JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (3): 14-18.doi: 10.6040/j.issn.1671-7554.0.2015.833

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Effect of tumor associated macrophages on the expression of c-Met in hepatocellular carcinoma cells

ZONG Zhaoyun1, LI Xia2, HAN Zhenlong3, WANG Xianteng1, GUO Chun1, ZHANG Lining1, SHI Yongyu1   

  1. 1. Department of Immunology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Oncology, Jinan First Peoples Hospital, Jinan 250011, Shandong, China;
    3. Department of Clinical Laboratory, General Hospital of Police Officer, Jinan 250002, Shandong, China
  • Received:2015-09-01 Online:2016-03-10 Published:2016-03-10

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Abstract: Objective To explore the molecular mechanism of c-Met expression in hepatocellular carcinoma cells(HCC)influenced by tumor associated macrophages. Methods HCC cells were cocultured with macrophages derived from THP-1 cells. The expression of c-Met in HCC cells was detected at mRNA level by RT-PCR and protein level by flow cytometry. The migration of HCC cells was detected by Transwell cell migration test. Results The expression of c-Met in HCC cells was upregulated by the macrophages at both mRNA and protein levels. After HCC cells were cocultured with macrophages, the migration was enhanced in the presence of human recombinant protein hepatocyte growth factor(HGF). Conclusion Tumor associated macrophages promote the migration of HCC cells by upregulating the expression of c-Met in HCC cells.

Key words: Tumor associated macrophages, Cellular-mesenchymal to epithelial transition factor, Hepatocellular carcinoma cells, Hepatocyte growth factor

CLC Number: 

  • R574
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