts treated with orthotopic transplantation after a modified solution cryopreservation by vitrification. Methods Prepubertal mice ovaries were cryopreserved by vitrification using ED20 and modified EG5.5/30 solutions. Part of the fresh and vitrified-warmed ovaries were treated with enzyme digestion to test the survival rate of eggs with fluorescence dynamic method. The fluorescence intensity quantitative analysis of microfilament and microtubules of GV oocytes were evaluated with laser confocal microscopy. Part of fresh and vitrified-warmed ovaries were allotransplanted under the ovarian capsule of ovariectomized adult female mice recipients. All juvenile mice were randomly divided into six groups: fresh group and fresh orthotopic transplantation group; ED20 solution vitrified-warmed group and ED20 solution vitrified-warmed orthotopic transplantation group; modified EG5.5/30 solution vitrified-warmed group and modified EG5.5/30 solution vitrified-warmed orthotopic transplantation group. To evaluate the fertility and developmental potential of the fresh and vitrified-warmed surviving ovarian grafts, 15 days after orthotopic transplantation, female micerecipients that restored estrous cycle were mated with male mice and serum levels of estradiol (E₂) in female mice recipients was measured. Results Vitrified-warmed prepubertal mice ovaries showed a high survival rate of oocytes although a remarkable reduction from 92.6% (fresh group) to 87.0% (ED20 solution vitrified-warmed group) and 84.6% (EG5.5/30 solution vitrified-warmed group) was observed respectively (P<0.05). Results of quantitative analysis of microfilament and microtubules of GV oocytes decreased significantly in the two cryopreserved groups when compared with those of fresh group (P<0.05). Female mice recipients that received vitrified-warmed juvenile ovaries could successfully give birth after mating. The first litter time, litter size and total litter size of the two vitrified-warmed orthotopic transplantation groups showed marked differences compared with fresh orthotopic transplantation group (P<0.05). Conclusion Juvenile mice ovaries treated with cryopreservation by vitrification in the modified EG5.5/30 solution and allotransplantation do not affect the development of eggs and reproductive potential, similar in ED20 solution. Female mice recipients can grive normal offspring, but reproductive reserve and fertility significantly decreased.

Dynamics of fasting glucagon and fasting glucagon /insulin ratio with diabetes duration in patients with type 2 diabetes

TIAN Meng, WU Chuanlong, QI Lin, LIU Yan, WANG Chuan, ZHAO Ruxing, LIANG Kai, SONG Jun, HOU Xinguo, CHEN Li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  33-37.  doi:10.6040/j.issn.1671-7554.0.2014.194

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Objective To analyze the dynamics of fasting glucagon (FGLC) and glucagon /insulin (FGLC/FINS) ratio with diabetes durations and their correlations with glycemic control in patients with type 2 diabetes. Methods Two hundred and sixty-two patients with type 2 diabetes were enrolled and divided into five groups according to diabetes duration: <5 years (group A, n=65); ≥ 5 years and <10 years (group B, n=62); ≥10 years and <15 years (group C, n=46); ≥15 years and <20 years (group D, n=45);≥20 years (group E, n=44). FGLC, fasting insulin (FINS), FGLC/FINS ratio, C peptide, HOMA-IR, HOMA-β and other metabolic indicators were measured to explore their correlations with diabetes duration, fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c). Results FGLC levels of groups A, B and C increased progressively with diabetes duration(r=0.243, P<0.05). Oppositely, FGLC levels of groups D and E were lower than that of group C (P<0.05) and were not correlated with diabetes duration. FGLC/FINS ratio was positively correlated with diabetes duration (r=0.243, P<0.05). Multiple stepwise regression analysis showed that the FGLC/FINS ratio was positively correlated with FBG and HbA1c (t=4.474 or 2.212, P<0.05). Conclusion FGLC of patients with type 2 diabetes increased with diabetes duration forthe first 15 years since being diagnosed, then slightly decreased, whereas the FGLC/FINS ratio consistently increased with diabetes duration. The FGLC/FINS ratio might significantly affect the glycemic control in patients with type 2 diabetes.

