Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (9): 53-58.doi: 10.6040/j.issn.1671-7554.0.2021.1471

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Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells

ZHAO Ge1, ZOU Cunhua1, SONG Dongdong2, ZHAO Shuping1   

  1. 1. Gynecological Center;
    2. General Surgery Qingdao Women and Children Hospital, Qingdao 266034, Shandong, China
  • Published:2022-09-02

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Abstract: Objective To investigate the effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells. Methods According to the experimental requirements, endometrial carcinoma cells treated with tanshinone IIA were divided into the control group, low concentration group(1 μg/mL), medium concentration group(2.5 μg/mL)and high concentration group(5 μg/mL). Meanwhile, cells co-treated with CK2 specific phosphorylation inhibitor(TBB)and tanshinone IIA were divided into the control group, TBB group(60 μmol), drug group(5 μg/mL)and drug(5 μg/mL)+ TBB group(60 μmol). The effects of tanshinone IIA on the proliferation of cells were detected with crystal violet staining; expressions of P53, Cleaved caspase-3, B-cell lymphoma-2(Bcl-2), casein kinase 2α(CK2α), deletion in breast cancer 1(DBC1), silent information regulator 1(SIRT1)and p-deletion in breast cancer 1(p-DBC1)were detected with Western blotting. After TBB was added, expressions of CK2α, DBC1 and p-DBC1 were also detected. Results The results of crystal violet staining showed that the proliferation rates of the control group and high concentration group at each time point(24 h, 48 h, 72 h)were(0.87±0.05)%,(0.61±0.04)%,(0.30±0.04)% and(0.09±0.04)%, respectively(F=217.976, P<0.001). The results of Western blotting showed that under the action of tanshinone IIA, the expressions of P53(F=244.261)and Cleaved caspase-3(F=290.842)increased in a concentration-dependent manner, while the expression of Bcl-2(F=198.170)decreased in a concentration-dependent manner(P<0.001), and the expression of p-DBC1/DBC1 decreased in a concentration-dependent manner(F=187.092, P<0.001), but there was no significant difference in CK2α and SIRT1 expressions(P>0.05). The expression of p-DBC1/DBC1 was inhibited by TBB(F=7.847), tanshinone IIA(F=827.715)and their combination(F=22.174), and the difference was statistically significant compared with the control group(P=0.023,P<0.001, P=0.002), but there was no significant difference in CK2α expression(P>0.05). Conclusion Tanshinone IIA can significantly inhibit the proliferation and induce apoptosis of endometrial carcinoma cells, and the mechanism may be related to the CK2/DBC1/SIRT1/P53 signaling pathway.

Key words: Tanshinone IIA, Endometrial carcinoma, Apoptosis, Cell proliferation, Phosphorylation

CLC Number: 

  • R737.33
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