Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (4): 50-54.doi: 10.6040/j.issn.1671-7554.0.2021.1525

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Evaluation of CD4+CD25+FoxP3+ regulatory T cells and FoxP3 mRNA expression in 20 NMOSD patients

LI Honghao1, YU Jing2, CHEN Yali1, GUO Shougang1   

  1. 1. Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China;
    2. Department of Pathology and Pathophysiology, Shandong Medical College, Jinan, 250002, Shandong, China
  • Published:2022-04-22

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Abstract: Objective To assess the percentage of CD4+CD25+FoxP3+ regulatory T(Treg)cells and the mRNA expression of forkhead box protein 3(FoxP3)in patients with neuromyelitis optica spectrum disorders(NMOSD). Methods The case-control study compared 20 NMOSD patients with 20 healthy controls. The mononuclear cells in peripheral blood were collected. The percentage of CD4+CD25+FoxP3+ Treg cells was measured with flow cytometry using specific monoclonal antibodies recognizing CD4, CD25 and FoxP3 markers. FoxP3 gene expression was determined with real-time polymerase chain reaction. The expressions of transforming growth factor β1(TGF-β1), interferon γ(IFN-γ), and interleukin 10(IL-10)were determined with enzyme-linked immunosorbent assay. Results The percentage of CD4+CD25+FoxP3+ Treg cells and the mRNA expression of FoxP3 were significantly decreased in NMOSD patients compared with those in the controls(P<0.05). NMOSD patients had significantly increased IFN-γ(P<0.05)but decreased IL-10(P<0.05)levels, while there was no significant difference in the level of TGF-β1. Conclusion NMOSD may be associated with an abrogated function of circulating CD4+CD25+FoxP3+ Treg cells and a decreased level of FoxP3 gene expression.

Key words: Neuromyelitis optica spectrum disorders, CD4+CD25+FoxP3+ regulatory T cells, Forkhead box protein 3, Transforming growth factor-β1, Interferon-γ, Interleukin-10

CLC Number: 

  • R744.5
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