JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 5-9.doi: 10.6040/j.issn.1671-7554.0.2015.088

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The inhibitory effect of baicalin on endothelial cell apoptosis induced by ox-LDL

YU Xin, LIU Xiaojing, LIU Xiangqun   

  1. Department of Cadre Care, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-01-25 Revised:2015-03-11 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To explore the possible mechanism of baicalin on the apoptosis of endothelial cells in the development of atherosclerosis (AS). Methods Endothelial cells were extracted from Wistar rats' thoracic aorta and divided into normal control group, baicalin treatment group, and ox-LDL treatment group. The control group was cultured with 10% FBS ECM, while the baicalin treatment group, and ox-LDL treatment group were cultured with different concentrations of baicalin and ox-LDL (10, 25, 50 μg/mL) respectively for 24 hours. The apoptosis of cells was determined with flow cytometry, and expressions of Bax, Bcl-2 and Caspase-3 mRNA were measured with RT-PCR. After that, cells were treated with baicalin plus ox-LDL(50 μg/mL) for 24 hours and the above indexes were measured. Results After baicalin treatment, Bcl-2 mRNA expression increased (P<0.01), while Caspase-3, Bax mRNA and Bax/Bcl-2 ratio decreased (all P<0.01). Baicalin significantly inhibited apoptosis of endothelial cells in a concentration-dependent manner (P<0.01). Compared with the control group and ox-LDL group of the same concentration, cells treated with baicalin had markedly elevated expressions of Bcl-2 mRNA, Bax and Caspase-3 and Bax/Bcl-2 ratio. Compared with cells treated with ox-LDL, cells treated with baicalin plus ox-LDL had significantly reduced apoptosis rate (P<0.01). Conclusion Baicalin can prevent cell damage caused by ox-LDL by upregulating expression of Bcl-2 and downregulating expressions of Caspase-3 and Bax.

Key words: Apoptosis, Baicalin, Caspase-3, Atherosclerosis

CLC Number: 

  • R541.4
[1] Guo J, Li HZ, Zhang WH, et al. Increased expression of calcium-sensing receptors induced by ox-LDL amplifies apoptosis of cardiomyocytes during simulated ischaemia-reperfusion[J]. Clin Exp Pharmacol Physiol, 2010, 37(3):128-135.
[2] 辛文妤,宋俊科,何国荣,等.黄芩素和黄芩苷的药理作用及机制研究进展[J].中国新药杂志,2013,22(6):647-653. XIN Wenyu, SONG Junke, HE Guorong, et al. Progress in pharmacological study and the underlying mechanism of baicalein and baicalin[J]. Chinese Journal of New Drugs, 2013, 22(6):647-653.
[3] Dal Santo S, Stampfl H, Krasensky J, et al. Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis[J]. Plant Cell, 2012, 24(8):3380-3392.
[4] Tsukaharas S, Yamamoto S, Shew Tr, et al. Inhalmion of low-level formal-chehyde increases the bcl-2/bax expression ratio in the hippocam-pus of immunologically sensitized mice[J]. Neuroimmunomodulation, 2006, 13 (2):63-68.
[5] Guo J, Zhang K. Effects of ethyl pyruvate on myocardial apoptosis and expression of Bcl-2 and Bax Proteins after ischemia repeffusionin rats[J]. Huazhong Univ Sci Technol Med Sci, 2008, 28(3):281-283.
[6] Lin CS, Lin FY, Ho LJ. PKC signalling regulates SR-A and CD36 expression and foam cell formation[J]. Cardiovasc Res, 2012, 95(3):346-355.
[7] Chai YC, Binion DG, Macklis R, et al. Smooth muscle cell proliferation induced by oxidized LDL-borne lysophosphatidylcholine. Evidence for FGF-2 release from cells not extracellular matrix[J]. Vascul Pharmacol, 2002, 38(4):229-237.
[8] Hopkins PN. Molecular biology of atherosclerosis[J]. Physiol Rev, 2013, 93(3):1317-1542.
[9] Ait-Oufella H, Taleb S, Mallat Z. Recent advances on the role of cytokines in atherosclerosis[J]. Arterioscler Thromb Vasc Biol, 2011, 31(5):969-979.
[10] Bae YS, Lee J H, Choi SH, et al. Macrophages generate reactive oxygen species in response to minimally oxidized low-density lipoprotein:toll-like receptor 4-and spleen tyrosine kinase-dependent activation of NADPH oxidase 2[J]. Circ Res, 2009, 104(2):210-218.
[11] Li Y, Duan Z, Gao D, et al. The new role of LOX-1 in hypertension induced neuronal apoptosis[J]. Biochem Biophys Res Commun, 2012, 425(4):735-740.
[12] Gerry AB, Leake DS. A moderate reduction in extracellular pH protects macrophages against apoptosis induced by oxidized low density lipoprotein[J]. J Lipid Res, 2008, 49(4):782-789.
[13] Ding Z, Wang X, Khaidakov M, et al. Degradation of heparan sulfate proteoglycans enhances oxidized-LDL-mediated autophagy and apoptosis in human endothelial cells[J]. Biochem Biophys Res Commun, 2012, 426(1):106-111.
[14] Mitra S, Khaidakov M, Lu J, et al. Prior exposure to oxidized low-density lipoprotein limits apoptosis in subsequent generations of endothelial cells by altering promoter methylation[J]. Am J Physiol Heart Circ Physiol, 2011, 301(2):H506-H513.
[15] 高爱社, 沈晓君. 黄芩苷对氧化低密度脂蛋白诱导的血管内皮细胞凋亡的防护作用[J].世界中医药, 2009, 4(5):289-290. GAO Aishe, SHEN Xiaojun. Protective effect of bacilin on apoptosis of vascular endothelial cells induced by ox-LDL[J].World Chinese Medicine, 2009, 4(5):289-290.
[16] 孙洁, 田林红, 王收宝. 黄芩苷对糖尿病大鼠肾脏细胞凋亡及其Bcl-2和Bax表达的影响[J]. 实用医学杂志, 2011, 27(5):757-760. SUN Jie, TIAN Linhong, WANG Shoubao. Effect of bacilin on apoptosis and experssion of Bax and Bcl-2 in the kindy of diabetic rats[J]. The Journal of Practial Medicine, 2011, 27(5):757-760.
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