Table of Content

  

10 July 2015
Volume 53 Issue 7

Mechanism of HGF/PARP-1 signaling pathway regulating invasion and metastasis of ovarian cancer

LÜ Shuqing, WEI Wei, ZHANG Cancan, TIAN Yongjie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  1-7.  doi:10.6040/j.issn.1671-7554.0.2015.171

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Objective To investigate the effects and signaling pathway of HGF-regulated PARP-1 expression on invasion and metastasis of ovarian cancer. Methods SKOV-3 cells were incubated with various concentrations of HGF to examine their invasive abilities by Transwell assay. Relative expression of PARP-1 after HGF treatment in SKOV-3 cells was analyzed by RT-PCR and Western blotting. SKOV3 cells were transfected with either negative control siRNA (NC-siRNA group) or PARP-1 siRNA (PARP1-siRNA group), and divided into different groups depending on different factors: blank control group; HGF group; PARP-siRNA group; HGF+PARP-siRNA group; NC-siRNA group; HGF+NC-siRNA group. The expression of PARP-1 in different groups was measured with Western blotting. The invasive abilities were examined with Transwell assay. The expression of MMP-2 was detected with ELISA. Results HGF promoted cell invasion in a concentration- and time-dependent manner in the SKOV-3 cell line. The expression of PARP-1 in SKOV-3 cells increased after HGF 40 ng/mL treatment for 24 hours. The expression of PARP-1 in PARP1-siRNA group was lower than that in NC-siRNA group (P<0.05). The transfection significantly reduced impacts of HGF on SKOV-3 cell invasion and the expression of MMP-2. Conclusion HGF promotes the invasion and metastasis of ovarian cancer. This effect may be related to the increased expression of MMP-2 which is mediated by PARP-1.

Therapeutic effects of human osteoblast-stimulating factor on osteoporosis of ovariectomized rats

FENG Xiaoyu, ZHANG Hongmei, CHE Xuanqiang, KANG Donghong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  8-12.  doi:10.6040/j.issn.1671-7554.0.2015.375

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Objective To investigate the therapeutic effects of human osteoblast-stimulating factor (OSF-1) on the osteoporosis of ovariectomized rats. Methods A total of 12 female rats aged 4 months were randomly divided into 4 groups: SHAM group, OVX group, human parathyroid hormone group (PTH group) and OSF-1 group. A small piece of adipose tissue was cut off from rats in SHAM group; bilateral ovariectomy was conducted in rats in the other groups. All rats were intervened per day separately since 10 weeks after the ovariectomy: SHAM and OVX groups received subcutaneous injection of 0.2 mL/d sodium chloride solution, PTH group was given subcutaneous injection of 20 μg/(kg·d) PTH, and OSF-1 group was injected subcutaneously with 80 μg/(kg·d) OSF-1. Pharmacological agents were administrated consecutively for 11 weeks, then all rats were sacrificed. Uterus was removed and weighed. Femur was removed and cleaned of adhering muscles and connective tissues. Microcomputed tomography (micro-CT) was used to scan proximal femur metaphysis. Results Uterus weight and bone mass in OVX group were significantly lower than those in SHAM group (P<0.01), and the microstructure of bone trabecula was destructed in OVX group. Compared with OVX group, bone morphometric parameters in PTH and OSF-1 groups showed significant increase on bone mineral density(BMD), BV/TV, Tb.N(P<0.01), while Tb.Sp and TBPf were lower (P<0.01, P<0.05),and bone trabecula were more conspicuous in PTH and OSF-1 groups. The parameters of BMD, BV/TV and Tb.N in PTH group were higher than those in OSF-1 group (P<0.01), and TBPf was lower than that in OSF-1 group (P<0.01). Conclusion Subcutaneous injection of 80 μg/(kg·d) OSF-1 can increase bone mass and improve bone microstructure in ovariectomized rats.

Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

WANG Jing, GAO Guixin, WAN Yunyan, LIU Qinghua, YAO Zhouhong, LIN Dianjie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  13-18.  doi:10.6040/j.issn.1671-7554.0.2014.719

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Objective To investigate the influence of traditional Chinese medicine, Yiqihuoxue-Yangyinjiedu recipe, on transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in lung tissues of mice with radiation-induced lung injury, and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods Mice were randomly divided into control group (n=21), drug group (n=21), radiation group (n=21), and treatment group (radiation+drug, n=21). The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored. The expressions of TGF-β1 and TNF-α were analyzed by RT-qPCR and immunohistochemistry at 1, 24, 72 h and 2, 4, 8, 24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1, TNF-α mRNA and protein in radiation group were higher than those in control group and drug group (P<0.05). Except at 1 h and 24 h, expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group (P<0.05). Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α, and may play an important role in preventing and treating radiation-induced lung injury, which may be related to inhibit the expressions of TGF-β1 and TNF-α.

