Table of Content

  

10 June 2015
Volume 53 Issue 6

IL-22 protects mice from acute severe pancreatitis via STAT3 signaling pathway

BAI Jinxia, BAI Jinyun, SHI Xiuju, XU Hongwei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  1-6.  doi:10.6040/j.issn.1671-7554.0.2015.001

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Objective To investigate the effect of interleukin-22 (IL-22) in acute severe pancreatitis (SAP) induced by L-arginine and its signal pathway. Methods Sixty male Balb/c mice were randomly divided into 4 groups, the normal control group(NaCl group, n=10), SAP group(n=30), treatment control group (PBS group, n=10) and treatment group(rIL-22 group, n=10). Mice were induced intraperitoneally with L-arginine(two doses, 4 g/kg each, 1 hour apart). PBS or rIL-22 (200 ng/time, 5 times) was administrated to mice at the indicated times in PBS and rIL-22 groups. Serum amylase and pancreas tissue histopathology were examined. IL-22RA1, regenerating islet-dedrived protein 3β(Reg3β), Reg3γ, B cell lymphoma/lewmia-2(Bcl-2), Bcl-xL mRNA were detected by Real-time PCR. STAT3 expression and activation were assessed by Western blotting. Mortality ratios in PBS and IL-22 group were counted. Results Seventy-two hours after L-arginine administration, typically histopathological changes were observed in SAP group. The expressions of Reg3β, Reg3γ, Bcl-2 and Bcl-xL mRNA decreased progressively, while serum amylaseand IL-22RA1 mRNA increased until 48 hours, but decreased apparently at 72 hours. Compared with those in PBS group, rIL-22 group showed lower serum amylase(P<0.05), slighter pathological changes and lower mortality(P<0.05). Compared with those in PBS group, the expressions of Reg3β, Reg3γ, Bcl-2, Bcl-xL, IL-22RA1 mRNA and phospho-STAT3 protein in rIL-22 group markedly increased(P<0.05). Conclusion Exogenous recombinant IL-22 protects mice from L-arginine induced SAP by enhancing the expressions of antimicrobial peptides and antiapoptotic genes through the STAT3 signaling pathway.

Knockout Grp78 expression increases the sensitivity of hepatoma cells to erlotinib

SONG Jia, LI Xin, LI Dan, YE Liping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  7-12.  doi:10.6040/j.issn.1671-7554.0.2014.811

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Objective To explore the effects of knockout Grp78 expression on the sensitivity of SMMC-7721 cells to erlotinib and the molecular mechanism. Methods The Grp78 expression in SMMC-7721 cells was downregulated with siRNA technique and the expression was identified with Western blotting. The survival rates of cells treated with different concentrations of erlotinib (0, 2.5, 5, 10, 20 μmol/L) were assessed with MTT method. Cell apoptosis was detected with flow cytometry. The morphological changes of apoptosis were evaluated with acridine orange staining. The expression of AKT, p-AKT, ERK and p-ERK were determined with Western blotting. Results The siRNA transfection significantly inhibited Grp78 expression after 24, 48 and 72 hours, and the strongest inhibitive effect appeared after 48 hours (P<0.05). Compared with SMMC-7721/siControl cells, erlotinib significantly inhibited the proliferation and promoted apoptosis of SMMC-7721/siRNA-Grp78 cells (P<0.05), and markedly reduced the expression of p-ERK and p-AKT in SMMC-7721/siRNA-Grp78 cells (P<0.05), while the total protein expression of ERK and AKT were not affected. Conclusion Knockout Grp78 expression increases the sensitivity of hepatoma cells to erlotinib by decreasing the phosphorylation of ERK and AKT.

