JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 18-22.doi: 10.6040/j.issn.1671-7554.0.2015.610

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Pulsatilla saponin D combined with sorafenib against hepatocellular carcinoma cell invasion and metastasis

HE Wubin1, SU Rongjian2   

  1. 1. Biological Therapy Central Laboratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, Liaoning, China;
    2. Life Science Institute of Jinzhou Medical University, Jinzhou 121001, Liaoning, China
  • Received:2015-06-24 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To investigate the effect of Pulsatilla saponin D and sorafenib on the invasion and metastasis of human hepatoma cell line. Methods The human hepatoma cell line SMMC-7721 cells were divided into Pulsatilla saponin D group(11.9 μg/L), sorafenib group(2.15 μmol/L), combined group(Pulsatilla saponin D 11.9 μg/L+Sorafenib 2.15 μmol/L)and control group(ordinary culture). The inhibitory effects on the migration and invasion of SMMC-7721 cells were observed with MTT assay and Transwell chamber experiments. The effects on CD44V6, VEGF-C, MMP-9 gene protein expressions were detected with immunocytochemistry. Results MTT assay showed that Pulsatilla saponin D, sorafenib, and combined therapy inhibited the proliferation of SMMC-7721 cells, and the 24 h inhibition rate was less than 15%. Transwell chamber experiments showed that the adhesion inhibition rate of the combined group displayed a synergistic effect compared with the monotherapy groups, but not in a time-dependent manner; the invasion and migration inhibition rate of the combined group were significantly higher than those of the monotherapy groups(P<0.01). Immunohistochemistry results showed that the combined group had lower CD44V6, VEGF-C, and MMP-9 expressions than the monotherapy groups(P<0.05). Conclusion Pulsatilla saponin D and sorafenib synergistically inhibit the SMMC-7721 cell invasion and metastasis, and the combined effect of Pulsatilla saponin D and sorafenib is 山 东 大 学 学 报 (医 学 版)54卷7期 -贺武斌,等.白头翁皂苷D联合索拉非尼对人肝癌细胞侵袭与转移的影响 \=-better than the single drug.

Key words: Pulsatilla saponin D, Sorafenib, Invasion, Hepatoma cells, Metastasis

CLC Number: 

  • R735.7
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