Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (4): 30-37.doi: 10.6040/j.issn.1671-7554.0.2021.1108

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Deubiquitinase USP35 promotes the cells migration and invasion of non-small cell lung cancer

CHEN Zhaobo1, FANG Min2, XUE Haoran1, LIU Chunyan3   

  1. 1. Department of Clinical Laboratory, Qilu Hospital(Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao 266035, Shandong, China;
    2. Department of Gynaecology, Qingdao Women and Childrens Hospital, Qingdao 266034, Shandong, China;
    3. College of Basic Medicine, Qingdao University, Qingdao 266071, Shandong, China
  • Published:2022-04-22

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Abstract: Objective To investigate the effects of deubiquitinase ubiquitin-speci cproteases 35(USP35)on the migration and invasion of non-small cell lung cancer(NSCLC). Methods The protein expression of USP35 in a panel of 7 human NSCLC cell lines was examined with Western blotting. USP35 overexpression plasmids and control plasmids were transfected into H1299 cells, while USP35 knockdown plasmids and control plasmids were transfected into Anip973 cells according to Lipofectamine 2000 instructions. The migration of NSCLC cells was analyzed using Transwell blank chambers, and the invasion was analyzed using the Transwell chambers containing matrigel substrate gels. The protein expressions of E-cadherin and Vimentin were detected with Western blotting after USP35 overexpressing or silencing in aforementioned cell lines. Results Of the 7 NSCLC cell lines tested, USP35 expression was the lowest in H1299 cells, and the highest in Anip973 cells. Compared with transfected USP35 control plasmids, transfected USP35 overexpression plasmids had significantly increased migration and invasion, down-regulated E-cadherin, and up-regulated Vimentin expressions. Compared with transfected USP35 control plasmids, the Anip973 cells of transfected USP35 knockdown plasmids had significantly reduced migration and invasion, up-regulated E-cadherin, and down-regulated Vimentin expressions. Conclusion USP35 plays a critical role in the migration and invasion of NSCLC cells, hence may become a therapeutic option for NSCLC.

Key words: Ubiquitin-speciı, c proteases 35, Non-small cell lung cancer, Invasion, Migration, Epithelial-mesenchymal transition

CLC Number: 

  • R574
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