Assessment of abdominal lymph node metastasis of VX2 hepatic cancer rabbits with percutaneous transhepatic lymphosonography

LI Xuefeng, WANG Shuanglong, YU Mei, CUI Hong, SUN Yuanyuan, DONG Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  13-17.  doi:10.6040/j.issn.1671-7554.0.2014.515

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Objective To investigate the applications of percutaneous transhepatic lymphosonography in assessing abdominal lymph node metastasis of VX2 hepatic cancer rabbits. Methods A total of 30 rabbits with VX2 tumor were divided into sham group (group A, n=5) and hepatic cancer group (group B, n=25). SonoVue was injected into the liver parenchyma inside and around the VX2 cancer to observe the normal heptic tissues, cancerous tissues and pericancerous tissues dynamically. The changes were quantatively analyzed. Results Of the 25 rabbits in group B, 11 (group B2) had lymph node metastasis while 14 (group B1) had not. Percutaneous transhepatic lymphosonography showed the liver tissues could be divided into 4 phases, interstitial space, defusing, plateau and dissipating phase. SonoVue disappeared in cancerous tissues faster than in pericancerous tissues, in which SonoVue sustained for 20 minutes. There were significant differences among normal heptic tissues, cancerous tissues and pericancerous tissues in plateau intensity(PTI)(P<0.01), and PTI in cancerous tissues was the greatest, followed by pericancerous tissues and normal heptic tissues. There were significant differences in pericancerous tissues of group A, B1 and B2, and PTI in group B2 was the greatest, followed by group B1 and group A (P<0.05). Conclusion Percutaneous transhepatic lymphosonography can provide detailed information of abdominal lymph node metastasis of hepatic cancer, which is useful for the choice of treatment and prediction of therapeutic effects.

Transportation and absorption of Alternaria mycotoxins in Caco-2 cells

ZHAO Kai, HAN Chunhui, LIN Xiaohui, JIANG Tao, YANG Dajin, SONG Jingdong, LI Fengqin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  18-22.  doi:10.6040/j.issn.1671-7554.0.2015.245

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Objective To develop and evaluate the Caco-2 cell model and to explore the transportation and absorption of 3 Alternaria mycotoxins. Methods Caco-2 cell model was established by inoculating Caco-2 cell on the polycarbonate membrane attached to the transwell plates and evaluated on the basis of transepithelium electrical resistance (TEER), activity of alkaline phosphatase (ALP), apparent permeability coefficient (P_app) of fluorescein and cytomorphology. Transportation and absorption of 3 Alternaria toxins including alternariol (AOH), alternariol methyl ether (AME) and tenuazonic acid (TeA) in different concentrations and at different time points were studied by determining the difference in toxin concentrations between apical side and basolateral side of the cell line. Results The TEER exceeded 300±15 Ω·cm² and ALP activity was statistically different (P<0.01) between apical side and basolateral side after 21 days' incubation. Clear microvilli and tight intercellular junctions were observed with transmission electron microscope. P_apps of AOH, AME and TeA varied between 1×10^-5cm/s and 10×10^-5cm/s. Transportation and absorptionof 3 Alternaria toxins at different concentrations ranged from 1.5% to 63.0%. Conclusion Intact and tight cell model was developed for Alternaria toxins transportation and absorption. AOH, AME and TeA were toxins easily or moderately absorbed by human beings.

The role of miR-485-5p in human colorectal cancer HCT116 cell invasion by regulating survivin expression

ZENG Renren, ZHANG Junhua, ZHANG Yinxu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  23-27.  doi:10.6040/j.issn.1671-7554.0.2015.083

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Objective To identify the role of miR-485-5p in human colorectal cancer HCT116 cell invasion. Methods HCT116 cells were cultured in vitro, and cellular morphology was observed with phase contrast microscope. The miR-485-5p was predicted with bioinformatics and identified with dual luciferase report system. Expressions of miR-485-5p and survivin were determined with Real-time PCR and Western blotting after transfection of miR-485-5p mimics. The invasion of HCT116 cells was detected in vitro with transwell chamber. Results Survivin expression was positive as detected by immunofluorescence cytochemistry. Dual luciferase reporter system indicated that miR-485-5p inhibited survivin expression by binding to 3'UTR of survivin mRNA. Real-time PCR and Western blotting showed that over-expression of miR-485-5p down-regulated survivin expression. The invasion of HCT116 cells was suppressed by over-expression of miR-485-5p. Conclusion The miR-485-5p may negatively regulate survivin expression by binding to its 3'UTR in human colorectal cancer HCT116 cells and inhibit cell invasion.

