Table of Content

  

20 June 2016
Volume 54 Issue 6

Combination of bone marrow mesenchymal stem cells, platelet gel and extrocorporeal shock wave on bone regeneration

SONG Ke, LIU Huan, WU Wenliang, LIU Haichun, LI Shangzhi, CHEN Yunzhen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  1-6.  doi:10.6040/j.issn.1671-7554.0.2015.1162

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Objective To evaluate whether platelet gel(PG)and extracorporeal shock wave(ESW)could enhance the effect of bone marrow mesenchymal stem cell(BMSCs)in the treatment of nonunion and to select the best therapy on bone regeneration. Methods A total of 125 atrophic nonunion models of rats were established and randomly divided into 5 groups with 25 animals in each group: nonunion group, BMSCs group, BMSCs+PG group, BMSCs+ESW group, and BMSCs+PG+ESW group. Bone regeneration was evaluated via X-ray and micro-CT 4 and 8 weeks after treatment. All rats were sacrificed for histological analysis in week 8. Results X-ray images showed that the ratio of bone union in BMSCs+PG+ESW group was the highest(P<0.05). Micro-CT showed that BMSCs+PG+ESW group contained significantly larger bone volume fraction, thicker and higher number of bone trabecular, and smaller ratio of bone surface / bone volume compared with the other 4 groups(P<0.05). Histomorphometry showed that the BMSCs+PG+ESW group had the largest number of osteoblasts, vascular buds and bone areas fraction among all groups(P<0.05). Conclusion PG and ESW can enhance the effect of BMSCs on bone regeneration. Combination of BMSCs, PG and ESW can produce better results than the combination of two items and monotherapy.

Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds

CHEN Yihui, LIU Xuewei, HOU Xuhao, SUN Qinfeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  7-11.  doi:10.6040/j.issn.1671-7554.0.2015.341

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Objective To observe the osteogenesis and degradation of dialdehyde letilla striata glucomanna(DBsGM)/hydroxypropyl chitosan(HPCS)/nano-nacre powder(NNP)composited scaffolds in rat alveolar bone defect regeneration. Methods A total of 32 male Wistar rats with bilateral mandibular molar area box defects were randomly divided into 4 groups according to the disposal time(week 2, 4, 6, 8), with 8 animals in each group. The DBsGM/HPCS/NNP composited scaffolds were implanted in the experimental side, while nothing was implanted in the control side. The bone defect repair effect was evaluated with hematoxylin-eosin staining and histologic measurement. Results As time extended, the percentage of new bone area increased gradually, and there was significant difference between different time points(P<0.05). At the same time point, the experimental group was superior to the control group in gross observation and histological observation(P<0.05). The implanted materials gradually degraded with time. During week 6 to 8, the implanted materials completely degraded. Conclusion DBsGM/HPCS/NNP composited scaffolds can promote rat alveolar bone defect repair and regeneration.

Down-regulation of α-catulin inhibits the invasion and migration of human tongue squamous cancer cell line Tscca in vitro

MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  12-15.  doi:10.6040/j.issn.1671-7554.0.2015.855

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Objective To explore the effect of α-catulin down-regulation on the invasion and migration of tongue squamous cancer cell line Tscca. Methods Cells were divided into α-catulin-siRNA transfection group, empty plasmid transfection group, and blank group. The α-catulin down-expressing plasmid was constructed and transfected into human tongue squamous cancer cell line Tscca. The protein expression of α-catulin was tested by Western blotting. The in vitro invasion and migration abilities of Tscca cells were measured by Transwell assays and Wound Healing. Results The protein expression of α-catulin reduced remarkably in α-catulin-siRNA trasfection group. Wound-healing and Transwell assays showed that the invasion and migration of Tscca cells were suppressed in α-catulin-siRNA transfection group. Conclusion SiRNA-mediated silencing of the α-catulin gene can significantly inhibit cell invasion and migration in human squamous cell carcinoma line Tscca, indicating that α-catulin might be used as an underlying target for molecular therapy of tongue cancer.

