JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 30-34.doi: 10.6040/j.issn.1671-7554.0.2016.413

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Correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy

HU Yujing1, WU Dayong1, ZHANG Wenyan1, BIAN Yanzhu1, WEI Qiang1, TIAN Congna1, CHANG Shengli2   

  1. 1. Department of Nuclear Medicine, Hebei General Hospital, Shijiazhuang 050051, Hebei, China;
    2. Department of Surgery, Zhengding Peoples Hospital, Shijiazhuang 050800, Hebei, China
  • Received:2016-04-13 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective To explore the correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α(HIF-1α)expression in the hypoxic changes of tumors after radiotherapy. Methods The tumor-bearing mice were divided into radiotherapy groups(R12h, R24h, R36h)and control groups(C12h, C24h, C36h)according to the time of developing. The radiotherapy groups received an exposure dose of 15 Gy each time, while the control groups received no radiotherapy. After 99Tcm-MNLS hypoxic scintigraphy, the radioactivity ratio of tumor to non-tumor(T/NT)was calculated. The level of HIF-1α in tumors was evaluated. After that, the correlation between T/NT and HIF-1α level was analyzed. Results The T/NT and HIF-1α level in R12h group and R36h group were higher than those in the C12h group and C36h group(all P=0.000). There was no difference between the R24h and C24h groups(P=0.612, 0.731). The T/NT was positively correlated with HIF-1α level in the radiotherapy groups(r=0.793, P=0.003), as well as in the control groups(r=0.756, P=0.004). Conclusion Uptake of 99Tcm-MNLS and expression of HIF-1α are strongly 山 东 大 学 学 报 (医 学 版)55卷8期 -胡玉敬,等.放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性 \=-correlated to the changes of hypoxia in tumors after radiotherapy, and 99Tcm-MNLS hypoxic scintigraphy can provide the basis for the best time of reirradiation.

Key words: Hypoxia-inducible factor 1α, Radionuclide imaging, Hypoxia, Animal models

CLC Number: 

  • R445.5
[1] 蒋力扬, 孟雪, 于金明. 用分子影像指导肿瘤精准治疗[J]. 中华核医学与分子影像杂志, 2016, 36(1): 3-6. JIANG Liyang, MENG Xue, YU Jinming. Precision therapy of oncology by molecular imaging [J]. Chin J Nucl Med Mol Imaging, 2016, 36(1): 3-6.
[2] 查智豪, 汪建军, 朱霖, 等. 新型乏氧肿瘤显像剂99Tcm-MNLS、99Tcm-MLS的合成及其在小鼠体内的生物分布[J]. 同位素, 2009, 22(4): 197-203. ZHA Zhihao, WANG Jianjun, ZHU Lin, et al. Preparation of hypoxic imaging agents 99Tcm-MNLS and 99Tcm-MLS and their biodistribution in mice[J]. Journal of Isotopes, 2009, 22(4): 197-203.
[3] Koumenis C, Alarcon R, Hammond E, et al. Regulation of p53 by hypoxia: dissociation of transcriptional repression and apoptosis from p53-dependent transactivation[J]. Mol Cell Biol, 2001, 21(4): 1297-1310.
[4] Blesius A, Cassier PA, Bertucci F, et al. Neoadiuvant imatinib in patients with locally advanced non metastatic GIST in the prospective BFR 14 trial[J]. BMC Cancer, 2011, 11: 72. doi: 10.1186/1471-2407-11-72.
[5] 边艳珠, 胡玉敬, 吴大勇, 等. 99Tcm-MNLS乏氧显像评估肿瘤放疗后乏氧状态变化的实验研究[J].中华核医学与分子影像杂志, 2013, 33(5): 367-371. BIAN Yanzhu, HU Yujing, WU Dayon, et al. The assessment of tumor hypoxia variation after radiotherapy with 99Tcm-MNLS in mice tumor model[J]. Chin J Nucl Med Mol Imaging, 2013, 33(5): 367-371.
[6] 胡玉敬, 边艳珠, 吴大勇, 等. 99Tcm-MNLS与99Tcm-HL91乏氧显像评估肿瘤放疗后乏氧状态的对比实验研究[J]. 重庆医学, 2015, 44(19): 2618-2620. Hu Yujing, BIAN Yanzhu, ZHANG Wenyan, et al. Comparison between 99Tcm-MNLS and 99Tcm-HL91 in assessing the hypoxia of the tumor after radiotherapy[J]. Chongqing Yixue, 2015, 44(19): 2618-2620.
[7] Srinivasan A, Mohan S, Mukherji SK. Biologic imaging of head and neck cancer: the present and the future[J]. AJNR Am J Neuroradiol, 2012, 33(4): 586-594.
[8] Guan Y, Reddy KR, Zhu Q, et al. G-rich oligonucleotides inhibit HIF-1 alpha and HIF-2 alpha and block tumor growth[J]. Mol Ther, 2010, 18(1): 188- 197.
[9] Coothankandaswamy V, Liu Y, Mao SC, et al. The alternative medicine pawpaw and its acetogenin constituents suppress tumor angiogenesis via the HIF-1/VEGF pathway[J]. J Nat Prod, 2010, 73(5): 956- 961.
[10] Yang F, Zhang H, Mei Y, et al. Reciprocal regulation of HIF-1α and lincRNA-p21 modulates the Warburg effect[J]. Mol Cell, 2014, 53(1): 88-100.
[11] Reszec J, Rutkowski R, Chyczewski L. The expression of hypoxia-inducible factor-1 in primary brain tumors[J]. Int J Neurosci, 2013, 123(9): 657-662.
[12] Yorulmaz H, Ozkok E, Erguven M, et al. Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor1 α in hepatic tissue during early phase of sepsis[J]. Int J Clin Exp Med, 2015, 8(3): 3640-3650.
[13] Generali D, Berruti A, Cappelletti MR, et al. Effect of primary letrozole treatment on tumor expression of mTOR and HIF-1α and relation to clinical response[J]. J Natl Cancer Inst Monogr, 2015, 2015(51): 64-66.
[14] Clarke RH, Moosa S, Anzivino M, et al. Sustained radiosensitization of hypoxic glioma cells after oxygen pretreatment in an animal model of glioblastoma and in vitro models of tumor hypoxia[J]. PLoS One, 2014, 9(10): e111199.
[15] Fan G, Bo J, Wan R, et al. The effect of lentiviral vector-mediated RNA interference targeting hypoxia-inducible factor 1α on the uptake of fluorodeoxyglucose((18)f)in the human pancreatic cancer cell line, patu8988[J]. Cancer Biother Radiopharm, 2015, 30(4): 160-168.
[16] Zornhagen KW, Hansen AE, Oxboel J, et al. Micro regional heterogeneity of 64Cu-ATSM and 18F-FDG uptake in canine soft tissue sarcomas: relation to cell proliferation, hypoxia and glycolysis[J]. PLoS One, 2015, 10(10): e0141379. doi: 10.1371/journal.pone.0141379. eCollection 2015.
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