Impacts of Simvastatin on DNA methyltransferase of cell line NB4 in acute myeloid leukemia

YANG Juan, QIU Zongjian, SONG Qiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  28-32.  doi:10.6040/j.issn.1671-7554.0.2014.853

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Objective To explore the impacts of Simvastatin (SIM) on DNA methyltransferase (DNMT) of cell line NB4 in acute myeloid leukemia (AML) and to investigate the possible mechanism. Methods NB4 cells were treated with different concentrations of SIM (final concentration 0, 5, 10 and 15 μmol/L). DNMT activities were detected by DNMT activity/inhibition assay. The expressions of DNMT1, DNMT3A and P53 mRNA were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of DNMT1, DNMT3A and DNMT3B protein levels were analyzed by Western blotting. The apoptotic rate was measured by flow cytometry. Results The DNMT activity/inhibition assay showed a dose-dependent downregulation of DNMT activities after SIM treatment (P<0.05). The expression levels of DNMT1, DNMT3A mRNA and protein were downregulated while P53 mRNA were upregulated with the increase of SIM concentration (P<0.05). DNMT3B protein level did not change significantly (P>0.05). SIM induced NB4 cell apoptosis. Conclusion Simvastatin can downregulate the DNMT activities and expression levels in AML cell line NB4 and promote cell apopotosis, which may act as a potential DNA-hypomethylating agent for AML.

A microarray analysis of bortezomib-resistant gene expression in multiple myeloma

LIU Qiong, PU Yedi, DAI Guangxia, MA Jiale, YANG Jianxia, LI Lizhen, LI Hao, WANG Luqun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  33-38.  doi:10.6040/j.issn.1671-7554.0.2014.944

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Objective To investigate genes related to bortezomib-resistance phenotype by analyzing different gene expression patterns with cDNA microarray and explore the mechanism of bortezomib-resistant myeloma. Methods Gene expression profiles of KM3/BTZ and KM3 cell lines were compared using Affymetrix U133 plus 2.0 oligonucleotide microarray. Specific differently-expressed genes were chosen for further verification by real-time RT-PCR. Relevant data were analyzed with molecular annotation system MAS3.0 software and compared with documented resistance genes. Results It was found that the KM3/BTZ cell line had 670 differently expressed genes as compared to KM3, of which 32 genes were increased or decreased by more than 10 folds. These 32 genes were involved in the regulation of transcription and signal transduction. Some genes of ZNF and MS4A family and HSPB2 were lowly expressed in KM3, but highly expressed in KM3/BTZ. Except for JUN, 7 genes (CA12, CYP1B1, EPB41L3, HSPB2, MS4A4A, SDPR, PAWR) were further verified using real-time RT-PCR and results were consistent with the microarray. Conclusion Some members of ZNF and MS4A family and another 30 genes whose differential expression is more than 10 folds between KM3 and KM3/BTZ may be related to bortezomib-resistance in multiple myeloma cells. Combination of filteringgenes and detailed analysis of documented resistance genes provides a potential approach to investigate the mechanism of drug resistance in multiple myeloma cells.

Neuroprotective effects of histone deacetylases inhibitor 11r on rats following status epilepticus

QIAO Shan, HAN Tao, LI Wenna, WANG Shengjun, ZHAO Xiuhe, YANG Xue, LIU Xuewu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  39-43.  doi:10.6040/j.issn.1671-7554.0.2014.812

