JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 11-15.doi: 10.6040/j.issn.1671-7554.0.2015.513

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Chronic intermittent hypobaric hypoxia enhances the vasodilatation of thoracic aorta via PI3K-dependent eNOS activation in rats

WANG Lixuan1*, ZHANG Lu2*, XU Xin2, LI Sixue2, LIU Min3, WANG Yaping3, MA Huijuan3,4   

  1. 1. Department of Histology and Embryology;
    2. Class of Seven-year Clinical Medicine Registered in 2011;
    3. Department of Physiology;
    4. Hebei Key Laboratory of Laboratory Animal Science, Hebei Medical University, Shijiazhuang 050017, Hebei, China
  • Received:2015-05-24 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To investigate the effects of chronic intermittent hypobaric hypoxia(CIHH)on the vasodilatation in isolated thoracic aorta and the nitric oxide related mechanism in rats. Methods A total of 80 male adult Sprague-Dawley rats were randomly divided into two groups: control group(CN, n=40)and CIHH group(CIHH, n=40). The rats in CIHH group were exposed to hypoxia simulating at 5000-meter altitude in a hypobaric chamber(PB=404 mmHg, PO2=84 mmHg)for 28 days, 6 hours each day. The rats in CN group lived in a normoxic environment for the same period. The vasodilatation of thoracic aorta was recorded by using organ bath technique. The protein expressions of eNOS and PI3K were measured by using Western blotting. Results CIHH could remarkably augment the acetylcholine(ACh)-induced vasodilatation of thoracic aorta(P<0.05)and increase the expression of eNOS in thoracic aorta tissues(P<0.05). Incubation with MEK inhibitor PD98059 did not affect the effects of CIHH on thoracic aorta. Incubation of PI3K inhibitor LY294002 blocked the effects of CIHH(P<0.05). Furthermore, CIHH treatment could improve the expression of PI3K in thoracic aorta tissue(P<0.05). Conclusion CIHH treatment enhances Ach-induced vasodilatation of thoracic aorta by activating eNOS via PI3K pathway.

Key words: PI3K, Anti-hypertension, Vasodilatation, Chronic intermittent hypobaric hypoxia, eNOS

CLC Number: 

  • Q463
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