JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (7): 6-11.doi: 10.6040/j.issn.1671-7554.0.2017.089

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Cardioprotective effects of allicin postconditioning on myocardial ischemia-reperfusion injury in rats

LIU Fang, QIAO Lei, CHEN Wenchao, YANG Lina   

  1. Department of Physiology, Henan Medical College, Zhengzhou 451191, Henan, China
  • Received:2017-01-21 Online:2017-07-10 Published:2017-07-10

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Abstract: Objective To explore the effects of allicin postconditioning on myocardial ischemia-reperfusion injury in rats. Methods A total of 18 Wistar rats were randomly allocated into 3 groups: ischemia reperfusion group(IR group), ischemic postconditioning group(IPO group)and allicin postconditioning group, with 6 rats in each group. Langendorff perfusion apparatus was used to build an isolated rat myocardial IR model. Isolated rat hearts were subjected to 30 min global ischemia followed by 60 min reperfusion after 20 min equilibrium. The left ventricular develop pressure(LVDP), the maximal ventricular pressure rise ratio during systolic period(+dp/dtmax), the maximal ventricular pressure decrease ratio during diastolic period(-dp/dtmax)and heart rate(HR)were recorded at the end of 20 min equilibrium and 60 min reperfusion separately. Heart perfusion transudate at the end of 20 min equilibrium and 60 min reperfusion were assessed to determine the activity of lactate dehydrogenase(LDH). Myocardium of left ventricle was detected to examine the activity of superoxide dismudase(SOD)and the contents of malondialdehyde(MDA). Results There was no difference in equilibrium hemodynamics observed among the groups(P>0.05). At the end of reperfusion, compared with IR group, the allicin postconditioning group showed significantly ameliorated functional recovery, 山 东 大 学 学 报 (医 学 版)55卷7期 -刘芳,等.大蒜素后处理对大鼠心肌缺血-再灌注损伤的保护作用 \=-decreased activity of LDH, enhanced activity of SOD, and decreased contents of MDA(all P<0.05). At the end of reperfusion, HR was obviously slower in the allicin postconditioning group than in the IPO group(P<0.001). There was no statistically significant difference between IPO group and allicin postconditioning group in other indicators(P>0.05). Conclusion Allicin administration at the beginning of reperfusion can significantly reduce ischemia-reperfusion injury and improve cardiac function, with similar cardioprotective effect as IPO.

Key words: Allicin postconditioning, Ischemic postconditioning, Myocardium, Rat, Ischemia-reperfusion injury

CLC Number: 