Antitumor effect of endostatin synergized with tumor special DC-T cellular therapy

LI Xingyu, LIANG Jing, LI Yan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  19-23.  doi:10.6040/j.issn.1671-7554.0.2014.977

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Objective To investigate the antitumor effect of endostatin combined with tumor special DC-T cellular therapy on lung cancer in Lewis mice. Methods C57BL/6 mice models were established. Tumor antigen special DC-T cells from spleen cells and mice bones were cultured in vitro. The tumor-bearing mice were randomly divided into three groups: A group (PBS control group), B group (DC-T group), and C group (DC-T+endostatin group). The body weight of mice and tumor volume were measured. The VEGF and HIF-1α expressions were determined with Western blotting. The proportions of MDSC, TAM (M1/M2), mDC and CD8⁺T in suspended tumor cells of tumor tissues were detected with FCM. Results Compared with that in A group, tumor growth in B group (P<0.05) and C group (P<0.01) were inhibited; VEGF expression decreased while HIF-1α expression increased in B group (P<0.05) and C group (P<0.01); the proportions of MDSC and TAM (M2 type) were reduced, while proportions of mDC, TAM (M1 type) and CD8⁺T cells were elevated in B group (P<0.05) and C group (P<0.01). Conclusion Tumor special DC-T cells combined with endostatin strongly reduce tumor growth, and efficiently reverse the immunosuppression of tumor microenvironment, exhibiting synergistic and much better antitumor effects than monotherapy strategy.

Effect of cordycepin on the expression of CenpB of myelosuppression mice induced by cyclophosphamide

DONG Jialiang, ZHANG Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  24-28.  doi:10.6040/j.issn.1671-7554.0.2014.861

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Objective To investigate the effect of cordycepin on myelosuppression mice caused by intraperitoneal injection of cyclophosphamide. Methods Thirty mice were divided into the control group (group A), myelosuppression induced by cyclophosphamide group (group B) and myelosuppression intervened by cordycepin group (group C), 10 in each group. Mice in group A were intraperitoneally injected of normal saline for 12 d with the dosage of 5 mL/kg; Mice in group B were injected of normal saline for 5d after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg; Mice in group C were injected of cordycepin for 5 d with the dosage of 25 mg/kg after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg. The numbers of cells in peripheral blood and nucleated cells in femur of each mouse were counted. The content and the expression level of CenpB in leukocytes were detected by Western blotting and RT-PCR. Results Compared with those in group A, the numbers of leukocytes, red blood cells, platelets and nucleated cells, and the content and the expression level of CenpB were significantly decresed (P<0.05). Compared with those in group B, all the indexes, except for the number of red blood cells, were significantly incresed (P<0.05). Conclusion Cordycepin has a certain therapeutic function to myelosuppression mice caused by cyclophosphamide.

Screening of host protein interacting with early protein GP23 of bacteriophage LSPA1 of Salmonella Paratyphi A

MAO Pujia, FENG Mengdie, HONG Yu, XU Zeyang, ZHAO Jihua, YANG Hongwen, SONG Wuzhan, WANG Jiai, RAO Xiancai, HUANG Fen, JING Shenrong, ZENG Weikun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  29-33.  doi:10.6040/j.issn.1671-7554.0.2014.903

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Objective To screen the host protein interacting with early protein GP23 of bacteriophage LSPA1 of Salmonella Paratyphi A (S.Paratyphi A) CMCC50973. Methods The genome of S.Paratyphi A (CMCC50973) was digested by Sau3AⅠand the recovered bands (250 to 1 500 bp) were ligated with pAT plasmid to create the S.Paratyphi A genomic library. gp23, an early gene of phage LSPA1 genome, was ligated with pBT as a bait plasmid (pBT-gp23). The S.Paratyphi A genomic library plasmids and pBT-gp23 were co-transformed into the host bacteria KS. Furthermore, the bacterial two-hybrid was used to screen blue single bacterium. Positive clones were tested by the activity of LacZ. Positive plasmids were extracted, sequenced and analyzed. Results S.Paratyphi A genomic library was successfullyconstructed and its capatity met the requirements of the experiment. Positive rate of the library was about 100%. The gene of positive clone was putative purine permease by sequencing and BLAST. Conclusion Host protein(putative purine permease) may interact with early protein GP23 of bacteriophage LSPA1 of S.Paratyphi A.