Predictive value of umbilical cord blood in combination with peripheral artery blood pH for neonatal hypoxic ischemia encephalopathy

SHAO Qin, DIAO Yuqiao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  38-40.  doi:10.6040/j.issn.1671-7554.0.2014.372

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Objective To explore the predictive value of pH in umbilical blood and peripheral arterial blood for the occurring of neonatal hypoxic-ischemic encephalopathy(HIE). Methods pH value of umbilical arterial blood was measured in 917 cases of full-term neonates, sampled during June 2011 to June 2013, before the first cry. Blood gas was analyzed with peripheral arterial blood, sampled at 1 hour after born, from 138 cases whose Apgar scores were less or equal to 7. The effectiveness of HIE detection was compared among umbilical blood, peripheral arterial blood and their combination. Results When umbilical arterial blood pH<7.10 was used as an indicator for HIE diagnosis, the positive prediction value was 55.38%, sensitivity was 60.00% and specificity was 96.61%. When pH value of umbilical arterial blood and arterial blood was used together as the indicator for HIE, the positive prediction value was 95.74%, sensitivity was 88.26% and specificity was 97.10%. Conclusion pH value of peripheral arterial blood is suggested to be determined for new borns whose umbilical arterial blood pH was less than 7.10. The combination of the two indexes can be used to objectively evaluate HIE.

Characteristics of gastric polyps and correlation with helicobacter pylori infection

YANG Xue, QIU Chunhua, LEI Lei, HU Xiao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  41-44.  doi:10.6040/j.issn.1671-7554.0.2014.274

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Objective To analyze the clinical, gastroscopic and pathological characteristics of gastric polyps (GPs) and their correlation with helicobacter pylori infection (Hp). Methods A total of 440 GPs cases confirmed by endoscope and biopsy were recruited. The differences in age, site, size and Hp status of GPs according to various pathological types and sex were analyzed. Results The incidence of GPs increased with age. Fundic gland polyps (FGPs) were more likely to be found in female. The number of FGPs, hyperplastic polyps (HPs), inflammatory polyps (IPs) and adenomatous polyps (APs) was 274, 138, 26, and 2, respectively. The number of polyps in cardia, fundus, body, antrum and pylorus was 22, 234, 106, 75 and 3, respectively. The number of polyps <1 cm, 1-1.9 cm, and ≥2 cm was 392, 40 and 8, respectively. A total of 372 GPs were checked for Hp with tissue slice dyeing. FGPs, HPs, IPs and APs cases with Hp positive was 0, 57, 9, and 1, respectively. There was difference in size of GPs according to various sites (P<0.05). There were differences in site, size and Hp status of GPs according to various pathological types (P<0.05). Conclusion The spectrum of GPs pathology changes. HPs correlates with Hp positively; however, FGPs correlates with Hp negatively. Characteristics of gastric polyps are helpful for preliminary judgment of GPs during gastroscopy, guidance of therapy and follow-up.

Short-term efficacy of tirofiban combined with IABP for patients with complex coronary artery disease after PCI

LIU Yi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  45-48.  doi:10.6040/j.issn.1671-7554.0.2014.223

