Journal of Shandong University (Health Sciences) ›› 2020, Vol. 1 ›› Issue (7): 15-23.doi: 10.6040/j.issn.1671-7554.0.2019.1273

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Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice

LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde   

  1. Department of Human Anatomy, School of Basic Medical Science, Guizhou Medical University, Guian 550025, Guizhou, China
  • Online:2020-07-20 Published:2020-07-10

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Abstract: Objective To investigate the effect of injection of Noggin protein into the lateral ventricle on the learning and memory abilities and the structure of dentate gyrus after cerebral ischemia reperfusion injury(CIRI)in mice, in order to provide new ideas for the prevention and treatment of clinical ischemic cerebrovascular diseases. Methods A total of 240 health mice were randomly divided into the sham group(n=80), the ischemia-reperfusion group(I/R group, n=80), and the Noggin treatment group(Noggin group, n=80). Each group was subdivided into the 1days, 3 days, 7 days and 14 days subgroups. The learning and memory abilities were detected by Y-maze at 1 day before execution. Infarct size was detected by triphenyltetrazolium chloride staining(n=5). The colorimetric method was used to detect the superoxide dismutase(SOD)and malondialdehyde(MDA)activity after the behavior capability determined(n=5). Infarct size was detected by 2,3,5-triphenyl tetrazolium chloride staining(n=5). Glial fibrillary acidic protein(GFAP)immunopositive cells in the DG were observed under a light microscopy and quantitative analysis were performed by cell morphometric technique(n=5). The BMP4 protein expression was detected by Western blotting(n=5). Results Compared with the sham group, the neurological function score of mice in the I/R group and Noggin group increased(Fgroup=21.19, P<0.001; Ftime=25.13, P<0.001), learning and memory abilities declined(Fgroup=216.10, P<0.001; Ftime=260.10, P<0.001)(Fgroup=114.40, P<0.001; Ftime=184.60, P<0.001), and area of cerebral infarction increased(Fgroup=2 374, P<0.001; Ftime=3 292, P<0.001), SOD activity reduced(Fgroup=1 426, P<0.001; Ftime=1 723, P<0.001)and MDA content increased(Fgroup=2.22, P<0.001; Ftime=6.33, P<0.001), number of neurons reduced(Fgroup=148.90, P<0.001; Ftime=485.50, P<0.001), and expression of GFAP positive cells and BMP4 protein increased(Fgroup=40.18, P<0.001; Ftime=141.90, P<0.001)(Fgroup=426.70, P<0.001; Ftime=1 329, P<0.001)with the increase of injury time. At each same time point, compared with the I/R group, the Noggin groups neurological function score decreased(P<0.001), learning and memory abilities increased(P<0.001), and cerebral infarction area decreased(P<0.001), SOD activity increased and MDA content decreased(P<0.001), number of neurons increased and staining was lightened (P<0.001), expression of GFAP positive cells and BMP4 protein decreased(P<0.001). Conclusion Noggin can efficiently improve the learning and memory abilities and decrease the pathological lesion of DG after cerebral ischemia reperfusion injury in mice, and its mechanism may closely related to BMP4 protein expression and glial cells activation.

Key words: Noggin protein, Cerebral ischemia reperfusion injury, Dentate gyrus, Bone morphogenetic protein 4, Glial fibrillary acidic protein, Mouse, zw

CLC Number: 

  • R743.31
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