JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 16-19.doi: 10.6040/j.issn.1671-7554.0.2015.227

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Cell injury induced by 3CD protein of Enterovirus 71

LI Jing, QIAO Qiao, SUN Lele, MA Yingwei, ZHUANG Zhichao, ZHAO Li, WANG Zhiyu, WEN Hongling   

  1. Department of Virology, School of Public Health, Key Laboratory for Experimental Teratology of the Ministry of Education, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-03-03 Online:2015-12-10 Published:2015-12-10

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Abstract: Objective To investigate the roles of 3CD protein on pathogenesis of Enterovirus 71(EV71). Methods The cells were divided into control group, SDLY1 group, SDLY107 group, and SDLY107(1-3CD) group. 3CD region of SDLY107, which was isolated from the severe case, was replaced by that of SDLY1, which was isolated from the mild case, through reverse genetics, and the recombinant virus was named as SDLY107(1-3CD). EV71 strains were incubated at 37℃ and 39.5℃. After some time, the living cells were detected by MTT assay to show cell injury induced by different strains. Results Compared with SDLY107, cell injury induced by SDLY107(1-3CD) decreased at 37℃ and 39.5℃ (both P<0.05). There was statistical difference between cell injury induced by SDLY1 and SDLY107(1-3CD) at 37℃ (P<0.05), while there was no statistical difference between cell injury induced by SDLY1 and SDLY107(1-3CD) at 39.5℃(P>0.05). Conclusion Replacement of EV713CD can change cell injury induced by EV71, and there may be virulence determinants on 3CD region, which may help us understand the pathogenesis of EV71.

Key words: 3CD protein, Cell injury, Enterovirus 71

CLC Number: 

  • R373.2
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