JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (10): 90-96.doi: 10.6040/j.issn.1671-7554.0.2017.157

Previous Articles    

Expression and significance of programmed death-1 and its ligand PD-L1 in adenomyosis

WU Lukanxuan, ZHU Shan, YU Jinxi, LÜ Chunzi, LI Mingjiang   

  1. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2017-02-22 Online:2017-10-10 Published:2017-10-10

PDF (PC)

165

Abstract: Objective To investigate the expression and significance of PD-1 and its ligand PD-L1 in adenomyosis. Methods The expression level of PD-1 and PD-L1 in normal endometria, eutopic and ectopic adenomyotic endometria were determined by immunohistochemistry and Western blotting(proliferative phase n=15; secretory phase n=15 in each group). In addition, the relationship between different expressions of PD-1/PD-L1 among groups and menstrual cycle was analyzed. Results PD-1/PD-L1 expressed in both normal and adenomyotic endometria of different menstrual cycles. In addition, expressions of PD-1/PD-L1 in both ectopic and eutopic adenomyotic endometria were upregulated(P<0.01). However, there was no difference between proliferative phase and secretory phase in PD-1/PD-L1 expression of eutopic adenomyotic endometria(P=0.12; P=0.45). Conclusion The expressions of PD-1 and PD-L1 are upregulated in adenomyotic endometria, suggesting that the PD-1/PD-L1 pathway may be involved in the immune dysfunctions and pathogenesis of adenomyosis.

Key words: Adenomyosis, Programmed cell death-ligand 1, Immunotolerance, Programmed death-1, Endometrium

CLC Number: 

