JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 16-21.doi: 10.6040/j.issn.1671-7554.0.2015.1096

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CXCL16 gene silencing alleviates injury of mouse podocytes treated with the oxidized low-density lipoprotein

WANG Xue1, LI Qian1, WANG Li1, SUN Shuzhen1, MA Aihua2   

  1. 1. Department of Paediatric Nephrology &
    Rheumatism and Immunology, 2. Department of Paediatric Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-11-10 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To investigate the role of chemokine ligand 16(CXCL16)gene silencing on alleviating injury of podocytes treated with oxidized low-density lipoprotein(ox-LDL). Methods The mouse podocytes were transfected with CXCL16 gene short hairpin RNA(shRNA)lentivirus vector and negative control lentivirus vector. The protein and mRNA expressions of CXCL16 were determined with Western blotting and Real-time PCR. The vitality of podocytes was detected with CCK8 assay. The mouse podocytes(cell line)were divided into 4 groups: negative control group(NC group), ox-LDL group, CXCL16-shRNA group, and ox-LDL+CXCL16-shRNA group. The total cholesterol was detected with cholorimetric detection to measure intracelluar lipid accumulations. The expressions of CXCL16 and integrin-β1 were detected with Western blotting. The expression of ROS was detected with ROS Deep Red Dye. Results The inhibition rate of CXCL16 mRNA was about 70%, and that of CXCL16 protein was about 60%, compared to the negative control(both P=0.008). CCK8 revealed no significant difference in the vitality of podocytes between NC group and CXCL16-shRNA group(P=0.712). Compared to the ox-LDL group, in the ox-LDL+CXCL16-shRNA group, the intracelluar lipid accumulations were reduced(P<0.001), integrin-β1 was increased(P=0.004), 山 东 大 学 学 报 (医 学 版)54卷6期 -王雪,等.CXCL16基因沉默减轻ox-LDL对小鼠足细胞损伤 \=-and ROS was reduced(P<0.001). Conclusion CXCL16 gene silencing is likely to play an important role for podocytic protection after the treatment of ox-LDL by reducing the synthesis of ROS and elevating the expression of integrin-β1.

Key words: Chemokine ligand 16, Reactive oxygen species, Integrin-β1, Podocyte, Oxidized low-density lipoprotein, Short hairpin RNA

CLC Number: 

  • R726.9
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