JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 48-53.doi: 10.6040/j.issn.1671-7554.0.2014.560

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Effects of PADI4 silence on ovarian cancer cell line OVCAR3

ZHOU Jing1,2, CHANG Xiaotian2, ZHOU Ting2, CUI Yingying2, ZHANG Bei2, RONG Fengnian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Gynecology and Obstetrics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-10 Revised:2015-03-06 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To investigate the effects of PADI4 silence on ovarian cancer cell line OVCAR3. Methods OVCAR3 cells were transfected with siRNA of PADI4-siRNA group and negative control group in vitro respectively. The expressions of PADI4, p53 and p21 were detected with real-time PCR and Western blotting. Cell apoptosis, invasion and migration were measured with AnnexinV-PE/7-AAD assay and Transwell assay. Results Compared with that of the negative control group, PADI4 expression of OVCAR3 cells in PADI4-siRNA group decreased significantly, the invasion ability and apoptotic ratio also reduced (Pa<0.05, Pi<0.01). However, the chemotaxis ability showed no change. Compared with that of the negative control group, expression of p53 decreased, while p21 expression remained unchanged. Conclusion PADI4 silence altered the apoptosis and invasion abilities of ovarian cancer cell OVCAR3, demonstrating that PADI4 plays an important role in the control of malignant biological behavior of ovarian cancer.

Key words: Invasion, Apoptosis, Migration, Ovarian tumor, Peptidylarginine deiminase 4

CLC Number: 

  • R711.75
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