JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 11-17.doi: 10.6040/j.issn.1671-7554.0.2016.132

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Role of human TIMP-2 protein in the migration and invasion of hepatocellular carcinoma cell

WANG Wei1, CAO Yushan1, SUN Daquan2, HUANG Xiaoqiong1, XU Guoqiang1   

  1. 1. Department of Physiology;
    2. Department of Biochemistry &
    Molecular Biology, Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2016-02-08 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To collect protein of human TIMP-2 through prokaryotic expression in vitro, and to explore its role in the migration and invasion of hepatocellular carcinoma(HCC)cell. Methods Total RNA was extracted from human hepatoma cell line SMMC-7721, and TIMP-2 cDNA was obtained with RT-PCR. The prokaryotic expression vector pGEX-TIMP-2 was constructed, and then transformed into Origami(DE3). The expressional product was purified with affinity chromatography and identified with Western blotting. The role of TIMP-2 in migration and invasion of HCC cells were detected with RNAi technology and adding extraneous TIMP-2 protein. The mRNA and protein expressions of endogenous TIMP-2 in HCC cells were detected with real-time fluorescence quantitative PCR and Western blotting after transfection with siTIMP-2. Results Adding of exogenous TIMP-2 suppressed the migration and invasion of HCC; knock-down of TIMP-2 enhanced the migration and invasion of HCC; conditioned medium of hepatic stellate cells promoted the migration and invasion of HCC, which could be inhibited by TIMP-2 protein. Conclusion The level of TIMP-2 protein in the microenvironment is closely related to the migration and invasion of HCC.

Key words: Tissue inhibitor of matrix metalloproteinases-2, Invasion, Hepatocellular carcinoma, Migration

CLC Number: 

  • R735.7
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