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Objective To investigate the neuroprotective effects of histone deacetylases(HDACs) inhibitor 11r on rats after seizures. Methods A total of 40 rats were randomly divided into 5 groups: control group,pilocarpine group, treatment group I(administered with 11r, 2.5 mg/kg, once a day,intraperitoneally in three consecutive days), treatment group Ⅱ(administered with 11r, 25 mg/kg, once a day, intraperitoneally in three consecutive days), and 11r pretreatment group. Lithium chloride and pilocarpine were used to induce status epilepticus. The behaviors of rats in each group were observed. HE staining was performed to detect neuronal degeneration in hippocampus 24 hours after pilocarpine-induced seizure. At 72 hour after seizures, Nissl staining and immunohistochemical staining were used to evaluate the loss of neurons and histone acetylation levels of hippocampal CA1 and CA3 regions in each group. Results Compared with pilocarpine group, 11r pretreatment group showed delayed pilocarpine-induced seizures and reduced mortality (P<0.05). Compared with those in pilocarpine group, the degrees of neuronal loss and degeneration in both treatment group Ⅰ and Ⅱ reduced (P<0.05), and the levels of histone acetylation in hippocampal CA1 and CA3 regions increased (P<0.05),while, the difference was not statistically significant between treatment group Ⅰ and Ⅱ (P>0.05). Conclusion New HDACs inhibitor 11r can improve pathological changes and histone acetylation levels of the brain in rats in the acute phase of pilocarpine-induced status epilepticus, which exerts neuroprotective effects on rats after seizures.

MRP8 regulates expression of IL-1β in gout cell model by NF-κB signaling pathway

ZHAO Lu, SUN Junbo, WEI Guimei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  44-47.  doi:10.6040/j.issn.1671-7554.0.2014.490

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Objective To explore the effect and underlying mechanism of myeloid-related protein 8 (MRP8) on gout. Methods Gout model in macrophages was induced by monosodium urate (MSU), and the expression of MRP8 in these model cells was detected by ELISA. Then, MRP8 siRNA was transferred into macrophage, and the effectiveness was verified by Western blotting. The influences of silenced MRP8 on the secretion of interleukin-1β (IL-1β), nuclear transcription factor-κB (NF-κB) and inhibitor of nuclear transcription factor-κB (I-κB) expressions in macrophages were detected by ELISA and Western blotting. Results The expression of MRP8 in macrophages increased after MSU induction (P<0.01) and MPR siRNA could silence MPR8 expression effectively. After the silence of MPR8, the IL-1β secretion decreased (P<0.05), NF-κB expression was down-regulated, while I-κB espression was up-regulated in macrophages. Conclusion MRP-8 participates in the process of IL-1β release in MSU-induced macrophages gout model, which may be regulated by NF-κB signal pathway.

Effects of PADI4 silence on ovarian cancer cell line OVCAR3

ZHOU Jing, CHANG Xiaotian, ZHOU Ting, CUI Yingying, ZHANG Bei, RONG Fengnian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  48-53.  doi:10.6040/j.issn.1671-7554.0.2014.560

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Objective To investigate the effects of PADI4 silence on ovarian cancer cell line OVCAR3. Methods OVCAR3 cells were transfected with siRNA of PADI4-siRNA group and negative control group in vitro respectively. The expressions of PADI4, p53 and p21 were detected with real-time PCR and Western blotting. Cell apoptosis, invasion and migration were measured with AnnexinV-PE/7-AAD assay and Transwell assay. Results Compared with that of the negative control group, PADI4 expression of OVCAR3 cells in PADI4-siRNA group decreased significantly, the invasion ability and apoptotic ratio also reduced (P_a<0.05, P_i<0.01). However, the chemotaxis ability showed no change. Compared with that of the negative control group, expression of p53 decreased, while p21 expression remained unchanged. Conclusion PADI4 silence altered the apoptosis and invasion abilities of ovarian cancer cell OVCAR3, demonstrating that PADI4 plays an important role in the control of malignant biological behavior of ovarian cancer.