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Objective To explore the efficacy and prognosis of tirofiban combined with intra aortic balloon pump (IABP) for patients who had complex coronary artery disease after undergoing percutaneous coronary intervention (PCI). Methods Data of 90 cases of complex coronary artery disease confirmed by coronary angiography who underwent PCI during Jan. 2011 to Dec. 2013 in our hospital were retrospectively analyzed. Patients were divided into three groups: tirofiban group (Group A), tirofiban combined with IABP group (Group B), and IABP group (Group C). The vascular lesions, postoperative cardiac function index, bleeding complications and incidence of thrombocytopenia, and incidence of heart disease 30 days after PCI were compared. Results Five days after PCI, left ventricular ejection time (LVEF) in groups B and C were higher than than that in group A (P<0.05), and the level of BNP was significantly higher in Group A than in groups B and C (P<0.05). Platelet counts showed no significant differences among the three groups (P >0.05). One patient in group B and 2 in group C developed thrombocytopenia, but 3 days after stopping using heparin and withdrawing IABP, the platelet count returned to normal. The incidence of major adverse cardiovascular events (MACE) was significantly lower in group B than in groups A and C. Conclusion During PCI, combining tirofiban and IABP early for patients who had complex lesions can significantly improve coronary blood flowand myocardial perfusion, and reduce the incidence of MACE within 30d without increasing the risk of bleeding.

Value of 3D-digital subtraction angiography in the differential diagnosis of vascular lesion originating from the posterior communicating artery

YANG Xiancun, GE Yanyan, LIU Qiang, YU Binchao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  49-54.  doi:10.6040/j.issn.1671-7554.0.2014.161

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Objective To explore the applicative value of three-dimensional digital subtraction angiography (3D-DSA) in diagnosing aneurysm and infundibular dilation (ID) originating from the posterior communicating artery. Methods A total of 47 patients with vascular lesions originating from the posterior communicating artery were analyzed retrospectively. All patients had undergone angiography with both conventional DSA (2D-DSA) and 3D-DSA, and the results were analyzed by two doctors. Results Altogether 26 aneurysms and 6 IDs were found by 2D-DSA, while 33 aneurysms and 14 IDs were detected by 3D-DSA, with a statistical significance between the detection rates (χ²=19.03, P<0.05). There was a statistical difference in displaying the relationship between the lesion and adjacent vessels (Z=-3.62, P<0.05). Conclusion The 3D-DSA can clearly display the relationship between the lesion and adjacent vessels, which is important in the differential diagnosis of ID and aneurysm, thus it is worthy of spreading.

Clinical observation of recombinant human endostatin combined with gemcitabine in the treatment of platinum-resistant recurrent ovarian cancer

ZHANG Miaoci, LI Ping, HA Minwin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  55-59.  doi:10.6040/j.issn.1671-7554.0.2014.347

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Objective To evaluate the efficacy and safety of recombinant human endostatin(endostar) in combination with gemcitabine in the treatment of platinum-resistant recurrent ovarian cancer. Methods A total of 64 patients with platinum-resistant recurrent ovarian cancer were randomly divided into the experimental group (n=33) and control group (n=31). The experimental group received endostar plus gemcitabine while the control group gemcitabine regimen alone. The efficacy, adverse reactions and quality of life were compared. Results Statistically significant differences were observed in the objective response rate, disease control rate and median progression-free survival between the experimental group and control group (39.3% vs 16.1%,75.8% vs 41.9%, 6.5 months vs 3.1 months, P<0.05). No statistically significant differences were found in the median overall survival (13.7 vs 10.9 months), adverse reactions and quality of life between the two groups (P>0.05). Conclusion Endostar in combination with gemcitabine significantly improves short-term effect and median progression-free survival in patients with platinum-resistant recurrent ovarian cancer without increasing the incidence of adverse effects.

Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro

ZHUANG Yong, LI Dong, FU Jinqiu, SHI Qing, JU Xiuli

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  60-64.  doi:10.6040/j.issn.1671-7554.0.2014.303

