JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.666

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Galectin-9 inhibits proliferation and migration of glioma cells

YAO Zhong1, CHEN Weiliang1, XU Yangyang1, HE Ying2, QU Xun2, LI Xingang1   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, Brain Science Research Institute, Shandong University, Jinan 250012, Shandong, China;
    2. Institute of Basic Medical Sciences, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-10-08 Revised:2014-12-23 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To investigate the effects of exogenous galectin-9 on the proliferation and migration of glioma cells and its potential mechanism. Methods The expression of galectin-9 in glioma cells was detected with real-time quantitative polymerase chain reaction (PCR). After glioma cell line U251 was treated with different concentrations of exogenous galectin-9 (1, 4, 8, 16 μg/mL), the changes of proliferation and migration were tested with cell count kit-8 (CCK-8) and transwell chambers respectively at different time (12, 24, 36 h). When the competitive inhibitor lactose was added, the changes in proliferation and migration were detected. Results The expression of galectin-9 in 4 glioma cell lines was low or barely detected. Cells treated with 8 and 16 μg/mL galectin-9 showed decreased cell proliferation rates, compared with control group (P <0.001). With time passing by, the cell proliferation rate decreased gradually (P <0.01). After lactose treatment, cell proliferation rate increased (P <0.05). Galectin-9 treatment significantly reduced the numbers of cells penetrating the micropomus membrane (P <0.01), while lactose treatment increased the number of cells, compared with the untreated group (P<0.05). Conclusion Galectin-9 effectively inhibits proliferation of glioma cells in a time- and dose-dependent manner, and it can also inhibit the migration of glioma cells.

Key words: Proliferation, Migration, Glioma, Galectin-9

CLC Number: 

  • R739.4
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