  • R654.2
[1] Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium[J]. Circulation, 1986, 74(5): 1124-1136.
[2] Zhao ZQ, Corvera JS, Halkos ME, et al. Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning[J]. Am J Physiol Heart Circ Physiol, 2003, 285(2): 579-588.
[3] Tsang A, Hausenloy DJ, Mocanu MM, et al. Postconditioning: a form of “modified reperfusion” protects the myocardium by activating the phosphatidylinositol 3-kinase-Akt pathway[J]. Circ Res, 2004, 95(3): 230-232.
[4] Kim MK, Choi WY, Lee HY. Enhancement of the neuroprotective activity of Hericium erinaceus mycelium co-cultivated with Allium sativum extract[J]. Arch Physiol Biochem, 2015, 121(1):19-25.
[5] Chan JY, Yuen AC, Chan RY, et al. A review of the cardiovascular benefits and antioxidant properties of allicin[J]. Phytother Res, 2013, 27(5): 637-646.
[6] Borlinghaus J, Albrecht F, Gruhlke MC, et al. Allicin: chemistry and biological properties[J]. Molecules, 2014, 19(8): 12591-12618.
[7] Zhang X, Zhu Y, Duan W, et al. Allicin induces apoptosis of the MGC-803 human gastric carcinoma cell line through the p38 mitogen-activated protein kinase/caspase-3 signaling pathway[J]. Mol Med Rep, 2015, 11(4): 2755-2760.
[8] 杨军岭, 杨侠. 大蒜素对6-羟多巴胺所致PC12细胞损伤的保护作用及机制[J]. 中华神经外科疾病研究杂志, 2015, 14(3): 208-212. YANG Junling, YANG Xia. Protection of allicin on PC12 cells injury induced by 6-OHDA in vitro[J]. Chinese Journal of Neurosurgical Disease Research, 2015, 14(3): 208-212.
[9] 蔡青, 秦怀洲, 陈昆仑. 大蒜素对脑胶质瘤细胞U87细胞侵袭能力的影响及其机制[J]. 西安交通大学学报(医学版), 2015, 36(2): 271-274. CAI Qing, QIN Huaizhou, CHEN Kunlun. The effect and mechanism of allicin on the migration and invasion properties of human osteosarcoma U87 cells[J]. Journal of Xi'an Jiaotong University(Medical Sciences), 2015, 36(2): 271-274.
[10] 李卿慧, 刘亚贤. 大蒜素在心血管方面保护作用研究进展[J]. 实用心脑肺血管病杂志, 2013, 21(10): 3-6.
[11] Chan JY, Tsui HT, Chung IY, et al. Allicin protects rat cardiomyoblasts(H9c2 cells)from hydrogen peroxide-induced oxidative injury through inhibiting the generation of intracellular reactive oxygen species[J]. Int J food Sci and Nutr, 2014, 65(7): 868-873.
[12] 鲁锐, 项标, 刘静文, 等. 大蒜素的临床应用研究进展[J].时珍国医国药, 2013, 24(3): 711-713.
[13] Li XH, Li CY, Xiang ZG, et al. Allicin ameliorates cardiac hypertrophy and fibrosis through enhancing of Nrf2 antioxidant signaling pathways[J]. Cardiovasc Drug Ther, 2012, 26(6): 457-465.
[14] 丁海丽, 苏全生. 大蒜素预处理对运动大鼠心肌细胞凋亡的影响[J]. 中国康复医学杂志, 2015, 30(7): 651-654. DING Haili, SU Quansheng. Effects of preceding Allicin on cardiomyocyte apoptosis in rat after exercise[J]. Chinese Journal of Rehabilitation Medicine, 2015, 30(7): 651-654.
[15] 谢敏, 谷万里. 大蒜素调节ApoE-/-小鼠心肌细胞凋亡及稳定易损斑块的作用机制[J]. 山东医药, 2017, 57(1): 29-31.
[16] 吴建伟, 罗春丽, 李家彬, 等. 大蒜素及缺血预处理对大鼠肾缺血再灌注损伤的影响及机制研究[J]. 重庆医科大学学报, 2010, 35(4): 549-552. WU Jianwei, LUO Chunli, LI Jiabin, et al. Effect of allitride injection and ischemic preconditioning on renal ischemia/reperfusion injury in rats[J]. Journal of Chongqing Medical University, 2010, 35(4): 549-552.
[17] 傅俊英, 刘秀华, 史载祥, 等. 大蒜素预处理对乳鼠心肌细胞缺氧复氧延迟保护作用的研究[J]. 中日友好医院学报, 2003, 17(3): 159-162. FU Junying, LIU Xiuhua, SHI Zaixiang, et al. Delayed cardioprotective effect of Allitridum on neonatal rat myocardiocytes subjected to hypoxia/ reoxygenation(H/R)[J]. Journal of China-Japan Friendship Hospital, 2003, 17(3): 159-162.