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Objective To induce and culture dendritic cells (DC) derived from normal children and childhood B lineage acute lymphoblastic leukemia (B-ALL) patients, and compare their biological features in vitro. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 10 B-ALL patients (ALL group) and 10 healthy donors (control group). The isolated PBMCs were co-cultured with rhGM-CSF(20 ng/mL), rh IL-4(10 ng/mL) and TNF-α(10 ng/mL) for 8 days. The morphological features were observed by inverted microscope. CD11c, CD80, CD83, CDD86 expressions were assayed by flow cytometry. The concentration of IL-12 was measured by ELISA. The antigen presenting function of the cells were tested by mixed lymphocyte reaction(MLR). Electrochemical measurement was used for the detection of glucose metabolism. Results The typical DC morphological features were not observed from the cells in ALL group. The cells in ALL group expressed lower levels of CD11c, CD83, CD80 and CD86 than the cells in control group(P<0.05). The cells in ALL group secreted lower level of IL-12 than control group(P<0.05). The cells in ALL group displayed weaker activity of stimulating T lymphocyte proliferation than control group in MLR test. Glucose concentration in culture medium of ALL group was higher than that of control group(P<0.05). Conclusion DC derived from B-ALL patients displays a less mature phenotype with compromised functions. Low glucose metabolism ability might be a possible reason for the DC defects in ALL.

Evaluation of ALS assay (TB-SA antibody) in diagnosis of tuberculous pleurisy

JIAO Jin, WANG Maoshui, WU Yanhua, WANG Xinfeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  65-67.  doi:10.6040/j.issn.1671-7554.0.2014.336

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Objective To investigate the diagnostic value of the ALS assay (TB-SA antibody) in the tuberculous pleurisy. Methods 55 patients diagnosed as tuberculous pleurisy (TPE group) and 45 healthy persons (healthy group) were enrolled. The levels of TB-SA antibody from lymphocyte secretion were detected by ELISA assay. Mann-Whitney U test was used to compare the levels of TB-SA antibody between the two groups. Receiver operating characteristic (ROC) analysis was performed to analyze the sensitivity of ALS assay (TB-SA antibody) in diagnosis of tuberculous pleurisy. Results The mean concetration of TB-SA antibody from lymphocyte secretion in TPE group was significantly higher than that in healthy group (P<0.01). The area under the curve, best cutoff point, sensitivity, specificity, positive likehood ratio and negtive likehood ratio of the ALS assay (TB-SA) were 0.900 (95%CI: 0.824-0.951), 0.04, 77.8%, 92.7%, 10.69 and 0.24, respectively. Conclusion ALS (TB-SA antibody) assay performs well in the diagnosis of tuberculous pleurisy.

Clinical research of cryotherapy and balloon dilation for severe benign central airway stenosis through laryngeal mask

SHI Xiaowu, ZHAO Su

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  68-72.  doi:10.6040/j.issn.1671-7554.0.2014.108

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Objective To observe the short- and long-term efficacy and safety of cryotherapy and balloon dilation for severe benign central airway stenosis through laryngeal mask. Methods Forty-five patients with severe benign central airway stenosis were envolved from January 2011 to December 2013. The patients were treated with cryotherapy and balloon dilation under general anesthesia through laryngeal mask. The airway diameter, forced expiratory volume in one second (FEV1), Hugh-Jones classification, partial pressure of oxygen (PaO₂) and quality of life scores were compared before and after treatment. The short- and long-term efficacy and complications were followed up. Results After three to eigth courses of treatment, airway diameter, FEV1, Hugh-Jones classification, PaO₂ and quality of life scores were improved significantly (P<0.05). Thirty-eight patients (84.4%) relieved well. Their tracheal diameters reached more than 50% of the original ones and the corresponding lung lobes reexpaned completely. The lung lobes of 7 patients (15.6%) reexpanded partly. Recent adverse reactions were sore throat and cough (11 cases, 22.4%), bloody sputum (17 cases, 37.8%), and pneumothorax (2 cases, 4.4%). With the follow-up for 18 months, restenosis occurred in 5 patients (11.1%, 2.5 months averagely). After the treatment of cryotherapy and balloon dilation, the symptoms relieved. Conclusion Cryotherapy and balloon dilation for severe benign central airway stenosis through laryngeal mask is effect and safe, worthy to be promoted clinically.