Application of Onodera prognostic nutrition index in elderly patients with esophageal carcinoma treated with radiotherapy

YANG Shengsi, CHENG Yufeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  54-57.  doi:10.6040/j.issn.1671-7554.0.2014.718

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Objective To explore the applicative value of Onodera prognostic nutrition index (Onodera index) in elderly patients with esophageal carcinoma treated with radiotherapy. Methods Onodera index of 93 elderly patients with esophageal squamous-cell carcinoma treated with only radiotherapy were collected, and the patients were divided into good-nourishment group (Onodera index ≥45) and poor-nourishment group (Onodera index <45). Correlations between Onodera index and clinicopathological features, quality of life, radiation esophagitis and tumor regression were analyzed. Results Onodera index was found to be related to tumor size (P=0.002) and distant metastases (P=0.024). Good-nourishment group had significantly better quality of life especially therapeutic attitude (P=0.036), food intake (P=0.043) and self-care ability (P=0.028) than poor-nourishment group. Patients in poor-nourishment group had significantly more serious radiation esophagitis than those in good-nourishment group (P=0.032), while there was no significant difference in tumor regression between the two groups (P>0.05). Conclusion Onodera index is a valuable clinical indicator to estimate the nutritional status, clinicopathological features and early complications of elderly esophageal carcinoma patients treated with radiotherapy.

Analysis of esophageal motility characteristics and causes of non-obstructive dysphagia

DU Quanlin, CHU Chuanlian, LI Yanqing, CHEN Feixue, TIAN Baoling

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  58-62.  doi:10.6040/j.issn.1671-7554.0.2014.583

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Objective To analyze the causes and esophageal motility characteristics of patients with non-obstructive dysphagia (NOD) by esophageal high resolution manometry (HRM). Methods Esophageal HRM data of patients with dysphagia treated during Jan. 2011 and May 2014 at Qilu Hospital of Shandong University were collected. Patients suffering from obstructive dysphagia were excluded through history inquiry, endoscopic images and barium examination. Parameters including upper esophageal sphincter pressure (UESP), lower esophageal sphincter pressure (LESP), loweresophageal sphincter length (LESL), residual pressure, integrated relaxation pressure (IRP), distal contractile integral (DCI) and endoscopic results were analyzed. Results Among the 72 patients with NOD, the percentage of achalasia, functional dysphagia and gastroesophageal reflux disease (GERD) was 52.8% (38/72), 33.3% (24/72), 13.9% (10/720), respectively. Of the 38 patients with achalasia, the percentage of absent peristalsis, synchronous contraction, lower esophageal sphincter(LES) incomplete relaxation was 78.9%, 81.6% and 97.4%, respectively. Of the 24 patients with functional dysphagia, the cases of LES incomplete relaxation, upper esophageal sphincter(UES) incomplete relaxation, absent peristalsis, low amplitude peristalsis, distal and esophageal spasm was 16(66.7%), 7(29.2%), 4(16.7%), 8(33.3%) and 2(8.3%). The median (IQR) of LESP, residual pressure, IRP in achalasia patients were 17 (8, 28), 11 (6, 19), 9 (4, 18) respectively, which were higher than those of functional dysphagia group[9(5, 14), 4(0, 7), 2(0, 6), P<0.05]. No difference was found in UESP, LESL and DCI between the two groups. Residual pressure, IRP of patients with achalasia was higher than that of GERD group[11(6, 19) vs 4(3, 7), P=0.005, 9(4, 18) vs 3(1, 5), P=0.007]. There was no difference between functional dysphagia and GERD. Esophageal dilatation, food retention, and cardia stricture were the main endoscopic presentations of achalasia. Functional dysphagia group had no specific endoscopic change. In group of GERD, 3 cases showed erosive esophagitis and 7 cases had no obvious endoscopic abnormalities. Conclusions Achalasia is the most common cause of NOD, followed by functional dysphagia. The pathogenesis of NOD is diverse. Abnormal peristalsis and LES incomplete relaxation are the most two important factors of NOD. HRM is an important method for the diagnosis of NOD.

