JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 30-34.doi: 10.6040/j.issn.1671-7554.0.2016.413

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Correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy

HU Yujing1, WU Dayong1, ZHANG Wenyan1, BIAN Yanzhu1, WEI Qiang1, TIAN Congna1, CHANG Shengli2   

  1. 1. Department of Nuclear Medicine, Hebei General Hospital, Shijiazhuang 050051, Hebei, China;
    2. Department of Surgery, Zhengding Peoples Hospital, Shijiazhuang 050800, Hebei, China
  • Received:2016-04-13 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective To explore the correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α(HIF-1α)expression in the hypoxic changes of tumors after radiotherapy. Methods The tumor-bearing mice were divided into radiotherapy groups(R12h, R24h, R36h)and control groups(C12h, C24h, C36h)according to the time of developing. The radiotherapy groups received an exposure dose of 15 Gy each time, while the control groups received no radiotherapy. After 99Tcm-MNLS hypoxic scintigraphy, the radioactivity ratio of tumor to non-tumor(T/NT)was calculated. The level of HIF-1α in tumors was evaluated. After that, the correlation between T/NT and HIF-1α level was analyzed. Results The T/NT and HIF-1α level in R12h group and R36h group were higher than those in the C12h group and C36h group(all P=0.000). There was no difference between the R24h and C24h groups(P=0.612, 0.731). The T/NT was positively correlated with HIF-1α level in the radiotherapy groups(r=0.793, P=0.003), as well as in the control groups(r=0.756, P=0.004). Conclusion Uptake of 99Tcm-MNLS and expression of HIF-1α are strongly 山 东 大 学 学 报 (医 学 版)55卷8期 -胡玉敬,等.放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性 \=-correlated to the changes of hypoxia in tumors after radiotherapy, and 99Tcm-MNLS hypoxic scintigraphy can provide the basis for the best time of reirradiation.

Key words: Hypoxia-inducible factor 1α, Radionuclide imaging, Hypoxia, Animal models

CLC Number: 

  • R445.5
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