JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 12-15.doi: 10.6040/j.issn.1671-7554.0.2015.855

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Down-regulation of α-catulin inhibits the invasion and migration of human tongue squamous cancer cell line Tscca in vitro

MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan   

  1. Department of Stomatology, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2015-09-10 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To explore the effect of α-catulin down-regulation on the invasion and migration of tongue squamous cancer cell line Tscca. Methods Cells were divided into α-catulin-siRNA transfection group, empty plasmid transfection group, and blank group. The α-catulin down-expressing plasmid was constructed and transfected into human tongue squamous cancer cell line Tscca. The protein expression of α-catulin was tested by Western blotting. The in vitro invasion and migration abilities of Tscca cells were measured by Transwell assays and Wound Healing. Results The protein expression of α-catulin reduced remarkably in α-catulin-siRNA trasfection group. Wound-healing and Transwell assays showed that the invasion and migration of Tscca cells were suppressed in α-catulin-siRNA transfection group. Conclusion SiRNA-mediated silencing of the α-catulin gene can significantly inhibit cell invasion and migration in human squamous cell carcinoma line Tscca, indicating that α-catulin might be used as an underlying target for molecular therapy of tongue cancer.

Key words: α-catulin, Migration, Tongue squamous cell carcinoma, Invasion

CLC Number: 

  • R739.86
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