Therapeutic effect of percutaneous kyphoplasty on osteoporotic multi-compressive fracture

FAN Qingkai, GONG Weiming, NING Bin, SONG Hongliang, WEI Jianlu, JIA Tanghong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  73-76.  doi:10.6040/j.issn.1671-7554.0.2014.304

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Objective To observe and evaluate the therapeutic effect of percutaneous kyphoplasty (PKP) on osteoporotic multi-compressive fracture (OVCF). Methods A retrospective study was conducted on 68 OVCF cases during Jan. 2009 to Dec. 2013. The control group (n=17, 9 vertebrae) was treated with strict-in-bed method, while the surgical group (n=51, 121 vertebrae) was treated with PKP. In addition, the two groups received the same anti-osteoporosis treatment. The clinical and radiological parameters of the two groups were compared, including Barthel index, Cobb angle, visual analogue scale/score (VAS) and Oswesty disability index (ODI). Results After a follow-up of 12～24 months (average 18.6 months), the data indicated that the pain of 51 cases was significantly released or even eradicated 24 hours after surgery. X-ray images showed no obvious bone loss or severe complications. There was significant statistic difference (P<0.05) in Barthel Index, Cobb angle, VAS and ODI in the surgical group before and 12 months after the PKP surgery. There was marked difference (P<0.05) between the surgical group and control group in Cobb Angle one year after PKP surgery (P<0.05). Conclusion PKP is safe and effective to treat osteoporotic multi-Compressive fracture.

Effect of LMWH on severe preeclampsia complicated with FGR and HO-1 expression

DUAN Yan, LIU Yuanyuan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  77-80.  doi:10.6040/j.issn.1671-7554.0.2014.281

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Objective To investigate the effect of low molecular weight heparin (LMWH) on severe preeclampsia complicated with fetal growth restriction (FGR) and expression of hemeoxygenase-1(HO-1). Methods A total of 97 cases were randomized into LMWH group (n=44) and control group (n=53). After 7-day treatment, changes of placental weight were observed. Villous stromal cells, fibrinoid effusion and necrosis, decrease, obstruction and infarction of villus blood vessels were observed with HE staining. HO-1 expression was detected with immunohistochemical method. Results The placental weight increased significantly in LMWH group (P<0.05). Hypoxic-ischemia changes of placenta decreased markedly in LMWH group (P<0.05). The HO-1 protein expression increased remarkably in LMWH group (P<0.05). Conclusion LMWH can improve villi morphology and ischemia hypoxia of placenta for severe preeclampsia complicated with FGR. Additionally, it can increase the expression of HO-1, dilate vessels, and protect the placenta from being oxidized, thus promoting the fetal growth.

Parenting stress effects on mental health: mediating and moderating of coping style and family function

JIANG Hong, XIE Jingtang, DING Juan, LEI Zhen, PAN Fang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  81-85.  doi:10.6040/j.issn.1671-7554.0.2014.266

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Objective To observe the effect of parenting stress, family function and coping style on mental health of parents of adolescents. Methods A total of 200 parents of 12-year-old junior school students in one urban high school were included in the study. All subjects were tested using parenting stress index-short form, simplified coping style questionnaire, family assessment device and symptom check list 90 (SCL-90). Results Female had higher SCL-90 total score than male. Total SCL-90 score was positively related to parenting stress and negative coping (P<0.01), while negatively related to positive coping (P<0.01). Problem-solving and affective involvement were positively related to positive coping (P<0.01). Roles, affective responsiveness, affective involvement and behavioral control were negatively related to negative coping (P<0.05). Coping styles were significant predictors of SCL-90 (P<0.001; P<0.005). Both positive and negative coping had part of mediating role (P<0.001; P<0.005) in parenting stress and mental health. Conclusion Coping style is a part mediator in parenting stress affecting mental health. Problem-solving and affective involvement positively influence positive coping. Role, affective response, affective involvement and behavioral control negatively influence negative coping.