Correlation between helicobacter pylori infection and HIF-1α expression in patients with gastric polyps

FEI Zhiqiang, GONG Qi, ZHANG Hua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  63-67.  doi:10.6040/j.issn.1671-7554.0.2014.867

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Objective To investigate the correlation between helicobacter pylori (Hp) infection and HIF-1α expression in patients with gastric polyps. Methods A total of 134 cases of gastric polyps and 73 cases of gastric cancer were randomly selected as the experimental groups, and 117 cases of chronic gastritis served as the control group. The Hp infection in different parts of gastric polyps was detected with improved Giemsa staining; the protein expression of HIF- 1α was examined with immunohistochemistry; HIF-1α expression at both mRNA and protein levels were assessed with RT-PCR and Western blotting. The status of Hp infection and the levels of HIF-1α expression were statistically analyzed. Results Giemsa staining showed that the highest Hp infection rate appeared in chronic gastritis (80.34%), followed by gastric polyps (80.00%). Immunohistochemical examination displayed that the highest rate of positive HIF-1α expression occurred in gastric cancer and adenomatous polyp groups, accounting for 91.78% (67 cases) and 80.00% (4 cases), respectively. RT-PCR showed that the highest HIF-1α mRNA content was in stomach mucosa tissue of cancer, followed by that in adenomatous polyp. Western blotting showed that expression level of HIF-1α in gastric mucosa tissue of cancer was the highest, followed by that in adenomatous polyp, with statistically significant difference between the two groups (P<0.01). Conclusion Gastric polyps may associate with Hp infection and the HIF-1α protein expression of patients with gastric polyp is positive. However, the relationship between Hp infection and HIF-1α expression that influences the pathogenesis of gastric polyps remains to be further studied.

Expression and clinical significance of NPRL2 and Survivin in hepatocellular carcinoma

GU Xu, WANG Xiaqing, REN Wanhua, QIN Chengyong, HAN Guoqing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  68-72.  doi:10.6040/j.issn.1671-7554.0.2014.617

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Objective To investigate the expressions of NPRL2 and Survivin, and their association with the clinicopathologic parameters of hepatocellular carcinoma (HCC). Methods A total of 45 HCC tissues, paracancerous liver tissues and 15 normal liver tissues were collected. Expressions of NPRL2 and Survivin were determined with immunohistochemistry and RT-PCR methods. Results Compared with paracancerous liver tissues and normal liver tissues, HCC tissues had higher Survivin expression but lower NPRL2 expression (P<0.01), but no significant differences were found between paracancerous liver tissues and normal liver tissues (P>0.05). NPRL2 expression was significantly related to TNM stage (P<0.05). Survivin expression was associated with serum AFP concentration and TNM stage (P<0.05). By spearman relative analysis, there was significant correlation between NPRL2 mRNA and Survivin mRNA (r=-0.45, P<0.01). Conclusion In hepatocellular carcinoma, anti-oncogene NPRL2 was negatively related to oncogene Survivin. The two genes may play important roles in the occurrence and development of hepatocellular carcinoma.

Significance of different biomarkers on assessing the surgical treatment of necrotizing enterocolitis in the newborn

GAO Nannan, CHEN Dong, YU Yonghui, ZHANG Lili

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  73-76.  doi:10.6040/j.issn.1671-7554.0.2014.471

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Objective To investigate the significance of different biomarkers in evaluating the necessity and the optimal time of operation for neonatal necrotizing enterocolitis (NEC). Methods A nest case control study was conducted, in which 68 neonates with NEC (NEC group) were divided into two groups, non-surgical group (n=33) and surgical group (n=35), another 42 healthy neonates without infectious disease were enrolled as control group. From the start of hospitalization, to surgery or death as the end point, 9 variables including procalcitonin (PCT), C-reactive protein (CRP), platelet (PLT), lactic acid (Lac), fibrinogen (Fib), albumin, white blood cell (WBC), pH and blood sugar were examined. Case control, univariate analysis, and logistic regression analysis were performed. OR and 95%CIwere calculated. Results Of the 68 patients with NEC, 35 (51.47%) required surgical treatment for digestive tract perforation (n=29), septic shock (n=5), and hepatic portal venous gas (n=1). The mortality was 17.65% (n=12). Case control study showed that PCT, CRP, platelet, lactic acid, albumin, WBC, pH and blood sugar of the NEC group were significantly different from those of the control group (P<0.05). Univariate analysis indicated that PCT, CRP, platelet, lactic acid and albumin were associated with surgical therapy (P<0.05). Logistic regression analysis showed that platelet and PCT were significantly relevant to surgical therapy (P<0.05). ROC curve showed the cut-off value of PCT was 3.32 ng/mL, sensitivity 71.40%, specificity 81.80%, positive predictive value 80.60% and negative predictive value 72.90%. Conclusion Serial changes in biomarkers may predict progression of NEC to surgery or death in neonates. Persistently low platelet count and significantly increased PCT value (≥3.32 ng/mL) indicated that the neonates with NEC required surgical treatment.

