JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (11): 1-6.doi: 10.6040/j.issn.1671-7554.0.2016.1425

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Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells

SUN Zeyu1, CHEN Ying1, LIN Jiaxiang2, LIU Juan1, ZHAO Weiming1   

  1. 1. Department of Pathogenic Biology;
    2. Department of Pathology, School of Basic Medical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-11-03 Online:2017-11-10 Published:2017-11-10

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Abstract: Objective To study the effect of inositol polyphosphate-4-phosphatase type II(INPP4B)on the proliferation,cell cycle and apoptosis of cervical cancer cells. Methods Western blotting was employed to detect basic expression of INPP4B in various kinds of cervical cancer cell lines. The INPP4B expressions in LHCX-INPP4B transfected SiHa and C33a cells as well as INPP4B specific siRNA transfected CaSki cells were determined by Western blotting. CCK-8 and colony formation assay were used to test cell growth and proliferation. Cell cycle was detected by using BrdU assay. Cell apoptosis was evaluated using Annexin V-FITC/PI cell apoptosis kit. Results CCK-8 and clone formation assay showed that the overexpression of INPP4B significantly reduced the cell viability and clonogenic ability of SiHa and C33a(all P<0.05). BrdU assay showed that the proportion of SiHa and C33a cells in S phase decreased after being transfected with INPP4B(P=0.04, P=0.01). Accordantly, depletion of INPP4B in Caski cells increased cell viability and the percentage of the cells entering S phase(P<0.01). However, INPP4B did not influence the apoptosis of cervical cancer cells. Conclusion INPP4B inhibits the growth and proliferation of cervical cancer cells and leads to cell cycle arrest.

Key words: Inositol polyphosphate-4-phosphatase type II, Cycle, Cervical cancer, Proliferation

CLC Number: 

  • R737
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