JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 12-15,32.doi: 10.6040/j.issn.1671-7554.0.2015.131

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An in vitro study of the anticancer effect of salidroside on human gastric carcinoma SGC-7901 cells

WEI Lilan, MO Shurong   

  1. Department of Physiology, Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2015-02-02 Online:2015-12-10 Published:2015-12-10

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Abstract: Objective To investigate the effect of salidroside on the proliferation and growth of in vitro cultured human gastric carcinoma SGC-7901 cells, and to explore the possible anti-tumor mechanism. Methods Cells were divided into control group, 0.50 mg/mL salidroside group, 1.00 mg/mL salidroside group and 2.00 mg/mL salidroside group. Cell proliferation was assessed by MTT array. Argyrophilic nucleolar organiser region proteins (AgNOR) staining was used to determine the expression of AgNOR in nuclear. The cell cycle and apoptosis were detected by flow cytometry test. Migration rate was measured by cell scratch assay. Results Compared with control group, the proliferation and AgNOR expression of SGC-7901 cells were inhibited significantly in salidroside groups (P<0.05) with a concentration-, time-and effect-depent manner; cell circle arrest increased at G2/M phase, but decreased at S phase (P<0.05); the rate of cell apoptosis increased, while cell migration rate was down-regulated (P<0.05). Conclusion Salidroside can effectively inhibit proliferation, arrest cell cycle, induce apoptosis and decrease migration rate of SGC-7901 cell.

Key words: Gastric carcinoma cells, Proliferation, Salidroside, Anti-tumor

CLC Number: 

  • R331
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