JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (7): 1-5.doi: 10.6040/j.issn.1671-7554.0.2016.1301

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A reliable system to assess atherosclerosis model of Bama minipigs

YIN Ni1, YANG Guanlin1, JIANG Junwen2, WANG Chuntian3, WANG Fengyao4, JIA Lianqun5, GAO Xiaoyu1, PAN Jiaxiang1, LI Qin1, LI Jia1, FENG Yuanjie1, GAO Yuzhu1, ZHOU He1, ZHANG Zhe1   

  1. 1. Blood-vessel Laboratory, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    2. Department of Cardiology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    3. Laboratory Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China;
    4. Department of Anesthesia, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    5. Platform of Scientific Research, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China
  • Received:2016-10-13 Online:2017-07-10 Published:2017-07-10

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Abstract: Objective To evaluate the feasibility, stability and safety of a coronary atherosclerosis model of Bama minipigs. Methods Bama minipigs were randomly assigned into control group(basic diet)and model group(high-fat diet combined with mechanical injury). In week 0, 24 and 48, both groups underwent coronary angiography, intravascular ultrasound(IVUS), electrocardiogram, serum lipid testing, inflammation factors testing, and pathological analysis 山 东 大 学 学 报 (医 学 版)55卷7期 -尹妮,等.巴马小型猪冠状动脉粥样硬化模型的评价方法 \=-to evaluate the model. Results In week 24, compared with the control group, the model group had significantly increased total cholesterol(TC), triglycerides(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), oxidized low-density lipoprotein(ox-LDL), high-sensitivity C-reactive protein(hs-CRP), interleukin-6(IL-6), vascular cell adhesion molecule-1(VCAM-1), monocyte chemotactic protein(MCP-1)and soluble P-selectin(SP-selectin)(P<0.05). There was no statistically significant difference between the two groups in ST segment depression in week 0 and 24(P>0.05). There was no statistically significant difference between the two groups in T waves in week 0(P>0.05), but there was statistically significant difference in T waves in week 24(P<0.05). In week 24 and 48, the model group showed significantly higher coronary stenosis rate than the control group. In week 48, the model group had higher intima/intima-medial thickness ratio than in week 24. Conclusion The in vitro and in vivo model is safe and feasible in the assessment of atherosclerosis.

Key words: Atherosclerosis, Bama minipigs, High-fat diet, Model evaluation, Mechanical injury, Animal model

CLC Number: 

  • R332
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