Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (1): 22-27.doi: 10.6040/j.issn.1671-7554.0.2020.1390

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Optimization of rat tympanosclerosis model and the correlation between tympanosclerosis and high expression of fibronectin

QIU Jingjing1, WU Ting1, MOU Yakui1, CUI Limei1, GUO Wentao1, HAN Fengchan2, SUN Yan1   

  1. 1. Department of Otorhinolaryngology and Head and Neck Surgery, Yuhuangding Hospital ofQingdao University, Yantai 26400, Shandong, China;
    2. Shandong Provincial Key Laboratory of Otological Genetic Diseases, Binzhou Medical University, Yantai 264003, Shandong, China
  • Published:2021-01-09

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Abstract: Objective To explore the optimization of tympanosclerosis model, and to investigate the expression of fibronectin and its role in the pathogenesis of tympanosclerosis. Methods A total of 60 Sprague-Dawley rats were randomly divided into 3 groups, with 20 in each group. In group A, neither ears were treated; in group B, Streptococcus pneumoniae was injected once into the right ears while the left ears were not treated; in group C, Streptococcus pneumoniae was injected twice into the right ears while the left ears were not treated. The formation of tympanosclerosis was observed with otomicroscope after 8 weeks. The morphological changes were observed with hematoxylin-eosin(HE)staining, the expression location of fibronectin was determined with immunohistochemical staining(IHC), and the expression level of fibronectin was detected with Western blotting. Results In group A, the tympanic membranes of all rats were normal, while in groups B and C, 35% and 78.9% of the tympanic membranes had tympanosclerosis. Obvious inflammatory infiltration and fibrous tissue hyperplasia could be seen in the middle ear mucosa of tympanosclerosis rats. Fibronectin was mainly located in submucosa. The expression level of fibronectin was 0.254±0.117, 1.154±0.163 and 1.111±0.622, respectively, in the three groups, with significant differences(F=6.768,P=0.006). Pairwise multiple comparison showed that there was significant difference in the expression of fibronectin among A1, B1 and C1 groups(P=0.013, P=0.018). Conclusion Two injections of Streptococcus pneumoniae can increase the success rate of modeling. Fibronectin is related to the pathogenesis of tympanosclerosis.

Key words: Tympanosclerosis, Animal model, Fibronectin

CLC Number: 

  • R764
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