Expression and clinical significance of miR-210 in the serum of patients with colorectal cancer

FANG Qian, QU Ailin, ZHANG Xin, DU Lutao, YANG Yongmei, WANG Chuanxin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  77-81.  doi:10.6040/j.issn.1671-7554.0.2015.060

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Objective To explore the expression and clinical significance of miR-210 in serum of patients with colorectal cancer(CRC). Methods The expression levels of miR-210 in serum of 48 CRC patients(CRC group) and 40 healthy people(control group) were tested by quantitative real-time PCR (qRT-PCR), followed by the analysis on the association of miR-210 expression levels with clinicopathological characteristics. The expression levels of miR-210 in serum of 12 patients in CRC group were determined before operation, at one month after operation and recurrence after operation. The expression levels of CA199 and CEA of the two groups were tested. The diagnostic value for CRC was evaluated by comparing the ROC curves of miR-210, CA199 and CEA. Results The expression level of serum miR-210 in CRC group was higher than that in control group, and the expression level of miR-210 in CRC group was associated with tumor size, local invasion, lymph nodes metastasis and clinical TNM stage (all P < 0.05). However, there was no significant correlation between miR-210 expression and gender, age, tumor type and differentiation(all P > 0.05). The expression levels of miR-210 in serum of 12 patients in CRC group at the first month after operation was lower than those before operation(P < 0.001), and its expressions in postoperative recurrence patients of CRC group were higher than that at the first month after operation(P < 0.001). ROC curve analysis showed that AUC for miR-210 was 0.835, which was higher than that of CEA(AUC: 0.672, Z=2.230, P < 0.05) and CA199(AUC: 0.651, Z=2.427, P < 0.05), while the diagnostic specificity, sensitivity, cutoff value, positive predictive value, negative predictive value and accuracy rate of miR-210 were 82.5%, 70.8%, 38.31, 82.9%, 70.2% and 76.1%, respectively. Conclusion Circulating miR-210 might be used as a novel non-invasive biomarker for early diagnosis of CRC.

Effect of goal-directed fluid therapy on abdominal surgery in elderly patients

PAN Guozheng, YOU Faping, WANG Shuai, BU Qing'ao, YUAN Qingzhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  82-85.  doi:10.6040/j.issn.1671-7554.0.2014.542

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Objective To investigate the effect of goal-directed fluid therapy on elderly patients undergoing abdominal surgery. Methods A total of 44 elderly patients who received abdominal surgery in our hospital during May 2013 and Nov 2014 were divided into two groups: goal-directed liquid treatment group (group A) and conventional treatment group (group B). The patients' clinical data were analyzed, including volume of fluid input, volume of urine per minute, intraoperative blood loss, hematocrit (HCT), creatinine (CR), blood urea nitrogen (BUN) on the first day after operation, exhaust time, hospitalization time, and complications. Results The volume of fluid input, volume of urine per minute, exhaust time, and hospitalization time for group A were significantly less/shorter than those for group B. There were no statistical differences in the other indexes between the two groups. Conclusion Goal-directed fluid therapy based on stroke volume variation (SVV) was safer and more reliable than traditional liquid treatment for elderly patients, because the volume of fluid input was more reasonable, and bowel functions recovered sooner.

