JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 38-42.doi: 10.6040/j.issn.1671-7554.0.2015.828

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Expression of apoptosis-related genes in peripheral blood mononuclear cells of patients with HBV infection and the relationship with chronicity of hepatitis B

ZHANG Kun, ZHAGN Xiaoguo, CUI Xingxing, CHEN Shijun   

  1. The Department of Liver Diseases, Jinan Infectious Hospital, Jinan 250021, Shandong, China
  • Received:2015-09-01 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To investigate the different apoptosis pathways of peripheral blood lymphocytes cells(PBMCs)and their roles in the chronicity of HBV infection. Methods The study included 9 chronic asymptomatic HBV carriers(ASC), 48 patients with chronic hepatitis B(CHB), 24 patients with liver cirrhosis(LC)and 20 normal controls(NC). The mRNA expressions of apoptosis-related genes(FAS、CASP8、CYCS、CASP9、CASP3)in PBMCs were examined with real time fluorescent quantitative PCR, and the protein expressions in plasma were detected with enzyme linked immunosorbent assay(ELISA). Results The mRNA expressions of apoptosis-related genes were higher in CHB and ASC groups, as compared to NC and LC groups(P<0.05); there was no difference between CHB group and ASC group(P>0.05); the expressions were higher in LC group than in NC group(P<0.05). The protein expressions of apoptosis-related genes in CHB and ASC groups were higher than in NC and LC groups(P<0.008 3); there was no difference between CHB and ASC groups(P>0.008 3); the expressions were higher in LC group than in NC group(P<0.008 3). Conclusions HBV infection can induce the apoptosis of PBMCs, with extrinsic and intrinsic pathways involved. The apoptosis of PBMCs plays an important role in the chronicity of HBV infection.

Key words: Chronic hepatitis B, Peripheral blood mononuclear cell, Apoptosis-related gene, Cirrhosis, Enzyme linked immunosorbent assay, Real time quantitative PCR

CLC Number: 

  • R511
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