CXCL16 gene silencing alleviates injury of mouse podocytes treated with the oxidized low-density lipoprotein

WANG Xue, LI Qian, WANG Li, SUN Shuzhen, MA Aihua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  16-21.  doi:10.6040/j.issn.1671-7554.0.2015.1096

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Objective To investigate the role of chemokine ligand 16(CXCL16)gene silencing on alleviating injury of podocytes treated with oxidized low-density lipoprotein(ox-LDL). Methods The mouse podocytes were transfected with CXCL16 gene short hairpin RNA(shRNA)lentivirus vector and negative control lentivirus vector. The protein and mRNA expressions of CXCL16 were determined with Western blotting and Real-time PCR. The vitality of podocytes was detected with CCK8 assay. The mouse podocytes(cell line)were divided into 4 groups: negative control group(NC group), ox-LDL group, CXCL16-shRNA group, and ox-LDL+CXCL16-shRNA group. The total cholesterol was detected with cholorimetric detection to measure intracelluar lipid accumulations. The expressions of CXCL16 and integrin-β1 were detected with Western blotting. The expression of ROS was detected with ROS Deep Red Dye. Results The inhibition rate of CXCL16 mRNA was about 70%, and that of CXCL16 protein was about 60%, compared to the negative control(both P=0.008). CCK8 revealed no significant difference in the vitality of podocytes between NC group and CXCL16-shRNA group(P=0.712). Compared to the ox-LDL group, in the ox-LDL+CXCL16-shRNA group, the intracelluar lipid accumulations were reduced(P<0.001), integrin-β1 was increased(P=0.004), 山 东 大 学 学 报 (医 学 版)54卷6期 -王雪,等.CXCL16基因沉默减轻ox-LDL对小鼠足细胞损伤 \=-and ROS was reduced(P<0.001). Conclusion CXCL16 gene silencing is likely to play an important role for podocytic protection after the treatment of ox-LDL by reducing the synthesis of ROS and elevating the expression of integrin-β1.

Effects of inflammatory cytokines secreted by nucleus pulposus cells on proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts

WANG Qingshi, CHEN Yunzhen, LIU Haichun, WU Wenliang, JIAO Guangjun, LI Xiaofeng, XU Daxia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  22-26.  doi:10.6040/j.issn.1671-7554.0.2016.069

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Objective To investigate effects of inflammatory cytokines secreted by nucleus pulposus cells on proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts. Methods Posterior longitudinal ligamen(PLL)cells were harvested by tissue fragment culture. Nucleus pulposus(NP)cells were isolated by enzymatic digestion of nucleus pulposus tissue. The cells were divided into OPLL+NP group, OPLL group and NP group. Secreted cytokines were detected by ELISA. Cell cytotoxicity was measured with MTT assay and cell proliferation was detected with EdU assay. The expressions of types Ⅰ, Ⅺ collagen and osteocalcin mRNA were determined with real time PCR(RT-PCR). The osteogenic activity of OPLL cells was assessed with histochemical stains(Von Kossa and ALP stain). Results In the OPLL group, OD₄₉₀ decreased compared with OPLL+NP group［(0.25±0.06)vs(0.28±0.09), P<0.05］. Cell proliferation in OPLL+NP group was significantly higher than that in OPLL group. OPLL+NP group was strong positive for Von Kossa and alkaline phosphatase, while OPLL group was negative. Expressions of types Ⅰ and Ⅺ collagen and osteocalcin mRNA in OPLL+NP group were higher then those in OPLL group(P<0.05). Supernatant from OPLL+NP group showed increased production of IL-1α, TNF-α and PGE2 compared with OPLL group 山 东 大 学 学 报 (医 学 版)54卷6期 -王庆石,等.髓核细胞分泌的炎症因子对后纵韧带成纤维细胞增殖与成骨能力的影响 \=-and NP group(P<0.05). Conclusion In vitro, inflammatory cytokines secreted by nucleus pulposus cells promote proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts.

Diagnostic value of MRI for brucellar spondylitis

GAO Meng, SUN Jianmin, JIANG Zhensong, CUI Xingang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  27-30.  doi:10.6040/j.issn.1671-7554.0.2015.1342