Ecological executive function in adolescent relatives of schizophrenia patients and its relationship with victimization

LI Yang, CAO Fenglin, ZHONG Gengkun, LIN Pingzhen, LIANG Xinhao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  86-91.  doi:10.6040/j.issn.1671-7554.0.2014.298

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Objective To investigate specific executive deficits in the real-life environment among adolescent relatives of schizophrenia patients and to explore the effect of specific victimization types on the deficits. Methods The study included 104 non-affected adolescent descendants and siblings of schizophrenia patients in the relative group and 193 healthy adolescents in the healthy control group, all aged between 9 and 20 years. All participants completed the Behavior Rating Inventory of Executive Function-Self Report Version (BRIEF-SR) and Juvenile Victimization Questionnaire, Child Self-report Version (JVQ). Results The multivariate analysis revealed that the relative group reported more difficulties on the shift and task completion scale than controls (P<0.05). Significant main effects were detected for conventional crime on the plan/organize and task completion scale (P<0.05) and for child maltreatment on the inhibit, emotional control, monitor, working memory and organization of material scale (P<0.05). The univariate analysis revealed that in the relative group, adolescents who had experienced conventional crime reported higher scores on the plan/organize and task completion scale compared with those without conventional crime (P<0.05); adolescents with child maltreatment had higher scores on the Inhibit, emotional control, and organization of material scale than those without child maltreatment (P<0.05). For the healthy controls, adolescents with child maltreatment showed poorer scores on the Working Memory scale than those without maltreatment (P<0.05). Conclusion Our resultssuggest certain impaired aspects of executive functioning in real-life situations among adolescent relatives of schizophrenia patients. Conventional crime and child maltreatment may be associated with executive dysfunction in the adolescent relatives.

Genome-wide association study of autism in Shandong Province

YANG Shulin, YANG Fan, ZHAI Jing, LIU Jintong, ZHANG Yan, CHEN Gang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  92-96.  doi:10.6040/j.issn.1671-7554.0.2014.554

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Objective To investigate the genetic loci associated with autism in the population of Shandong Province. Methods Linkage Mapping Set 2.5 including 400 microsatellite markers was used to screen 3 separated DNA pooling samples consisting of 38 autism children, their 75 parents and 1 000 normal controls. CLUMP and SPSS12.0 software were adopted for statistic analysis to compare the difference in allele frequency of each locus between autism children and controls, autism children and parents respectively. Results Significant differences in allele frequency were found at 1p36.31, 4q13.3, 4q35.1 and 7p21.3. Conclusion Genetic loci 1p36.31, 4q13.3, 4q35.1 and 7p21.3 are detected to be associated with autism in the population of Shandong Province, and susceptibility genes may be located around these regions.

Identification and sequence analysis of human enteroviruses isolated from a polluted river in Jinan City, Shandong Province, 2011

TAO Zexin, YANG Jing, WANG Haiyan, LIN Xiaojuan, WANG Suting, SONG Lizhi, XU Aiqiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  97-100.  doi:10.6040/j.issn.1671-7554.0.2014.418

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Objective To investigate the human enteroviruses (HEVs) in Xiluo River which flows through the metropolitan area of Jinan City, and to analyze the genetic characterization of the isolates. Methods Water samples were collected monthly from the river during May to July, 2011. The specimens were concentrated by anionic membrane absorption and elution, and inoculated on RD, HEp-2, and L20b cell lines. The VP1 coding regions of the isolates were sequenced and phylogeneticly analyzed. Results Altogether 9 HEVs, 4 echovirus 6 (E6) and 5 coxsackievirus B2 (CVB2), were isolated. VP1 sequence analysis revealed the river E6 had high homology (95.9%-96.7%) with the aseptic meningitis isolate found in Linyi City in 2010, suggesting the local occurrence of E6 was associated with aseptic meningitis. River E6 also had high homology (94.5%-99.7%) with prevalent E6 strains in Jinan City in 2010. The 5 CVB2 strains were highly homologous (98.4%-100%), which had 80.9%-95.2% homology with isolates from previous acute flaccid paralysis cases in Shandong Province. Conclusion The domestic sewage-polluted river can serve as sampling site for HEV environmental surveillance, which offers an effective approach to comprehend the local HEV circulation in the context of no specialized surveillance system in China.