Diagnostic value of pulmonary perfusion SPECT/CT for lower-limb deep-vein thrombosis cases of patient with suspected pulmonary embolism which had different Wells scores

WU Dayong, ZHANG Wenyan, FANG Baoshuan, QIU Gang, BIAN Yanzhu, HU Yujing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  86-89.  doi:10.6040/j.issn.1671-7554.0.2014.872

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Objective To explore the diagnostic value of pulmonary perfusion SPECT/CT for patients with lower-limb deep-vein thrombosis (DVT) who may have pulmonary embolism (PE) with different Wells scores. Methods The study included DVT patients (n=210) with suspected PE. According to the Wells scores recommended by the 2008 European Pulmonary Embolism Diagnosis Guideline, the patients were divided into the high risk group (score ≥ 7, n=89), moderate risk group (2≤ score <7, n=64) and low risk group (score < 2, n=57). The patients' pulmonary perfusion SPECT/CT images were retrospectively analyzed. The positive rates of the three groups were calculated. The data were analyzed with chi-square(χ²)test. Results The positive rate of the high, moderate and low risk group was 70.8% (63/89), 29.7% (19/64) and 10.5% (6/57), respectively. There were statistical differences among the groups (χ² = 57.47, P<0.05). Conclusion When DVT patients suspected of PE are diagnosed to have high risk or moderate risk by Wells scores, pulmonary perfusion SPECT/CT imaging should be recommended to confirm whether pulmonary embolism exists.

Use of metal block in total knee arthroplasty with tibial bone defect

HUANG Chuanwang, Salim Jeddo, ZHANG Yuankai, LI Deqiang, LIU Peilai, LI Ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  90-93.  doi:10.6040/j.issn.1671-7554.0.2014.786

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Objective To investigate the application of metal block in total knee arthroplasty with bone defect of tibia. Methods Data of 7 cases undergoing primary total knee arthroplasty with tibial plateau bone defect during Jan. 2012 and Dec. 2013 were retrospectively reviewed. The patients included 2 males and 5 females, aged 49-84 years, average 57 years. Postoperative complications in lower limbs were observed. After the follow-up of 1 year, the axial alignment of the lower extremity correction, presence of prosthesis loosening, displacement, collapse and so on were examined with X-ray and compared with preoperative and postoperative knee joint HSS scores. Results During the follow-up of 1 year, no complications such as infection or deep thrombosis in the lower limbs occurred. Knee pain disappeared, and axial alignment of the lower extremity was well corrected. The final X-ray examination showed no prosthesis loosening, displacement, collapse and so on. Preoperative HSS scored 17 to 29, average 23. Postoperative HSS scored 68 to 95, average 81. Conclusion Use of metal block to deal with tibial plateau bone defect for primary total knee arthroplasty can achieve excellent results without increasing complications.

Significance of type Ⅱ collagen biomarker C2C in the diagnosis of knee osteoarthritis

XIN Linwei, WANG Liming, LI Chaoxu, TANG Jicun, JIANG Mengyao, QU Quanli

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(6):  94-96.  doi:10.6040/j.issn.1671-7554.0.2014.454

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Objective To discuss the association between the level of type Ⅱ collagen biomarker C2C in urine and severity of knee osteoarthritis (KOA). Methods Urine samples and anteroposterior and lateral films of knee joint of 90 KOA patients (KOA group) and 20 healthy controls (control group) were collected and compared. According to Kellgren-Lawrence radiographic grading criteria, patients in the KOA group were divided into grade Ⅰ, Ⅱ, Ⅲ, and Ⅳ subgroups. Urine C2C level was detected with enzyme-linked immunosorbent assay (ELISA). Results Urine C2C level in KOA group was significantly higher than that in control group (P<0.01). In the subgroups, the C2C level in subgroup Ⅳ was the highest, followed by subgroup Ⅲ, Ⅱ and Ⅰ (P=0.000). The C2C level in subgroup I was similar to that in control group, with no significant difference (P>0.05). Conclusion The urine C2C level may serve as an indicator for the progression and curative effect of KOA, rather than an index for the early diagnosis of KOA.