  • R711.71
[1] 郎景和. 关于子宫内膜异位症的再认识及其意义[J]. 中国工程科学, 2009:137-142.
[2] Tremellen KP, Russell P. The distribution of immune cells and macrophages in the endometrium of women with recurrent reproductive failure. Ii: Adenomyosis and macrophages[J]. J Reprod Immunol, 2012, 93(1): 58-63.
[3] Wang F, Wen Z, Li H, et al. Human leukocyte antigen-g is expressed by the eutopic and ectopic endometrium of adenomyosis[J]. Fertil Steril, 2008, 90(5): 1599-1604.
[4] Yu JJ, Sun HT, Zhang ZF, et al. Il15 promotes growth and invasion of endometrial stromal cells and inhibits killing activity of nk cells in endometriosis[J]. Reproduction, 2016, 152(2): 151-160.
[5] O DF, Roskams T, Van den Eynde K, et al. The presence of endometrial cells in peritoneal fluid of women with and without endometriosis[J]. Reprod Sci, 2017, 24(2): 242-251.
[6] Okazaki T, Honjo T. The pd-1-pd-l pathway in immunological tolerance[J]. Trends Immunol, 2006, 27(4): 195-201.
[7] Francisco LM, Sage PT, Sharpe AH. The pd-1 pathway in tolerance and autoimmunity[J]. Immunol Rev, 2010, 236: 219-242. doi: 10.1111/j.1600-065X.2010.00923.x.
[8] Chikuma S. Basics of pd-1 in self-tolerance, infection, and cancer immunity[J]. Int J Clin Oncol, 2016, 21(3): 448-455.
[9] Sotnikova N, Antsiferova I, Malyshkina A. Cytokine network of eutopic and ectopic endometrium in women with adenomyosis[J]. Am J Reprod Immunol, 2002, 47(4): 251-255.
[10] 张爱荣, 吴继海, 胡本忠, 等. 子宫内膜异位症并不孕患者腹腔液及外周血中细胞因子的变化[J]. 山东大学学报(医学版), 2004, 42(4): 460-462. ZHANG Airong, WU Jihai, HU Benzhong, et al. Interleukin variation in peritoneal fluid and serum of patients with endometriosis-related infertility[J]. Journal of Shandong University(Health Sciences), 2004, 42(4): 460-462.
[11] 王立杰, 冷金花, 郎景和. IL-8在子宫内膜异位症发病中的作用[J]. 山东大学学报(医学版), 2004, 42(4): 456-459. WANG Lijie, LENG Jinhua, LANG Jinghe. Role of interleukine-8 in the pathogenesis of endometriosis[J]. Journal of Shandong University(Health Sciences), 2004, 42(4): 456-459.
[12] Nishimura H, Honjo T. Pd-1: An inhibitory immunoreceptor involved in peripheral tolerance[J]. Trends Immunol, 2001, 22(5): 265-268.
[13] Keir ME, Butte MJ, Freeman GJ, et al. Pd-1 and its ligands in tolerance and immunity[J]. Annu Rev Immunol, 2008, 26: 677-704. doi: 10.1146/annurev.immunol.26.021607.090331.
[14] Latchman Y, Wood CR, Chernova T, et al. Pd-l2 is a second ligand for pd-1 and inhibits t cell activation[J]. Nat Immunol, 2001, 2(3): 261-268.
[15] Scheerer C, Bauer P, Chiantera V, et al. Characterization of endometriosis-associated immune cell infiltrates(emaici)[J]. Arch Gynecol Obstet, 2016, 294(3): 657-664.
[16] Dong H, Strome SE, Salomao DR, et al. Tumor-associated b7-h1 promotes t-cell apoptosis: a potential mechanism of immune evasion[J]. Nat Med, 2002, 8(8): 793-800.
[17] Mazanet MM, Hughes CC. B7-h1 is expressed by human endothelial cells and suppresses t cell cytokine synthesis[J]. J Immunol, 2002, 169(7): 3581-3588.
[18] Kim S, Koh J, Kim MY, et al. Pd-l1 expression is associated with epithelial-to-mesenchymal transition in adenocarcinoma of the lung[J]. Hum Pathol, 2016, 58: 7-14. doi:10.1016/j.humpath.2016.07.007.
[19] Webb JR, Milne K, Kroeger DR, et al. Pd-l1 expression is associated with tumor-infiltrating t cells and favorable prognosis in high-grade serous ovarian cancer[J]. Gynecol Oncol, 2016, 141(2): 293-302.
[20] Madore J, Strbenac D, Vilain R, et al. Pd-l1 negative status is associated with lower mutation burden, differential expression of immune-related genes, and worse survival in stage-iii melanoma[J]. Clin Cancer Res, 2016, 22(15): 3915-3923.
[21] Madore J, Vilain RE, Menzies AM, et al. Pd-l1 expression in melanoma shows marked heterogeneity within and between patients: Implications for anti-pd-1/pd-l1 clinical trials[J]. Pigment Cell Melanoma Res, 2015, 28(3): 245-253.
[22] Merelli B, Massi D, Cattaneo L, et al. Targeting the pd1/pd-l1 axis in melanoma: biological rationale, clinical challenges and opportunities[J]. Crit Rev Oncol Hematol, 2014, 89(1): 140-165.
[23] Chang K, Svabek C, Vazquez-Guillamet C, et al. Targeting the programmed cell death 1: programmed cell death ligand 1 pathway reverses t cell exhaustion in patients with sepsis[J]. Crit Care, 2014, 18(1): R3. doi: 10.1186/cc13176.
[24] Yang L, Huang F, Mei J, et al. Posttranscriptional control of pd-l1 expression by 17beta-estradiol via pi3k/akt signaling pathway in eralpha-positive cancer cell lines[J]. Int J Gynecol Cancer, 2017, 27(2): 196-205.
[1] LIN Xueyan, LI Chunyan, HOU Xiaoman, TIAN Yongjie. Expressions of PARP-1 and EMT markers in eutopic and ectopic endometrium of adenomyosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 36-40.
[2] LIU Jing, ZHANG Hui, YAN Lei, ZHAO Xing-bo, LI Ming-jiang. Expression and significance of RhoE／Rnd3 protein in adenomyosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 116-.
[3] LAI Xiao-lan1, HUA Xing2, CHEN Yan-hui1. Expression of nerve fibers in the eutopic endometrium in endometriosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 107-110.
[4] REN Shao-da1, YU Hui2, MA Bao-hua1, LI Li-li1, HAO Ai-jun1, WANG Zhe-min3. Relationship between anti-implantation effect of arnebia root and expression of muc1 in the endometrium of early pregnant mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(9): 25-28.
[5] . Effect of  glutathione on proliferation of KLE and 
HEC1b endometrial cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(9): 58-61.
[6] DING Ning, BI Wen-Xiang, KONG Feng, ZHANG Pei-Hai. Antioxidant ability of type I and type II endometrial cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 63-66.
[7] . Expressions of PTEN and Akt and relationship to adenomyosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(10): 86-90.
[8] YU Qing-mei,WU Yu-ling,SONG Hai-yan,YIN Hua-wei,ZHUANG Yuan. Expression of p38 mitogen-activated protein kinase in early embryos and the peri-implantation endometrium in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(2): 123-127.
[9] LIAN Rui-qing,YU Hui,WANG Zhe-min,GU Xian-min,MA Bao-hua. Role of the Chinese drug saffron on the expression of leukemia inhibitory factor in the endometrium of early pregnant mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(1): 68-70.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd