JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 5-9.doi: 10.6040/j.issn.1671-7554.0.2014.190

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Function of endogenous NT-3 in the regeneration sprouting of large neuron at late-stage spinal cord injury

WANG Xuyang1,2, JIANG Dunqing1, CHEN Shiwen1, TIAN Hengli1, LIU Fen2, LI Zhiqiang3   

  1. 1. Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai 200233, China;
    2. School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China;
    3. Department of Neurosurgery, South Campus of Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai 201499, China
  • Received:2014-04-03 Revised:2014-11-24 Published:2014-12-10

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Abstract: Objective To explore the function of endogenous neurotrophin-3 (NT-3) in the regeneration sprouting of large neuron at late-stage spinal cord following dorsal root rhizotomy in adult cats. Methods The 1st to the 5th lumbar (L1-L5), and the 7th lumbar (L7) to the 2nd sacral (S2) dorsal root ganlia (DRG) were exposed and removed from their respective intervertebral foramina, leaving the L6 DRG untouched. Antibody of NT-3 was blocked. Two months later, CB-HRP transganglionic tracing, histochemistry and enzymohistochemistry analysis were performed to compare the spinal cord segments and the spared DRG. Results NT-3 positive immunity reaction (IR) mainly distributed in large-sized neurons of DRG and part of neurons in spinal lamina II. Two months after the operation, the number of NT-3-IR neurons in DRG significantly increased (P<0.05), but in lamina Ⅱ there was no difference compared to the control group. After CB-HRP tetramethyl benzidine (TMB) enzymohistochemistry coloration, the densities of axon terminalsprouting derived from spared DRG were more markedly increased in the operation group than in the control group. However, after antibody blockade of NT-3, the densities decreased sharply compared to that of operation group. Conclusion The regeneration sprouting of large sensory neuron of DRG at late-stage distribute mainly in the deep layers (Ⅲ, Ⅳ, Ⅴ layer) of spinal dorsal horn. Endogenous NT-3 may be involved in the spinal cord plasticity by up-regulating the expression of NT-3, which promotes the long-term repair of injured spinal cord.

Key words: Morphological tracing, Large sensory neuron, Antibody blockade, Neural Regeneration, Neurotrophin-3

CLC Number: 

  • R323.85
[1] 王廷华, 吴良芳, 廖德阳, 等. 部分去背根猫备用背根节和脊髓II板层NT-3及其mRNA的表达变化[J]. 神经解剖学杂志, 2002, 18(1):59-62. WANG Tinghua, WU Liangfang, LIAO Deyang, et al. The change of NT-3 and its mRNA in spared dorsal root ganglion and spinal dorsal horn lamina Ⅱ following partial dorsal rhizotomy[J]. Chinese Journal of Neuroanatomy, 2002, 18(1):59-62.
[2] 周雪, 吴良芳, 谌宏鸣, 等. 猫脊髓部分去传入后神经营养素家族表达的时空变化[J]. 华西医科大学学报, 2002, 33(2): 165-168. ZHOU Xue, WU Liangfang, CHEN Hongming, et al. The spatiotemporal change of neurotrophin family and their mRNA expression in spinal cord of cats after partial rhizotomy[J]. J WCUMS, 2002, 33(2):165-168.
[3] 章为, 周雪, 王廷华, 等. 成年猫备用背根节内神经营养素-3对节内神经元的神经营养作用[J]. 四川大学学报: 医学版, 2004, 35(1): 25-28. ZHANG Wei, ZHOU Xue, WANG Tinghua, et al. The neurotrophic effect of endogenous NT-3 from adult cat spared dorsal root ganglion on ganglionic neurons [J]. J Sichuan Univ Med Sci Edi, 2004, 35(1):25-28.
[4] 刘芬, 张军明, 张禅, 等. 内源性神经营养因子-3在电针刺激治疗脊髓损伤中的作用[J]. 解放军医学杂志, 2009, 34(8):1000-1001. LIU Fen, ZHANG Junming, ZHANG Chan, et al. Effect of endogenous NT-3 on spinal cord injury treated with electro-acupuncture[J]. Med J Chin PLA, 2009, 34(8):1000-1001.
[5] Maisonpierre P C, Belluscio L, Squinto S, et al. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF[J]. Science, 1990, 247(4949):1446-1451.
[6] Schnell L, Schneider R, Kolbeck R, et al. Neurotrophin-3 enhances sprouting of corticospinal tract during development and after adult spinal cord lesion [J]. Nature, 1994, 367(6459):170-173.
[7] Ernfors P, Ibáez C F, Ebendal T, et al. Molecular cloning and neurotrophic activities of a protein with structural similarities to nerve growth factor: developmental and topographical expression in the brain [J]. Proc Natl Acad Sci U S A, 1990, 87(14): 5454-5458.
[8] 倪师今, 王廷华, 陈彦红. 外周支及中枢支切断对背根节BDNF和NT-3表达的影响[J]. 华西医科大学学报, 2001, 32(3): 350-352. NI Shijin, WANG Tinghua, CHEN Yanhong. The Expression of BDNF and NT-3 in dorsal root ganglion following peripheral and central axotomy—an immunohistochemical study[J]. J WCUMS, 2001, 32(3):350-352.
[9] Knüsel B, Hefti F. K-252b is a selective and nontoxic inhibitor of nerve growth factor action on cultured brain neurons [J]. J Neurochem, 1991, 57(3): 955-962.
[10] Blits B, Oudega M, Boer G J, et al. Adeno-associated viral vector-mediated neurotrophin gene transfer in the injured adult rat spinal cord improves hind-limb function[J]. Neuroscience, 2003, 118(1): 271-281.
[11] LIU C N, Chambers W W. Intraspinal sprouting of dorsal root axons; development of new collaterals and preterminals following partial denervation of the spinal cord in the cat[J]. AMA Arch Neurol Psychiatry, 1958, 79(1): 46-61.
[12] Li X L, Zhang W, Zhou X, et al. Temporal changes in the expression of some neurotrophins in spinal cord transected adult rats[J]. Neuropeptides, 2007, 41(3): 135-143.
[13] Li X L, Liu J, Wang X Y, et al. Temporal changes in the expression of TGF-beta 1 and EGF in the ventral horn of the spinal cord and associated precentral gyrus in adult Rhesus monkeys subjected to cord hemisection[J]. J Neurol Sci, 2008, 268(1-2): 163-171.
[14] Wang T H, Wang X Y, Li X L, et al. Effect of electroacupuncture on neurotrophin expression in cat spinal cord after partial dorsal rhizotomy[J]. Neurochem Res, 2007, 32(8):1415-1422.
[15] Wang X Y, Li X L, Hong S Q, et al. Electroacupuncture induced spinal plasticity is linked to multiple gene expressions in dorsal root deafferented rats[J]. J Mol Neurosci, 2009, 37(2): 97-110.
[16] Tafreshi A P, Zhou X F, Rush R A. Endogenous nerve growth factor and neurotrophin-3 act simultaneously to ensure the survival of postnatal sympathetic neurons in vivo[J]. Neuroscience, 1998, 83(2): 373-380.
[17] Freeman A Y, Pierce R C. Neutralization of neutrophin-3 in the ventral tegmental area or nucleus accumbens differentially modulates cocaine-induced behavioral plasticity in rats[J]. Neuroscience, 2002, 46(2): 57-65.
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