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Objective To explore the characteristics of Brucellar spondylitis on magnetic resonance image(MRI)for the early diagnosis. Methods The MRI features of 26 patients with Brucellar spondylitis were retrospectively analyzed, including T₁-WI, T₂-WI and fat suppressed phase, the favorite infected segment, different abnormal signal formation and destruction on the infected vertebra and disc, paraspinal abscess formation and the spread range. Results Of all 26 cases, lumbar and sacral spine involvement was observed in 22 cases(85%); thoracic involvement was observed in 4 cases; and no cervical involvement was observed. Skip lesion was observed in 3 cases. Altogether 36 discs and 62 vertebrae were affected. T₁-WI revealed that 50(81%)infected vertebrae and 23(64%)infected discs showed normal signal. T₂-WI revealed that 18(29%)involved vertebrae showed mixed intense signal and 19(53%)infected discs showed hyperintensity. FS-MRI revealed that 45(73%)infected vertebrae and 28(78%)involved discs showed high signal. Half of the infected vertebrae 31(50%)showed focal and fan-shape signal and another half 31(50%)showed diffused signal. An inflammatory reaction line could be observed in 56%(20/36)of the involved discs. Peridiscal bone destruction was observed in 28(45%)of the infected vertebrae. Spinal kyphosis was observed in 2 cases, intraspinal abscess in 12 cases, anterior subligamentous abscess in 12 cases, of which 1 case involved more than 3 vertebrae, 7 cases involved less than 3 vertebrae and 4 cases had no spread of abscess. Conclusion MRI is an effective modality to diagnose Brucellar spondylitis.

Association between PTGER4 gene polymorphism and susceptibility of ankylosing spondylitis in Han population in Shandong Province

WANG Liya, MA Zhenzhen, YANG Qingrui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  31-34.  doi:10.6040/j.issn.1671-7554.0.2016.022

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Objective To investigate the relationship between the single nucleotide polymorphisms(SNPs)in PTGER4 and susceptibility of ankylosing spondylitis(AS)in the Han population in Shandong Province. Methods A total of 600 AS patients and 620 healthy controls were involved in this study. Three SNPs(rs7726237, rs4133101 and rs16870224)of PTGER4 gene were detected with the TaqMan-PCR method. The genotype and allele frequencies were calculated, and the distribution difference between the genotype and allele frequencies of SNPs was analyzed. Results Distribution frequencies of A alleles(78.5%, 74.7%)and G alleles(21.5%, 25.3%)of PTGER4 rs7726237 in AS and controls were significantly different with each other(P=0.026, OR=0.81, 95%CI: 0.67-0.97), while the distribution frequency of genotypes(GG, AG, AA)in group AS and controls were(5.3%, 32.3%, 62.3%)and(5.2%, 40.3%, 54.5%)(P=0.014, OR=0.90, 95%CI: 0.54-1.51 and P=0.014, OR=0.70, 95%CI: 0.55-0.89, respectively). Frequency of alleles and the genotypes of rs4133101 and rs16870224 SNPs between AS and controls group were similar(P>0.05). No association was found between haplotypes of SNPs in PTGER4 and susceptibility of AS(P>0.05). Conclusion This study indicates that PTGER4 gene polymorphism is related to AS susceptibility.

Reconstruction of proximal tibial bone defect during primary total knee arthroplasty and its clinical efficacy

ZHOU Chuibao, MA Liang, LI Ming, ZHANG Yuankai

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  35-38.  doi:10.6040/j.issn.1671-7554.0.2015.809

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Objective To investigate the efficacy of proximal tibial bone defect reconstruction during primary total knee arthroplasty(TKA). Methods A total of 14 cases(14 knees)with proximal tibial bone defect who underwent primary TKA during Jan. 2009 and Jan. 2014 were enrolled, including 3 males and 11 females. The average age of patients was 59.3 years, ranging from 51 to 80 years. The diagnoses were severe osteoarthritis(6 cases of varus deformity and 1 case of valgus deformity), rheumatoid arthritis(5 cases), and post-traumatic osteoarthritis(2 cases). Bone cement was used in 5 cases, and prosthetic augments was adopted in 9 cases. Results All patients were followed up for an average of 26 months, ranging from 12 to 48 months. The postoperative mechanical alignment and competent position were satisfactory. The knee function scores rose from 46.23 before operation to 82.5 on average. No severe complications occurred. Conclusion The reconstruction technique including bone cement and modular augment is effective in the treatment of proximal tibia bone defect.

