Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (3): 76-82.doi: 10.6040/j.issn.1671-7554.0.2021.0660

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LncRNA-UCA1 effects invasion and metastasis of trophoblast cells by targeting miR-182-5p

ZHONG Lili1, SHENG Ying1, GUO Jianghong1, YANG Shuangjian1, HE Yijing2   

  1. 1. The First Affiliated Hospital, Department of Obstetrics, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;
    2. The Second Affiliated Hospital, Department of Obstetrics and Gynecology, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China
  • Published:2022-03-09

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Abstract: Objective To investigate the effect of long non-coding RNA urethelial carcinoma related gene 1(LncRNA-UCA1)on invasion and metastasis of trophoblast cells and its possible mechanism. Methods Thirty placentas tissues from patients with preeclampsia(PE)and other 30 normal placentas tissues were collected respectively. The expression levels of UCA1 and miR-182-5p in placentas tissues were detected by qRT-PCR. UCA1 overexpression plasmid(pcDNA3.1-UCA1)and its negative plasmid(Vector)were transfected into HTR-8/Svneo cells respectively, and the expression levels of UCA1 and miR-182-5p in cells were detected by qRT-PCR. The targeted regulatory relationship between UCA1 and miR-182-5p was verified by dual-luciferase reporter assay. HTR-8/Svneo cells were transfected with pcDNA3.1-UCA1 plasmid and miR-182-5p mimic respectively or simultaneously, and then cell proliferation activity was detected by CCK-8. Cell invasion and metastasis was detected by Transwell assay. The expression levels of matrix metalloproteinase(MMP)-2 and MMP-9 proteins were detected by Western blotting. Results (1) Results of clinical specimen determination: compared with the placental tissues of normal pregnant women, the expression of UCA1 in placental tissues of PE patients was significantly decreased(1.10±0.51 vs 5.74±1.63, t=14.947, P<0.001), while the expression of miR-182-5p was significantly increased(7.23±1.44 vs 1.07±0.72, t=20.961,P<0.001). (2) Results of cell experiment: UCA1 could specifically target and regulate miR-182-5p expression. Compared with the Vector group, overexpression of UCA1 decreased the expression level of miR-182-5p in HTR-8/SVneo cells(0.12±0.05 vs 0.97±0.06, t=19.028, P<0.001), promoted the proliferation, invasion and migration of HTR-8/SVneo cells(t=10.160, 17.228, 13.768, all P<0.001), and up-regulated the expressions of MMP-2 and MMP-9 protein(t=20.833, 24.793, both P<0.001). However, the overexpression of miR-182-5p significantly reduced the promotion of UCA1 overexpression on the proliferation, invasion and migration of HTR-8/SVneo cells. Conclusion UCA1 promotes trophoblast invasion and metastasis by targeting the down-regulation of miR-182-5p.

Key words: Long non-coding RNA, Urethelial carcinoma related gene 1, Preeclampsia, Trophoblast cell, Invasion, Metastasis

CLC Number: 

  • R714
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