JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 16-19.doi: 10.6040/j.issn.1671-7554.0.2015.227

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Cell injury induced by 3CD protein of Enterovirus 71

LI Jing, QIAO Qiao, SUN Lele, MA Yingwei, ZHUANG Zhichao, ZHAO Li, WANG Zhiyu, WEN Hongling   

  1. Department of Virology, School of Public Health, Key Laboratory for Experimental Teratology of the Ministry of Education, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-03-03 Online:2015-12-10 Published:2015-12-10

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Abstract: Objective To investigate the roles of 3CD protein on pathogenesis of Enterovirus 71(EV71). Methods The cells were divided into control group, SDLY1 group, SDLY107 group, and SDLY107(1-3CD) group. 3CD region of SDLY107, which was isolated from the severe case, was replaced by that of SDLY1, which was isolated from the mild case, through reverse genetics, and the recombinant virus was named as SDLY107(1-3CD). EV71 strains were incubated at 37℃ and 39.5℃. After some time, the living cells were detected by MTT assay to show cell injury induced by different strains. Results Compared with SDLY107, cell injury induced by SDLY107(1-3CD) decreased at 37℃ and 39.5℃ (both P<0.05). There was statistical difference between cell injury induced by SDLY1 and SDLY107(1-3CD) at 37℃ (P<0.05), while there was no statistical difference between cell injury induced by SDLY1 and SDLY107(1-3CD) at 39.5℃(P>0.05). Conclusion Replacement of EV713CD can change cell injury induced by EV71, and there may be virulence determinants on 3CD region, which may help us understand the pathogenesis of EV71.

Key words: 3CD protein, Cell injury, Enterovirus 71

CLC Number: 

  • R373.2
[1] Mao LX, Wu B, Bao WX, et al. Epidemiology of hand, foot, and mouth disease and genotype characterization of Enterovirus 71 in Jiangsu, China[J]. J Clin Virol, 2010, 49(2):100-104.
[2] Zhang SB, Liao H, Huang CH, et al. Serum types of enterovirus and clinical characteristics of 237 children with hand, foot and mouth disease in Shenzhen[J]. CJCP, 2008, 10(1):38-41.
[3] McMinn P, Lindsay K, Perera D, et al. Phylogenetic analysis of enterovirus 71 strains isolated during linked epidemics in Malaysia, Singapore, and Western Australia[J]. J Virol, 2001, 75(16):7732-7738.
[4] Kehle J, Roth B, Metzger C, et al. Molecular characterization of an Enterovirus 71 causing neurological disease in Germany[J]. J Neurovirol, 2003, 9(1):126-128.
[5] McMinn PC. An overview of the evolution of enterovirus 71 and its clinical and public health significance[J]. FEMS Microbiol Rev, 2002, 26(1):91-107.
[6] Wen HL, Si LY, Yuan XJ, et al. Complete genome sequencing and analysis of six enterovirus 71 strains with different clinical phenotypes[J]. Virol J, 2013, 10(1):115-125.
[7] 袁晓晶,温红玲,高峰,等.肠道病毒71型基因组5'非编码区序列分析[J].山东大学学报:医学版, 2012, 50(9):127-132. YUAN Xiaojing, WEN Hongling, GAO Feng, et al. Sequence analysis for 5' untranslated region of genomes from enterovirus 71[J]. Journal of Shandong University:Health Sciences, 2012, 50(9):127-132.
[8] 温红玲,郝树彬,高峰,等.肠道病毒71型山东临沂分离株全基因组序列分析[J].中华微生物学和免疫学杂志, 2011, 31(7):603-608. WEN Hongling, HAO Shubin, GAO Feng, et al. Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi,Shandong Province[J]. Chinese Journal of Microbiology and Immunology, 2011, 31(7):603-608.
[9] Sun LL, Wang JK, Cui XQ, et al. Association of viral replication capacity with the pathogenicity of enterovirus 71[J]. Virus Res, 2014, 189(5):1-7.
[10] 吴家兵,方益荣,王建军,等.安徽省2008年手足口病流行病学分析[J].安徽预防医学杂志, 2010, 16(2):96-98. WU Jiabing, FANG Yirong, WANG Jianjun, et al. Epidemiological analysis of hand, foot and mouth disease in Anhui Province in 2008[J]. Anhui Journal of Preventive Medicine, 2010, 16(2):96-98.
[11] 刘威龙,韦清,杨桂林,等.深圳5例肠道病毒71型手足口病病毒株全基因组测序分析[J].中华微生物学和免疫学杂志, 2010, 30(5):409-409. LIU Weilong, WEI Qing, YANG Guilin, et al. Complete genome sequencing and analysis of five enterovirus 71 strains in Shenzhen[J]. Chinese Journal of Microbiology and Immunology, 2010, 30(5):409-409.
[12] Barco A, Feduchi E, Carrasco L. Poliovirus protease 3C(pro) kills cells by apoptosis[J]. Virology, 2000, 266(2):352-360.
[13] Tardy-Panit M, Blondel B, Martin A, et al. A mutation in the RNA polymerase of poliovirus type 1 contributes to attenuation in mice[J]. J Virol, 1993, 67(8):4630-4638.
[14] Kung YH, Huang SW, Kuo PH, et al. Introduction of a strong temperature-sensitive phenotype into enterovirus 71 by altering an amino acid of virus 3D polymerase[J]. Virology, 2010, 396(1):1-9.
[15] Clark ME, Lieberman PM, Berk AJ, et al. Direct cleavage of human TATA-binding protein by poliovirus protease 3C in vivo and in vitro[J]. Mol Cell Biol, 1993, 13(2):1232-1237.
[16] Weidman MK, Yalamanchili P, Ng B, et al. Poliovirus 3C protease-mediated degradation of transcriptional activator p53 requires a cellular activity[J]. Virology, 2001, 291(2):260-271.
[17] Falk MM, Grigera PR, Bergmann IE, et al. Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3[J]. J Virol, 1990, 64(2):748-756.
[18] Clark ME, Hämmerle T, Wimmer E, et al. Poliovirus proteinase 3C converts an active form of transcription factor ⅢC to an inactive form:a mechanism for inhibition of host cell polymerase Ⅲ transcription by poliovirus[J]. EMBO J, 1991, 10(10):2941-2947.
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