Analgesic effect of continuous thoracic paravertebral block in patients with multiple fractured ribs

ZHANG Zhenwei, FAN Xiaoyan, ZHANG Yunhua, JIANG Zhongmin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  39-42.  doi:10.6040/j.issn.1671-7554.0.2016.313

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Objective To observe the analgesic effect of continuous thoracic paravertebral block in patients with multiple fractured ribs. Methods A total of 92 patients with unilateral fractured ribs were randomly divided into 2 groups: TPVB group(n=46, received continuous ropivacaine infusion through thoracic paravertebral block)and MOP group(n=46, received various dosages of morphine). Visual analog scale scores(VAS)at rest and on coughing, and respiratory rate were measured before and after administration of the block. The postoperative 12-hour and 24-hour oxygen partial pressure(PO₂)and partial pressure of carbon dioxide(PCO₂), and 5-day post-injury pleural effusion quantity were detected. Results After administration of block, the mean VAS and respiratory rate decreased significantly(P<0.05). The average PO₂ in the TPVB group were higher than that in the MOP group(P<0.05), while the average PCO₂ were significantly lower in the TPVB group than that in the MOP group(P<0.05). The 5-day post-injury pleural effusion quantity of TPVB group was less than that of MOP group(P<0.05). Conclusion Continuous thoracic paravertebral block significantly improves pulmonary function and relieves pain, and shortens pulmonary atelectasis in patients with multiple fractured ribs.

Do we really need wound drainage in hip arthroplasty? A meta-analysis

XU Chuang, QI Dachun, LI Xianrang, ZU Meng, LI Jian, LIU Mingting

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  43-49.  doi:10.6040/j.issn.1671-7554.0.2015.757

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Objective To explore the efficacy and safety of wound drainage in hip arthroplasty by meta-analysis. Methods The criteria for inclusion and exclusion were formulated. The following databases were searched, including PubMed, EBSCO, Cochrane Library, WanFang Database and China National Knowledge Infrastructure(CNKI)for studies published during Jan. 2000 and Aug. 2015, and then the randomized trials on hip arthroplasty with wound drainage system and without drainage system were compared. Meta-analysis were conducted with Review Manager 5.2. Results A total of 20 studies involving 2 270 participants(1 134 drains and 1 136 without drains)were included in our meta-analysis. There was no significant difference in the incidence of wound infections (RR=0.75, 95%CI: 0.49-1.14, P=0.18), wound hematomas(RR=0.98, 95%CI: 0.25-3.91, P=0.98), deep vein thrombosis (RR=2.56, 95%CI: 0.86-7.68, P=0.09), pulmonary embolism(RR=2.37, 95%CI: 0.35-15.94, P=0.38)or pain between the two groups. Our results demonstrated blood transfusion(RR=1.36, 95%CI: 1.18-1.58, P<0.001), transfusion unit(WMD=0.64, 95%CI: 0.21-1.07, P<0.05)and blood loss(WMD=277.80, 95%CI: 118.88-436.73, P<0.05)were more frequent in those who received drains. Conclusion The routine use of wound drainage for hip arthroplasty has no obvious advantages. However, there is a moderate possibility of selection bias and publication bias in this review. More samples and high-quality randomized trials are needed to increase the reliability of evidences.

Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma

WEI Xiujuan, WU Xiuyin, TONG Dongdong, LI Jing, YANG Xiaolu, ZHANG Fenghe

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  50-54.  doi:10.6040/j.issn.1671-7554.0.2015.1222

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Objective To investigate the expressions of brain-derived neurotrophic factor(BDNF)and its primary receptor tyrosine kinase receptor B(TrkB)in tongue squamous cell carcinoma(TSCC), and to explore the effect of BDNF on the proliferation of TSCC. Methods The expressions of BDNF and TrkB in 50 TSCC and 13 normal mucosa tissues were detected with immunohistochemical method. The expressions of BDNF and TrkB in human tongue squamous cell carcinoma cell lines(Tca8113)were examined with immunofluorescence technique. Cell viability of Tca8113 was detected with CCK8 assay. Results The positive rate of staining of BDNF and TrkB in TSCC was significantly higher than that in normal tongue mucosa(P<0.05). In addition, the rate of positive expression was related to infiltration depth and lymph node metastasis. BDNF(200 ng/mL)significantly promoted the proliferation of Tca8113. Conclusion BDNF and TrkB may be closely associated with the genesis and development of TSCC. Moreover, BDNF may promote cell proliferation of TSCC.

