JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (9): 32-36.doi: 10.6040/j.issn.1671-7554.0.2015.1255

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Effect of expression of ribosomal protein S6 Kinase 4 variants on the proliferation of breast cancer cell MDA-MB-231

TIAN Siqi, LIU Riqiang, YANG Ning, WEI Wei, YANG Huawei   

  1. Department of Breast Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2015-12-08 Online:2016-09-10 Published:2016-09-10

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Abstract: Objective To investigate the effect of expression of ribosomal protein S6 kinase 4 variants(RSK4m)on the proliferation of breast cancer cell line MDA-MB-231. Methods Lentiviral vector of RSK4 variants were constructed and transfected on breast cancer cell line MDA-MB-231. A negative control group(mock group)and overexpression RSK4m1 group(OE1 group), overexpression RSK4m2 group(OE2 group), overexpression RSK4m3 group(OE3 group)were set respectively. The expressions of three RSK4 variants were detected with qRT- PCR and Western blot. The proliferation of transfected cells was assayed by Cell Counting Kit-8. Finally, the interaction of RSK4m with heat shock proteins observed in preliminary experiments was verified with CO-IP. Results The mRNA and protein expressions of RSK4 variants were significantly up-regulated in transfected cells(all P<0.05). Compared with the mock group, the OE1, OE2 and OE3 groups had significant differences in the inhibition rate(%)at 48, 72, and 96 hour(P<0.05). CO-IP results confirmed the existence of interaction between heat shock protein and RSK4m. Conclusion The expression of RSK4m can inhibit the proliferation of MDA-MB-231. Its regulatory mechanism may differ from that of RSK4w.

Key words: Ribosomal protein S6 Kinase 4, Variant, Breast tumor, Interacting protein, Proliferation

CLC Number: 

  • R739.9
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