[18] 马丽娜, 李峰杰, 陈坚, 等. 大蒜主要活性成分及药理作用研究进展[J]. 中国药理学通报, 2014, 30(6): 760-763. MA Lina, LI Fengjie, CHEN Jian, et al. Research advances in garlics main active ingredients and their pharmacological effects[J]. Chinese Pharmacological Bulletin, 2014, 30(6): 760-763.
[19] García-Trejo EM, Arellano-Buendía AS, Argüello-García R, et al. Effects of allicin on hypertension and cardiac function in chronic kidney disease[J]. Oxidative Medicine & Cellular Longevity, 2016, 2016, Article ID 3850402, 13 pages.
[20] Chen S, Tang Y, Qian Y, et al. Allicin prevents H2O2-induced apoptosis of HUVECs by inhibiting an oxidative stress pathway[J]. BMC Complementary & Alternative Medicine, 2013, 14(1): 321-328.
[21] Bhardwaj K, Verma MK, Verma N, et al. Effect of long term supplementation of active garlic allicin in reducing blood pressure in hypertensive subjects[J]. Int J Adv Med, 2015, 2(3): 231-234.
[22] 李艳, 陈震. PI3K-Akt信号通路及β2-AR蛋白在外源性硫化氢后处理减轻大鼠离体心肌缺血再灌注损伤中的作用[J]. 中国现代医药杂志, 2014, 16(9): 1-5. LI Yan, CHEN Zhen. Exogenous hydrogen sulfide postconditioning attenuates myocardial injury in isolated rat hearts after ischemia/reperfusion via PI3K-Akt signaling pathway and modulation of β2-AR protein[J]. Modern Medicine Journal of China, 2014, 16(9): 1-5.
[23] 王会玲, 龚理, 习凤霞, 等. 大蒜素对缺血再灌注心脏血流动力学的影响[J]. 时珍国医国药, 2012, 23(9): 2343-2344.
[24] 程伟, 喻荣辉, 屈松柏. 大蒜素对豚鼠心房肌电生理的作用[J]. 中国中医基础医学杂志, 2002, 7(8): 35-37. CHENG Wei, YU Ronghui, QU Songbai. Effect of Allitridi on electrophysiological characteristic of guinea pig atrium muscles[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine, 2002, 7(8): 35-37.
[25] 邓春玉, 庄宁宁, 单志新, 等. 大蒜素对大鼠心室肌细胞L-型钙通道的影响[J].中药药理与临床, 2005, 21(3): 18-20. DENG Chunyu, ZHUANG Ningning, SHAN Zhixin, et al. Effect of allitridi on calcium current in rat ventricular myocytes[J]. Pharmacology and Clinics of Chinese Materia Medica, 2005, 21(3): 18-20.
[26] Yadav RK, Verma NS. Effects of garlic(Allium sativum)extract on the heart rate, rhythm and force of contraction in frog: a dose-dependent study[J]. Indian J Exp Biol, 2004, 42(6): 628-631.
[27] 余宇龙, 余刚, 杨娇娇, 等. 磷酸肌酸辅助治疗新生儿窒息合并重度心肌损害的效果及对心肌酶谱的影响[J]. 儿科药学杂志, 2017, 23(1): 17-19. YU Yulong, YU Cang, YANG Jiaojiao, et al. Phosphocreatine on myocardial enzymes in neonatal asphyxia with severe myocardial damage[J]. Journal of Pediatric Pharmacy, 2017, 23(1): 17-19.
[28] 丁海丽.大蒜素预处理对大鼠大负荷运动后及恢复期血液抗氧化应激能力的影响[J]. 天然产物研究与开发, 2016, 28(10): 1639-1642. DING Haili. Effect of allicin pretreatment on the ability of blood antioxidation of rats after heavy load exercise and during recovery period[J]. Natural Product Research and Development, 2016, 28(10): 1639-1642.
[29] 展宏刚, 孙淑娟, 梁孝印, 等. 大蒜素对大鼠心肌缺血的保护作用及相关机制研究[J]. 中国生化药物杂志, 2015, 35(1): 74-76. ZHAN Honggang, SUN Shujuan, LIANG Xiaoyin, et al. Protective effects of allicin on myocardial ischemia in rats and related mechanisms[J]. Chinese Journal of Biochemical Pharmaceutics, 2015, 35(1): 74-76.
[30] 于洋, 刘师兵, 李松岩, 等. 大蒜素对衰老模型小鼠心脏和肺的保护作用[J]. 医药导报, 2016, 35(2): 115-120. YU Yang, LIU Shibing, LI Songyan, et al. Protective Effects of Allicin on Heart and Lung in Aging Mice[J]. Herald of Medicine, 2016, 35(2):115-120.
[31] Zhu JW, Chen T, Guan J, et al. Neuroprotective effects of allicin on spinal cord ischemia-reperfusion injury via improvement of mitochondrial function in rabbits[J]. Neurochemistry International, 2012, 61(5): 640-648.
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