Diagnostic value of frozen section for breast intraductal papillary neoplasms

DAI Gaosai, MA Tingting, JIANG Liyu, HUO Qiang, KONG Xiaoli, JING Chuyu, YANG Qifeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  34-38.  doi:10.6040/j.issn.1671-7554.0.2014.933

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Objective To investigate the significance of intraoperative frozen section in the diagnosis of intraductal papillary neoplasms and influencing factors. Methods Clinical data of 560 patients with intraductal papillary neoplasms confirmed with postoperative paraffin pathology in the Department of Breast Surgery, Qilu Hospital of Shandong University during Jan. 2007 to Dec. 2013 were retrospectively analyzed. The results of intraoperative frozen sections and postoperative paraffin sections were compared and factors affecting diagnostic accuracy rate were studied. Results The overall accuracy rate of intraoperative frozen sections was 85.1%. The accuracy rate of intraductal papilloma and intraductal papillary carcinoma was 88.9% and 49.0%, respectively (P<0.05). Of all patients, 16 cases (3.1%) with higher and 1 case (0.2%) with lower pathological grade were observed. The accuracy rate with and without nipple discharge was 89.2% and 79.4%, respectively (P<0.05). Conclusion Intraoperative frozen section can yield high accuracy for the diagnosis of intraductal papillary neoplasms. The accuracy rate of intraductal papilloma is higher than that of intraductal papillary carcinoma, and the accuracy rate is higher in patients with nipple discharge than those without.

Expression of netrin-1 in invasive ductal breast cancer and its relationship with tumor metastasis

GAO Yongsheng, WANG Chunjian, CAI Shuping, SUN Jujie, ZHANG Dexian, SUN Yanlin, MU Dianbin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  39-42.  doi:10.6040/j.issn.1671-7554.0.2015.069

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Objective To investigate the expression of netrin-1 in invasive ductal breast cancer and its clinical significance. Methods The expression of netrin-1 in 46 cases of invasive ductal breast cancer was detected with immunohistochemical SP method. Results Of all 46 cases, 30 cases had positive and 16 cases had negative expression of netrin-1. The positive rate of netrin-1 expression was 78.6% (11/14) in the group of breast cancer with lymph node metastasis. The expression of netrin-1 was not related to the patients' age, tumor size, clinical stage, expression of ER, PR, Her-2 and KI-67 (P>0.05), but was closely related to tumor metastasis (P<0.05). Conclusion Netrin-1 is abnormally expressed in breast cancer. It probably plays an important role in tumorigenesis and metastasis of breast cancer, and may be used as a marker for the prediction of breast cancer metastasis.

Expression and significance of Th22 and Th17 cells in the peripheral blood of cervical cancer

TIAN Xinli, JIAO Jun, ZHANG Teng, MA Daoxin, CUI Baoxia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  43-47.  doi:10.6040/j.issn.1671-7554.0.2014.964

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Objective To determine the expressions of Th22 and Th17 cells in the peripheral blood of cervical cancer and their contribution in the occurrence and progression of cervical cancer. Methods Flow cytometry was used to determine the percentages of Th22 cells and Th17 cells in the peripheral blood of 34 cervical cancer (CC) patients, 21 cervical intraepithelial neoplasia (CIN) patients and 19 healthy controls. ELISA was adopted to examine the plasma level of interleukin-22(IL-22). Results The percentages of Th22 cells (all P<0.01) and Th17 cells (CIN:P<0.05; CC:P<0.01) in CIN and CC patients were significantly increased compared with those in controls. The percentage of Th22 cells in cervical cancer was higher than that in CIN patients (P<0.01). An positive correlation between Th22 cells and Th17 cells was found in CIN patients (r=0.41, P<0.05). Th22 cells showed a positive correlation with Th17 cells in CC patients (r=0.49, P<0.01). The level of IL-22 in plasma was significantly increased in CC patients compared with that in controls (P<0.05). Conclusion There is a high percentage of Th22 and Th17 cells in CC patients. Th22 and Th17 cells may jointly participate in the pathogenesis and progression of CC.

