诱导型一氧化氮合成酶参与大鼠心脏缺血后处理延迟相的保护作用

doi:10.6040/j.issn.1671-7554.0.2015.829

Abstract

Abstract: Objective To investigate the delayed cardioprotective effects of ischemic preconditioning(IPO)and the role of inducible nitric oxide synthase(iNOS)in the late phase of IPO. Methods A total of 160 rats were randomized into 5 groups: I/R group, IPO group, IPO+1400W(iNOS inhibitor)group, I/R+1400W group and sham-operated(sham)group. Animals in each group received 3, 24, 48 or 72 h of reperfusion after ischemia(n=8 per each time point). Cardio-infarct size and creatine kinase(CK)activity were measured respectively. Western blotting was used to assess the expressions of phosphorylated endothelial nitric oxide synthase(p-eNOS)and iNOS in the heart. Results At reperfusion 3 h, in the IPO group, the infarct size of left ventricle(LV)decreased significantly compared with I/R group［(18.0±2.3)% vs(30.7±3.1)%, P<0.05). At reperfusion 72 h, the difference between IPO group and I/R group in infarct size was obvious［(25.7±1.1)% vs(34.9±0.8)%, P<0.05］. Difference in the CK activity was positively correlated with the difference in infarct size in all groups. IPO increased p-eNOS levels at R 3 h and R 24 h 山东大学学报 (医学版)54卷2期 -李欣,等.诱导型一氧化氮合成酶参与大鼠心脏缺血后处理延迟相的保护作用 \=-and iNOS levels at R 48 h and R 72 h. Conclusion IPO has delayed cardioprotective effects on myocardial infarction, and iNOS plays a critical role in the delayed effects against heart injury.

Key words: Ischemic postconditioning, Ischemia-reperfusion injury, Endothelial nitric oxide synthase, Inducible nitric oxide synthase, Delayed cardioprotective effect

CLC Number:

R654

LI Xin, WANG Gongming, WANG Hong, WANG Yan, ZHANG Ligong, ZHANG Le, LIU Bei, ZHANG Mengyuan. Inducible nitric oxide synthase contributes to the delayed cardioprotection of ischemic postconditioning in rats[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 16-20.

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Inducible nitric oxide synthase contributes to the delayed cardioprotection of ischemic postconditioning in rats

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