Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (4): 55-61.doi: 10.6040/j.issn.1671-7554.0.2021.0472

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Expressions and clinical significance of PARP-1 and HIF-1α in adenomyosis tissues and primary cells

XU Ge1, LI Qing1, ZHANG Cancan2, TIAN Yongjie1   

  1. 1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong, China;
    2. Department of Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, Shandong, China
  • Published:2022-04-22

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Abstract: Objective To explore the expressions and significance of poly(ADP-ribose)polymerase-1(PARP-1)and hypoxia inducible factor-1(HIF-1α)in adenomyosis(AM)tissues and normal endometrial tissues. Methods The expressions of PARP-1 and HIF-1α in the eutopic and ectopic endometria of women with AM and in normal endometria were detected with immunohistochemistry. The primary interstitial cells were extracted from the eutopic and ectopic endometria of patients with AM. After the expression of PARP-1 was knocked down, the change of HIF-1α expression was measured. Results Immunohistochemistry results showed that the expression of PARP-1 was higher in the eutopic(26/31 vs 4/10, P=0.006)and ectopic(24/31 vs 4/10, P=0.027)endometria of AM than in normal endometria; the expression of HIF-1α was higher in the eutopic(24/31 vs 3/10, P=0.006)and ectopic(23/31 vs 3/10, P=0.012)endometria of AM than in normal endometria. There was a positive correlation(r=0.357, P=0.038 in eutopic endometrial tissues; r=0.519, P=0.002 in ectopic endometrial tissues)between expressions of PARP-1 and HIF-1α, but there was no significant difference in the expressions of PARP-1(P=0.052)and HIF-1α(P=0.767)in the eutopic and ectopic endometria of adenomyosis. Western blotting results showed that the expression of PARP-1 in ectopic endometrial interstitial cells of AM was higher than that in eutopic endometrial interstitial cells(P=0.025), and PARP-1 was highly expressed in eutopic(P<0.001)and ectopic(P<0.001)endometrial interstitial cells of AM compared with normal endometrial interstitial cells. After PARP-1 knockdown, the expressionin eutopic endometria(t=4.786, P=0.003), ectopic endometria(t=9.458, P<0.001), and normal endometria(t=5.740, P=0.001)was significantly reduced, and the reduction of ectopic endometrial interstitial cells in AM was the most obvious. There was no significant difference in the expression of HIF-1α in eutopic and ectopic endometrial interstitial cells of AM(P=0.896), and the expression of HIF-1α in eutopic endometrial stromal cells(P<0.001)and ectopic endometrial stromal cells(P<0.001)was higher than that in normal endometrium interstitial cells. After PARP-1 knockdown, the expression of HIF-1α in eutopic endometria of AM(t=15.768, P<0.001), ectopic endometrial cells(t=3.459, P=0.025), and normal endometria(t=4.221 5, P=0.014)was significantly lower than before. Conclusion Both PARP-1 and HIF-1α may be involved in the development of AM, and the expression of PARP-1 may affect the expression of HIF-1α.

Key words: Adenomyosis, Poly(ADP-ribose)polymerase-1, Hypoxia inducible factor-1α, Poly(ADP-ribose)polymerase-1 inhibitor, Eutopic and ectopic endometria

CLC Number: 

  • R711.71
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