Occurrence of epithelia-mesenchymal transition in endometrial adenocarci-noma and the roles of miR200a/ZEB1 signaling pathway in this process

ZHANG Xiaohui, YAN Lei, QI Shasha, LU Zhenzhen, LI Mingjiang, ZHAO Xingbo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  48-52.  doi:10.6040/j.issn.1671-7554.0.2015.184

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Objective To investigate the occurrence of epithelia-mesenchymal transition (EMT) in endometrial carcinoma and examine the roles of miR200a/zinc finger E-box-binding protein 1(ZEB1) signaling pathway in this process. Methods Different groups of samples were collected, including paraffin sections of endometrial adenocarcinoma tissues (group A, n=22), endometrial tissues after resection of uterine myoma(group B, n=45), endometrial adenocarcinoma samples obtained during operation(group C, n=15) and healthy endometrial tissues(group D, n=34). Expressions of E-cadherin, Vimentin and ZEB1 in group A and group B were investigated with immunohistochemistry. Expressions of E-cadherin, Vimentin and ZEB1 and their mRNA in group C and group D were evaluated with Western blotting and Real-time RT-PCR. Expression of miR200a in group C and D was determined with Real-time RT-PCR. Results ① Compared with group A, group B had significantly decreased expression of E-cadherin, and markedly increased expressions of Vimentin and ZEB1(both P<0.05). ② Compared with group C, group D had decreased E-cadherin and its mRNA expressions but increased expressions of Vimentin and its mRNA, and ZEB1 and its mRNA (all P<0.05). ③ Compared with group C, group D exhibited remarkably elevated miR200a, which was positively correlated to E-cadherin's mRNA(r=0.63, P<0.01)as indicated by a linear regression analysis. Conclusions EMT occurs in endometrial adenocarcinoma. The miR200/ZEB1signaling pathway can inhibit the important molecular markers in EMT.

Clinical efficacy of BSD2000 deep hyperthermia combined with chemotherapy of TP regimen for advanced ovarian cancer

CUI Yong, ZHANG Rongxiang, WANG Fuli, WANG Guoying, FENG Jianlin, ZHANG Haixia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  53-57.  doi:10.6040/j.issn.1671-7554.0.2014.841

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Objective To observe the clinical efficacy of BSD2000 deep hyperthermia combined with chemotherapy of docetaxel plus cisplatin (TP) regimen in the treatment of advanced ovarian cancer. Methods A total of 39 patients with advanced ovarian cancer were randomly divided into two groups: treatment group (n=20) receiving BSD2000 deep hyperthermia plus chemotherapy of TP regimen, and control group (n=19) undergoing chemotherapy of TP regimen. Results For the treatment and control group, the objective response rate (RR) was 90.0% vs 62.2%, RR of ascites was 55.0% vs 21.1%, quality of life score was 70.0% vs 31.6%, with statistically significant differences between the two groups (all P<0.05). The one-year and two-year survival rates for the treatment and control group were 77.8% vs 66.7% and 55.5% vs 44.4%, with no statistical difference (both P>0.05). Conclusion BSD2000 deephyperthermia combined with chemotherapy of TP regimen can significantly improve objective response rate and quality of life for patients with advanced ovarian cancer, and toxicity can be well tolerated.

Changes of vaginal microenvironment in 20 women after NuvaRing contraceptive ring placement

YANG Shujuan, SU Shili, DONG Baihua

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  58-60.  doi:10.6040/j.issn.1671-7554.0.2015.186

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Objective To study the changes of vaginal microenvironment after NuvaRing contraceptive ring placement. Methods For 20 women who received vaginal contraceptive ring placement, vaginal discharge of different cycles were examined with five joint inspections of vaginitis and microscopy. Concentrations of IL-2 and IL-8 in vaginal lavage of all subjects were determined with radioimmunoassay (RIA). Results After the ring was placed for a cycle, the concentrations of IL-2 and IL-8 were risen (P<0.05). However, after the ring was placed for 3 to 6 cycles, the concentrations of IL-2 and IL-8 dropped and returned normal (P>0.05). Five joint inspections of vaginitis and microscopy showed that pH value, quantification of leukocytes, lactobacilli, bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), and trichomonal vaginitis (TV) of vaginal discharge had no significant difference compared with the indexes before the ring was placed. Conclusion Vaginal contraceptive ring increases the concentrations of IL-2 and IL-8 initially, which will return normal after vagina adaptes to the ring. Vaginal ring has no obvious effects on vaginal acid circumstances and couldn't lead to the growth of other bacteria.

