JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 6-11.doi: 10.6040/j.issn.1671-7554.0.2016.1591

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Expression changes and mechanism of Nod-like receptor protein 3 inflammasome in the cerebral microvascular endothelial cells in type 2 diabetes mellitus

LI Shuai1, WANG Yalin2, SUN Zhongwen3, ZHU Meijia4   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Tumor Research Institute, Capital Medical University, Beijing 100069, China;
    3. Department of Neurology, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China;
    4. Department of Neurology, Qianfoshan Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-11-30 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To investigate the expression changes and mechanism of Nod-like receptor protein 3(NLRP3)inflammasome in cerebral microvascular endothelial cells of type2 diabetes mellitus. Methods A total of 3-month old Wistar rats(n=5)and spontaneous type 2 diabetic rats(GK, n=5)were enrolled. The expression of NLRP3 in the brain tissues was observed with immunohistochemical method. After cerebral microvascular endothelial cells(CMECs)were cultivated in vitro, they were divided into 5 groups: normal control group, high glucose group 1(HG1 group, glucose concentration=10 mmol/L), high glucose group 2(HG2 group, glucose concentration=20 mmol/L), high 山 东 大 学 学 报 (医 学 版)55卷3期 -李帅,等.Nod样受体蛋白3炎性体在2型糖尿病脑微血管内皮细胞中的变化及变化机制 \=-glucose group 3(HG3 group, glucose concentration=30 mmol/L), and HG+NAC group. The protein expressions of thioredoxin interacting protein(TXNIP)and NLRP3 were detected with Western blotting. The relationship between NLRP3 and TXNIP was analyzed. The content of interleukin-1β(IL-1β)was evaluated with ELISA kits. The content of intracellular reactive oxygen species(ROS)was measured with flow cytometry. Results NLRP3 gathered in microvascular endothelial cells. Compared with Wistar rats, GK rats had increased strength and number of vascular with positive staining(P<0.05). Compared with normal control group, HG1, HG2 and HG3 groups had higher expressions of TXNIP and NLRP3(P<0.01), especially in the HG3 group, in which the level of IL-1β and ROS also increased (P<0.01). TXNIP and NLRP3 was positively correlated(r=0.993; P<0.05). Lompared with HG3 group, in the HG+NAC group, the level of intracellular ROS, TXNIP, NLRP3 and IL-1β decreased(P<0.01). Conclusion NLRP3 inflammasome is activated in type 2 diabetes mellitus by ROS-TXNIP pathway. It promotes the release of inflammatory factor IL-1β and plays an important role in the microvascular injury.

Key words: Type 2 diabetes mellitus, Reactive oxygen species, Nod-like receptor protein 3 inflammasome, Cerebral microvascular endothelial cells, Thioredoxin interacting protein

CLC Number: 

  • R58
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