An improved connective tissue flap technique applied in alveolar ridge crest for aesthetic implant restoration in maxillary anterior zone

SUN Ruinan, ZHU Zhenkun, JIA Zhao, ZHOU Qin, XU Xin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  55-60.  doi:10.6040/j.issn.1671-7554.0.2015.1352

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Objective To evaluate the effect of an improved connective tissue flap technique applied in alveolar ridge crest for aesthetic implant restoration in maxillary anterior zone. Methods A total of 20 patients with flat or concave profile of labial soft tissue on implant site before second-stage surgery who received the improved connective tissue flap technique during second-stage surgery were enrolled as the experimental group; 20 patients according with the inclusive criteria who received second-stage surgery without the improved connective tissue flap technique were involved as the control group. We observed and measured the convex profile on the facial aspect, relative height of gingival papilla and pink esthetic score(PES)at the follow time points: before surgery; 2 weeks, 1 month, 6 months and 12 months after the second-stage surgery. The Data were analyzed with t-test and repeated measure. Results The convex profile was improved after second-stage surgery in the experimental group(T1-T0: 0.3; T2-T0: 0.27; T3-T0: 0.26; T4-T0: 0.26). There were significant difference between the two groups(P<0.001). Both long-term and short-term effects were satisfactory. There was no significant difference in the relative height of gingival papilla and PES between the two groups(P>0.05). Conclusion The improved connective tissue flap technique can be considered an effective method 山 东 大 学 学 报 (医 学 版)54卷6期 -孙睿男,等.改良牙槽嵴顶结缔组织转瓣技术在上颌前牙种植美学修复中的应用 \=-to augment the soft tissue during second-stage surgery without causing the shrinkage of gingival papilla or decrease of PES. It is worth wide application in soft tissue aesthetics of implant therapy.

Clinical predictors of remote lesions for patients with acute ischemic stroke

LI Fan, SHANG Wei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  61-64.  doi:10.6040/j.issn.1671-7554.0.2016.010

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Objective To explore the clinical and image factors associated with remote lesions in patients with acute ischemic stroke. Methods Clinical data of 106 patients with acute middle cerebral artery occlusion treated during June and Dec., 2014 were retrospectively reviewed. The patients were divided into the positive group and negative group according to imaging findings. The baseline characteristics were compared with univariate analysis. The outcome after 3 months were followed up and evaluated with mRS score. Results Of all 106 patients, 39 patients(36%)had positive imaging findings. The 3-month outcome was worse than the negative group(mRS score, U=1 446.500, P=0.022). Univariate analysis showed that positive group had higher admission NIHSS score than the negative group(U=960.000, P<0.01), and the positive group had lower rate of improvement than the negative group(24-hour NIHSS score decreased≥4 points, or down to 0; χ2=19.758, P<0.01). Conclusion Admission NIHSS score and early symptom improvement were the factors associated with remote lesions.

Effects of parecoxibsodium on inflammatory response and its neuroprotection in patients undergoing brain tumor surgery

YU Yujuan, GAO Chengjie, WANG Xiaopeng, LI Bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  65-68.  doi:10.6040/j.issn.1671-7554.0.2015.998

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Objective To observe the effects of parecoxibsodium on serum levels of tumor necrosis factor-α(TNF-α), interleukin 1β(IL-1β), neuron-specific enolase(NSE)and S100β protein in patients undergoing brain tumor surgery, and to investigate its neuroprotection effects. Methods Forty patients undergoing brain tumor surgery under general anesthesia were randomly divided into the parecoxibsodium group and the control group, twenty in each group. The parecoxibsodium group was injected intravenously 40 mg parecoxibsodium(dissolved in 5 mL saline solution)before and 12 hours after the anesthesia induction, the control group received 5 mL saline solution in the same way. The serum levels of TNF-α, IL-1β, NSE and S100β protein were measured before anesthesia induction(T₀), after removing the tumor(T₁), the completion of surgery(T₂), 6 h after surgery(T₃)and 24 h after surgery(T₄). Results Compared with the control group, the levels of TNF-α, IL-1β, NSE and S100β protein in the parecoxibsodium group obviously decreased at the time points of T₁-T₄(P<0.01). Compared with the time point of T₀, the levels of TNF-α, IL-1β, NSE and S100β protein in two groups obviously increased at the time points of T₁-T₄(P<0.01). Conclusion Parecoxibsodium can inhibit the expressions of TNF-α, IL-1β and decrease the levels of NSE and S100β protein in the serum, and it can provide neuroprotection effects through alleviating the inflammation response during brain tumor surgery.

