Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (4): 24-29.doi: 10.6040/j.issn.1671-7554.0.2021.1392

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Mouse breast cancer induces changes of the microenvironment in pre-metastatic lung tissue

ZHAO Tingting, QI Yana, ZHANG Ying, YUAN Bing, HAN Mingyong   

  1. Cancer Therapy and Research Center, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong, China
  • Published:2022-04-22

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Abstract: Objective To explore lung tissue changes and possible mechanisms before breast cancer metastasis. Methods A total of 20 female BALB/c mice were randomly divided into the experimental group and control group, with 10 mice in either group. Breast cancer cells were suspended in serum free medium and injected into the fourth pair of breast glands on the abdominal wall of mice in the experimental group, while equal amounts of PBS was injected into the same site in mice in the control group. On day 0, 7, 14, 21 and 28 after injection, 2 mice from both groups were sacrificed, their lung tissues were fixed with 4% paraformaldehyde, embedded in paraffin and sectioned. The histopathological changes were observed with HE staining; the expressions of fibronectin, type I collagen and CD31 were detected with immunohistochemistry and semi-quantitatively analyzed with Image-Pro Plus 6.0 software system. Results Compared with the control group, the experimental group had increased tumor volume but decreased weight. HE staining showed the number of inflammatory cells in lung tissue increased and the normal physiological structure was destroyed in the experimental group. Immunohistochemistry showed significant differences in the expressions of fibronectin, type Ⅰ collagen and CD31 between the two groups(all P<0.001). Conclusion In the pre-metastatic stage, malignant breast tumor can change the microenvironment of lung tissue, induce pulmonary inflammation response and promote the metastasis of tumor.

Key words: Breast cancer, Pre-metastatic, Lung, Type I collagen, Vessel, Animal model

CLC Number: 

  • R737.9
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