Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (6): 47-52.doi: 10.6040/j.issn.1671-7554.0.2019.1452

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Expression and role of toll-like receptor 4 in adenomyosis

WANG Bingyu, YANG Yang, CHAO Lan   

  1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explored the role of toll-like receptor 4(TLR4)in the pathogenesis of adenomyosis(AS)and the relationship with apoptosis. Methods The protein and mRNA expressions and distribution of TLR4 in the endometrium of AS patients and normal controls were detected with Western blotting, real-time PCR and immunohistochemistry, respectively. The cell apoptosis was determined with TUNEL. After the expression of LTR4 in h-ESC cell line was activated with lipopolysaccharide(LPS), the level of apoptosis was detected with flow cytometry in order to evaluate the effect of TLR4 on apoptosis. Results The protein expression( P=0.0128)and mRNA expression(P=0.0197) of TLR4 were lower in AS endometrium than in normal endometrium. TLR4 mainly distributed in the glandular epithelial cytoplasm in endometrium, and TLR4 expression was lower in AS endometrium than in normal endometrium (P=0.0160). The apoptosis level was lower in AS endometrium than in normal endometrium (P=0.0024). After TLR4 was activated in h-ESC cell line, the number of cells in the lower right quadrant increased, indicating elevated apoptosis. Conclusion TLR4 plays an important role in adenomyosis by regulating apoptosis.

Key words: Adenomyosis, Endometrium, TLR4, Apoptosis

CLC Number: 

  • R711.71
[1] Pontis A, DAlterio MN, Pirarba S, et al. Adenomyosis: a systematic review of medical treatment [J]. Gynecol Endocrinol, 2016, 32(9): 696-700.
[2] Nikkanen V, Punnonen R. Clinical significance of adenomyosis [J]. Ann Chir Gynaecol, 1980, 69(6): 278-280.
[3] Ota H, Igarashi S, Hatazawa J, et al. Is adenomyosis an immune disease [J] Hum Reprod Update, 1998, 4(4): 360-367.
[4] Munro MG. Uterine polyps, adenomyosis, leiomyomas, and endometrial receptivity [J]. Fertil Steril, 2019, 111(4): 629-640.
[5] Liu Y, Tan X, Wang Z, et al. Down-regulation of tumor suppressor PDCD4 expression in endometrium of adenomyosis patients [J]. Curr Res Transl Med, 2016, 64(3): 123-128.
[6] Buggio L, Monti E, Gattei U, et al. Adenomyosis: fertility and obstetric outcome. A comprehensive literature review[J]. Minerva Ginecol, 2018, 70(3): 295-302.
[7] Orazov MR, Nosenko EN, Radzinsky VE, et al. Proangiogenic features in chronic pelvic pain caused by adenomyosis [J]. Gynecol Endocrinol, 2016, 32(sup2): 7-10.
[8] Vannuccini S, Tosti C, Carmona F, et al. Pathogenesis of adenomyosis: an update on molecular mechanisms [J]. Reprod Biomed Online, 2017, 35(5): 592-601.
[9] Kvaskoff M, Mu F, Terry KL, et al. Endometriosis: a high-risk population for major chronic diseases? [J]. Hum Reprod Update, 2015, 21(4): 500-516.
[10] Mu F, Rich-Edwards J, Rimm EB, et al. Endometriosis and risk of coronary heart disease [J]. Circ Cardiovasc Qual Outcomes, 2016, 9(3): 257-264.
[11] Kishi Y, Shimada K, Fujii T, et al. Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium[J]. PLoS One, 2017, 12(12): e0189522. doi:10.1371/journal.pone.0189522.
[12] Aznaurova YB, Zhumataev MB, Roberts TK, et al. Molecular aspects of development and regulation of endometriosis [J]. Reprod Biol Endocrinol, 2014, 12: 50. doi:10.1186/1477-7827-12-50.
[13] Elmore S. Apoptosis: a review of programmed cell death [J]. Toxicol Pathol, 2007, 35(4): 495-516.
[14] Pistritto G, Trisciuoglio D, Ceci C, et al. Apoptosis as anticancer mechanism: function and dysfunction of its modulators and targeted therapeutic strategies [J]. Aging(Albany NY), 2016, 8(4): 603-619.
[15] Agui T, McConkey DJ, Tanigawa N. Comparative study of various biological parameters, including expression of survivin, between primary and metastatic human colonic adenocarcinomas [J]. Anticancer Res, 2002, 22(3): 1769-1776.
[16] Khan KN, Masuzaki H, Fujishita A, et al. Immunoexpression of hepatocyte growth factor and c-Met receptor in the eutopic endometrium predicts the activity of ectopic endometrium [J]. Fertil Steril, 2003, 79(1): 173-181.
[17] Harada M., Suganuma N, Furuhashi M, et al. Detection of apoptosis in human endometriotic tissues [J]. Mol Hum Reprod, 1996, 2(5): 307.
[18] Afkham A, Eghbal-Fard S, Heydarlou H, et al. Toll-like receptors signaling network in pre-eclampsia: An updated review [J]. J Cell Physiol, 2019, 234(3): 2229-2240.
[19] Azam S, Jakaria M, Kim LS, et al. Regulation of toll-like receptor(TLR)signaling pathway by polyphenols in the treatment of age-linked neurodegenerative diseases: focus on TLR4 signaling [J]. Front Immunol, 2019, 10: 1000. doi:10.3389/fimmu.2019.01000.
[20] Karaffov a V, Marcinkov a E, Bobikov a K, et al. TLR4 and TLR21 expression, MIF, IFN-beta, MD-2, CD14 activation, and sIgA production in chickens administered with EFAL41 strain challenged with campylobacter jejuni[J]. Folia Microbiol(Praha), 2017, 62(2): 89-97.
[21] Hu L, Yang H, Ai M, et al. Inhibition of TLR4 alleviates the inflammation and apoptosis of retinal ganglion cells in high glucose [J]. Graefes Arch Clin Exp Ophthalmol, 2017, 255(11): 2199-2210.
[22] Everts B, Amiel E, Huang SC, et al. TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKvarepsilon supports the anabolic demands of dendritic cell activation [J]. Nat Immunol, 2014, 15(4): 323-332.
[23] Zhao L, Ma R, Zhang L, et al. Inhibition of HIF-1a-mediated TLR4 activation decreases apoptosis and promotes angiogenesis of placental microvascular endothelial cells during severe pre-eclampsia pathogenesis [J]. Placenta, 2019, 83: 8-16. doi:10.1016/j.placenta.2019.06.375.
[24] Chen W, Qian L, Wu F, et al. Significance of toll-like receptor 4 signaling in peripheral blood monocytes of pre-eclamptic patients [J]. Hypertens Pregnancy, 2015, 34(4): 486-494.
[25] 熊小琴, 布占红, 朱虹, 等. 部分与全部子宫内膜电切术治疗子宫腺肌症临床疗效观察[J]. 中国研究型医院, 2018, 5(5): 41-43. XIONG Xiaoqin, BU Zhanhong, ZHU Hong, et al. Clinical efficacy of partial and total endometrial resection for adenomyosis [J]. Journal of Chinese Research Hospitals, 2018, 5(5): 41-43.
[26] Pontis AD, Alterio MN, Pirarba S, et al. Adenomyosis: a systematic review of medical treatment [J]. Gynecol Endocrinol, 2016, 32(9): 696-700.
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