Influencing factors on the curative effect of microvascular decompression in the treatment of primary trigeminal neuralgia

ZHANG Bin, ZHANG Liangwen, CHI Lingyi, WANG Chuanwei, ZHU Shugan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  69-72.  doi:10.6040/j.issn.1671-7554.0.2015.1099

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Objective To explore the influencing factors on the curative effect of microvascular decompression(MVD)in the treatment of primary trigeminal neuralgia(TN). Methods MVD was performed in 153 primary TN patients. The independent risk factors on the curative effect were evaluated by single factor analysis of chi-square test and Logistic regression analysis. Results (1) Pain vanished in 125 cases, obviously relieved in 26 cases, and remained unchanged in 2 cases. (2) The artery was compressed in 103 cases, complicated with venous conflict in 49 cases, and 1 case had no obvious conflict. The pain-free rate of venous conflict(71%)was significantly lower than that of artery compression alone(81%, P<0.05). (3) The pain-free rate of V2 was significantly lower than others(P<0.05). Half of the patients with V2 had their NVC at the ventral site of the nerve(P<0.05). (4) Logistic regression analysis showed the vein and V2 were independent risk factors on the curative effect. Conclusion Patients with venous compression or V2 pain have poor curative effect. Venous compression and V2 pain are the independent risk factors. The ventral site is the crucial conflict site of V2, which can serve as a new target for the study of refractory trigeminal neuralgia.

Clinicopathologic features of Hashimotos thyroiditis complicated with papillary thyroid cancer

HAO Zhanyu, LI Bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  73-77.  doi:10.6040/j.issn.1671-7554.0.2016.333

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Objective To analyze the clinicopathologic features of Hashimotos thyroiditis(HT)complicated with papillary thyroid cancer(PTC). Methods A total of 297 patients with PTC treated surgically during Oct. 2013 and Oct. 2015 were divided into 2 groups: experimental group(PTC complicated with HT, n=49, 5 males and 44 females, aged 24-67 years, 27 cases < 45 years), and control group(PTC only, n=248, 60 males and 188 females, aged 17-76 years, 97 cases < 45 years). The clinical data of the 2 groups were compared and analyzed statistically, including gender, age, diffusely enlarged thyroid gland, level of serum autoimmune antibody, capsular invasion, tumor size, intraoperative frozen pathology, and TNM staging. Results There were statistically significant differences between the 2 groups in gender, age, diffusely enlarged thyroid gland, level of serum autoimmune antibody and central lymph node metastasis(all P<0.05), but no statistical differences in capsular invasion, tumor size, intraoperative frozen pathology and TNM staging(all P>0.05). Conclusion HT complicated with PTC is more common in female patients, especially young women. It usually manifests as diffusely enlarged thyroid, increased serum autoimmune antibody and early lymph node metastasis. HT patients should check regularly for timely diagnosis and treatment.

Diagnostic value of color Doppler ultrasound for patients with anterior tibial artery steal due to atherosclerotic occlusive disease in lower extremity

WU Shixiu, LI Jie, JIA Huigang, LI Jine, CUI Linling, LU Hong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  78-81.  doi:10.6040/j.issn.1671-7554.0.2015.853

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Objective To explore the diagnostic value of color Doppler ultrasound for patients with anterior tibial artery steal due to atherosclerotic occlusive disease in the lower extremity. Methods A total of 30 patients were enrolled as the case group, while 30 healthy volunteers served as healthy controls(the control group). All patients received color Doppler ultrasound examination of the anterior tibial artery(ATA), medial anterior malleolarartery(MAM), lateral malleolus anteriorartery(LAM), medial posterior malleolarartery(MPM), and lateral posterior malleolarartery(LPM). The arterial diameter(D), blood flow direction and peak systolic velocity(PSV)were recorded. Results In the case group, there were 48 anterior tibial artery steal, 43 countercurrent MAM and LAM, 3 countercurrent MAM, 2 countercurrent LAM. Compared with the control group, MAM, LAM and MPM, LPM lumen widened, flow velocity increased, and the difference was statistically significant(P<0.05, P<0.01). Conclusion Color Doppler ultrasound can sensitively identify anterior tibial artery steal and permit quantification of the medial malleolus and lateral artery net. Therefore, it is significant in the clinical diagnosis of anterior tibial artery steal.

