JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 10-14.doi: 10.6040/j.issn.1671-7554.0.2014.440

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Pancreatic pathological and biochemical changes in a Bama miniature pig model of type 1 diabetes mellitus

YU Jian1, YE Yao2, HUANG Lili1, LIU Xiaoling1, MO Rufen1, YANG Fan1, HU Jing1, ZHOU Yingqiong3, HE Yongling4   

  1. 1. Department of Endocrinology, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China;
    2. Graduate School of Guilin Medical College, Guilin 541004, Guangxi, China;
    3. Department of Pathology, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China;
    4. Department of Clinical Laboratory, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China
  • Received:2014-07-07 Revised:2014-11-06 Published:2014-12-10

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Abstract: Objective To explore the efficacy of multiply intraperitoneal injection of streptozotocin (STZ) on establishment of type 1 diabetes mellitus (T1DM) model of Bama miniature pigs. Methods Twelve healthy male Bama miniature pigs were divided into control group (n=6) and diabetic group (n=6). Diabetic group was injected intraperitoneally with 75mg/kg STZ and one week later, with 150 mg/kg STZ. The control group was injected with the equal volume of sodium citrate solution. Fasting blood glucose was monitored by Roche glucose meter. Oral glucose tolerance test(OGTT)and insulin release test (IRT) were performed at the end of the experiment. Pancreatic tissues were collected for histopathological examination and β cell immunohistochemical staining. Results ① All miniature pigs in the diabetic group were the successful T1DM models and showed the characters of polyuria, polydipsia, polyphagia and weight loss. ② Since the 13th day after injection, fasting blood glucose of the diabetic group increased significantly and maintained at the level of (7.6-17.1) mmol/L. ③ One week after successful modeling, OGTT and IRT results indicated that the blood glucose level was higher and insulin was lower than the control group (all P<0.01). ④ The histopathological examination of pancreatic tissue pathology suggested that the numbers of pancreatic islets and β cells significantly decreased in the diabetic group. ⑤ Islet β cell immunohistochemical results suggested that the area of positive staining in the diabetic group was lower than that in the control group[(10.68±2.78)% vs (43.63±2.83)%, P<0.01]. Conclusion A new T1DM animal model is established by multiply intraperitoneal injection of STZ in Bama miniature pigs. The method has the advantages of simple operation, high success rate and safety.

Key words: Streptozotocin, Type 1 diabetes mellitus, Animal model, Bama miniature pig

CLC Number: 

  • R587.1
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