Capillary culture method using modified semisolid Rappaport-Vassiladis medium for Salmonella detection in poultry

LIU Hui, HU Guangchun, SHI Yuwen, LI Jian, LIU Lanzheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  101-105.  doi:10.6040/j.issn.1671-7554.1.2014.011

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Objective To develop capillary culture method using modified semisolid Rappaport-Vassiladis (MSRV) medium (referred to as modified culture method) which was introduced for preliminary screening of Salmonella in poultry products. Methods Capillary tube loading MSRV medium was inserted into liquid medium (enrichment medium or preenrichment medium) to screen Salmonella in samples. The sensitivity and specificity of the modified culture method were evaluated in 129 Salmonella strains and 58 non-Salmonella strains. The detectability of the modified culture method was assessed using poultry samples, referred to ISO 6579:2002 microbiology of food and animal feeding stuffs—horizontal method for the detection of Salmonella spp. Results The sensitivity of the modified culture method was 96.9% (125/129) for Salmonella strains, and the specificity was 96.6% (56/58) for non-Salmonella strains. The modified culture method which detected 33.3% (20/60) of poultry samples was not statistically different from ISO 6579:2002 that detected 35.0% (21/60) of poultry samples (P >0.05). Compared with ISO 6579:2002, 91.7% (33/36), negative samples did not need further isolation and identification after using the modified culture method; samples were early screened in 36 h that was half of the original time. Conclusion The detectability of the modified culture method for poultry products is not significantly different from ISO 6579:2002. The advantage of the modified culture method is saving labor, resources and time.

Genetic polymorphisms of ADIPOQ and CAPN-10 and colorectal cancer risk

LIU Wenhui, LI Xiaoling, YUAN Ping, LUAN Rongsheng, FENG Fei, HU Xiaoqin, YAN Jin, YANG Yanfang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(11):  106-112.  doi:10.6040/j.issn.1671-7554.0.2014.449

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Objective To explore the associations of ADIPOQ (rs2241766, rs1501299) and CAPN-10 (rs3842570, rs3792267) polymorphisms and gene-gene interaction with the risk of colorectal cancer(CRC). Methods A 1:1 matched case-control study was conducted to collect the demographic features, lifestyle, and dietary habits of 400 patients with newly histopathologically diagnosed CRC and 400 matched health controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of ADIPOQ (rs2241766, rs1501299) and CAPN-10 (rs3842570, rs3792267). Conditional logistic regression was used to analyze the association of genetic polymorphisms of the 4 SNPs and CRC risk. Generalized multifactor dimensionality reduction (GMDR) was applied to test the gene-gene interaction on CRC risk. Results Compared with the wild genotypes, subjects carryingADIPOQ rs2241766 TG+GG genotype and CAPN-10 rs3792267 GA+AA genotype had the increased CRC risk (rs2241766: OR=1.354, 95%CI: 1.004-1.827; rs3792267: OR=1.507, 95%CI: 1.009-2.250), while those carrying ADIPOQ rs1501299 GT+TT genotype had an decreased CRC risk (OR=0.680, 95%CI:0.501-0.923). The association between CAPN-10 rs3842570 Del/Ins+Ins/Ins genotype and CRC risk was not significant (OR=1.345, 95%CI: 0.849-2.130). The GMDR showed that the gene-gene interaction among the 4 locus on CRC risk was significant (P=0.001). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (χ²=13.302, P<0.001). Conclusion ADIPOQ rs2241766, ADIPOQ rs1501299, and CAPN-10 rs3792267 are associated with the risk of CRC, and the 4 locus might affect the risk of CRC together.