Screening results and influencing factors of T-SPOT.TB assay in patients with autoimmune rheumatic diseases

GAI Linlin, GUAN Lixue, LI Haibo, CHU Jinjin, ZHU Yun, LI Ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  82-86.  doi:10.6040/j.issn.1671-7554.0.2015.713

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Objective To investigate the status of latent tuberculosis infection(LTBI)in patients with autoimmune rheumatic diseases and the factors associated with a T-cell enzyme-linked immunospot assay(T-SPOT.TB). Methods During June 2013 and June 2014, a total of 223 patients with various autoimmune rheumatic diseases(patient group)and 53 healthy subjects(control group)were enrolled and screened for LTBI by T-SPOT.TB tests. Data on tuberculosis exposure, use of glucosteroid and disease-modifying anti-rheumatic drugs(DMARDs), BCG vaccination history and abnormal CT scans were collected. Miettinen test was used to estimate odds ratios(OR)and 95% confidence intervals(CI). Results T-SPOT.TB-positive rate was 20.18% in the patient group and 5.66% in the control group, and the patient group had higher risk of LTBI(OR=4.21, 95%CI: 1.21-14.70, P<0.05). There were significant 山 东 大 学 学 报 (医 学 版)54卷6期 -盖林林,等.免疫性疾病患者T-SPOT.TB筛查结果及影响因素 \=-differences in T-SPOT.TB-positive rate and OR among various autoimmune rheumatic diseases. The T-SPOT.TB positive rate was 44.0% in patients with erythema nodosumts(OR=13.11, 95%CI: 3.44-49.98, P<0.01), and 21.16% in patients with rheumatoid arthritis(RA)(OR=4.47, 95%CI: 1.06-18.82, P<0.05), obviously higher than that of controls. The T-SPOT.TB positive rate in patients with other rheumatic autoimmune diseases was 10.0%-33.34%(OR=1.85-8.3, P>0.05). There were no significant differences between the proportions of patients treated with glucocorticoid and DMARDs in T-SPOT.TB-positive and T-SPOT.TB-negative groups(P>0.05). Previous active TB and an abnormal CT examination were significantly associated with T-SPOT.TB-positive rate(P<0.01). Conclusion Patients with autoimmune rheumatic diseases are at increased risk of LTBI(OR=4.21). The glucocorticoid and DMARDs treatment have little effect on T-SPOT.TB results. It is an effective method to screen LTBI with T-SPOT.TB in patients with autoimmune rheumatic diseases.

Correlation of QuantiFERON-TB gold in-tube test result with age of adults

BU Fanfeng, SUN Xiaxia, LIANG Xiaoling

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  87-90.  doi:10.6040/j.issn.1671-7554.0.2015.493

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Objective To explore the relationship between age and release of interferon gamma in blood of adults through the correlation analysis of age and QuantiFERON-TB gold in-tube test(QFT-GIT)results. Methods A total of 168 patients with positive results of QFT-GIT were divided into three groups according to the age:18 to 40 years group, 41 to 65 years group and elder than 65 years group. The changes of IFN-γ response to TB-specific antigen(TB Ag-Nil)and non-specific phytohemagglutinin(Mitogen-Nil)were compared among the three groups. Spearman correlation analysis was used to calculate the correlation coefficient value γ. Linear regression analysis was used to show the change. Results The change trend of TB Ag-Nil and Mitogen-Nil in 18 to 40 years group and 41 to 65 years group were not statistically significant(P>0.05). However in patients elder than 65 years, the levels of TB Ag-Nil and Mitogen-Nil decreased significantly with age increasing(P<0.05). Conclusion For patients of 18 to 65 years, the results of QFT-GIT are not related to the age, but for those elder than 65 years, TB Ag-Nil and Mitogen-Nil is negatively related to the age.

Health inequality related to income and influencing factors of the rural elderly in three counties of Shandong Province

LI Yan, LIU Kun, SUN Xiaojie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(6):  91-96.  doi:10.6040/j.issn.1671-7554.0.2015.990

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Objective To investigate the income-related health inequality among the rural elderly in Shandong Province, in order to provide information for policy making. Methods Survey data of 975 rural elderly were collected in Shandong Province in 2013 to analyze the health inequality and influencing factors. Results The inequality existed to a larger extent among the poor, which was closely related to income, followed by social capital. In the 4 dimensions of social capital, domestic resource was the most important influencing factor for inequality in terms of physical and mental health. Conclusion Income and social capital contributed most to the health inequality. Efforts should be made to increase family income and social capital among the rural elderly.