JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 97-100.doi: 10.6040/j.issn.1671-7554.0.2014.418

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Identification and sequence analysis of human enteroviruses isolated from a polluted river in Jinan City, Shandong Province, 2011

TAO Zexin1, YANG Jing2, WANG Haiyan1, LIN Xiaojuan1, WANG Suting1, SONG Lizhi1, XU Aiqiang1   

  1. 1. Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan 250014, Shandong, China;
    2. Jining Medical College, Jining 272100, Shandong, China
  • Received:2014-06-24 Published:2014-11-10

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Abstract: Objective To investigate the human enteroviruses (HEVs) in Xiluo River which flows through the metropolitan area of Jinan City, and to analyze the genetic characterization of the isolates. Methods Water samples were collected monthly from the river during May to July, 2011. The specimens were concentrated by anionic membrane absorption and elution, and inoculated on RD, HEp-2, and L20b cell lines. The VP1 coding regions of the isolates were sequenced and phylogeneticly analyzed. Results Altogether 9 HEVs, 4 echovirus 6 (E6) and 5 coxsackievirus B2 (CVB2), were isolated. VP1 sequence analysis revealed the river E6 had high homology (95.9%-96.7%) with the aseptic meningitis isolate found in Linyi City in 2010, suggesting the local occurrence of E6 was associated with aseptic meningitis. River E6 also had high homology (94.5%-99.7%) with prevalent E6 strains in Jinan City in 2010. The 5 CVB2 strains were highly homologous (98.4%-100%), which had 80.9%-95.2% homology with isolates from previous acute flaccid paralysis cases in Shandong Province. Conclusion The domestic sewage-polluted river can serve as sampling site for HEV environmental surveillance, which offers an effective approach to comprehend the local HEV circulation in the context of no specialized surveillance system in China.

Key words: Enterovirus, Environmental monitoring, Coxsackievirus infections, Phylogeny, Echovirus 6, human

CLC Number: 

  • R373
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