JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (2): 68-73.doi: 10.6040/j.issn.1671-7554.0.2015.486

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Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells

TU Yunhua1, CHEN Liyuan1, WANG Xi1, ZHOU Ying2, KANG Yingqian3, XUE Yuecui4, RONG Dongyun4, YE Zhenyuan4, CAO Yu4   

  1. 1. Department of Dermatology, the Second Peoples Hospital of Guiyang City, Guiyang 550081, Guizhou, China;
    2. Guizhou Province Chinese Medicine and Ethnic Medicine Creation Engineering Center, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China;
    3. Department of Microbiology, Guizhou Medical University, Guiyang 550004, Guizhou, China;
    4. Department of Dermatology and Venereal Disease, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2015-05-14 Online:2017-02-10 Published:2017-02-10

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Abstract: Objective To investigate the effect of ar-turmerone derivatives(ATD)on proliferation and apoptosis in human melanoma WM35 cells, and to explore its mechanism. Methods WM35 cells were incubated with different concentrations of ATD(5-80 μmol/L)in vitro. Cell proliferation was measured by cell counting kits(CCK-8)assay. The cell morphology of WM35 was observed by inverted microscope after AO/EB staining. Apoptosis was detected by DNA fragmentation. Caspase-3 cellular activities were measured by a colorimetric method. The apoptosis rate was analyzed by flow cytometry. The expression of apoptosis associated protein, Bcl-2 and Bax, in WM35 cells were examined by 山 东 大 学 学 报 (医 学 版)55卷2期 -涂云华,等.芳姜黄酮衍生物对WM35细胞增殖及凋亡的影响 \=-Western blotting. Results ATD exhibited obvious proliferation inhibition effect on the growth of WM35 cells in a time-and-dose dependent manner(P<0.05). Meanwhile, ATD exhibited an apoptosis-inducing effect on WM35 cells in a drug-and-dose dependent manner(P<0.05). Expression of Bax increased while Bcl-2 decreased with the increase of the concentration of ATD(P<0.05). Conclusion ATD exhibites marked effect of proliferation inhibition and apoptosis-inducing on WM35 cells. ATD activates the apoptosis pathway of key enzyme. The apoptosis associated protein Bax is up-regulated while that of Bcl-2 is down-regulated, which may be the mechanism of ATD in affecting the proliferation and differentiation of tumors.

Key words: WM35 cells, Apoptosis, Ar-turmerone derivatives

CLC Number: 

  • R739.5
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