Effect of Gonadotrophin on the in vitro maturation of immature oocytes from controlled ovarian stimulation

SONG Xinggui, CHEN Zijiang, MA Shuiying, WU Keliang, LI Mei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  61-64.  doi:10.6040/j.issn.1671-7554.0.2014.1017

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Objective To evaluate the effect of Follicle-stimulating hormone (FSH) and Human chrorionic gonadotrophin (HCG) on in vitro maturation of immature oocytes obtained from controlled ovarian stimulation (COH) cycles. Methods MⅠ and GV stage oocytes were collected from COH cycles for intracytoplasmic sperm injection (ICSI) treatment. All of these immature oocytes were divided into two groups (donadotrophin treatment group and non-donadotrophin treatment group) and cultured for 24 to 28 hours in insemination media. The donadotrophin group was treated with 0.075 IU/mL FSH and 0.15 IU/mL HCG. Matured oocytes were given ICSI. The rates of maturation, normal fertilization, cleavage, and high-quality embryo were recorded and compared. Results For MⅠ oocytes, there were no difference between the two groups in maturation rate, fertilization rate, cleavage rate and high-quality embryo rate (P>0.05). For GV oocytes, high-quality embryo rate in donadotrophin treatment group was higher than that in non-donadotrophin treatment group (31.3% vs 4.2%, P<0.05). Conclusion Immature oocytes from COH can mature in vitro whether hormones are added or not. During the maturation of the GV stage oocytes, donadotrophin can increasethe developmental potential of oocytes.

Reproductive outcomes of different laparoscopic operations for ectopic pregnancy

DONG Yanlei, ZHU Lin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  65-67.  doi:10.6040/j.issn.1671-7554.0.2015.173

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Objective To investigate the reproductive outcomes of laparoscopic salpingectomy and salpingotomy after ectopic pregnancy. Methods A total of 127 ectopic pregnant patients were involved during Jan. and Dec. 2012, who wished to preserve reproductive ability. The patients were divided into two groups according to different types of operations. Patients in group A (n=64) received salpingectomy, while patients in group B (n=63) underwent salpingotomy. The reproductive outcomes after 2 years of follow-up were investigated. Results The recurrent ectopic pregnancy rate was 4.7%(3/64) in group A and 15.9%(10/63) in group B, with significant difference (P<0.05). Intrauterine pregnancy rate was 45.3%(29/64) in group A and 49.2%(31/63) in group B, with no significant difference (P>0.05). Conclusion If patients with ectopic pregnancy have normal contralateral tubes, we suggest salpingectomy for them.

Clinical evaluation of adult patients with bronchiectasis

QI Qian, WANG Wen, LI Tao, LI Yu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  68-72.  doi:10.6040/j.issn.1671-7554.0.2015.066

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Objective To evaluate the clinical manifestations of adult patients with bronchiectasis, and to explore the difference among diverse imaging types on high-resolution chest CT scan. Methods Adult patients diagnosed with bronchiectasis by high-resolution chest CT scan were consecutively recruited from 2 general hospitals in Shandong Province from September 2011 to August 2014. Etiology, clinical presentations, radiographic features, pulmonary function and sputum microbiology were analyzed. The difference among diverse imaging types on high-resolution chest CT scan was determined. Results A total of 313 adult patients with bronchiectasis were included. Idiopathic bronchiectasis (217 cases, 69.3%) was the most common reason, followed by post-tuberculosis bronchiectasis (57 cases, 18.2%). The most vulnerable lobe was lower lobe of left lung (219 cases, 70.0%). The characteristic presentations were cough (296 cases, 94.6%), expectoration (285 cases, 91.1%), hemoptysis (127 cases, 40.6%), and moist rales on chest examination (195 cases, 62.3%). Pulmonary function abnormalities were identified in 218 patients (69.6%), in whom obstructive ventilatory dysfunction was the most common type (136 cases, 62.4%). One hundred and forty-four patients' sputum specimens were tested positive (144 cases, 46.0%). The most commonly isolated pathogen was Pseudomonas aeruginosa (106 cases, 73.6%). Patients with cystic bronchiectasis had a higher frequency of expectoration, dyspnea, fever and fatigue (all P<0.001). Conclusion A large proportion of bronchiectasis patients are idiopathic. The predominant clinical features are cough, expectoration, hemoptysis and fixed moist rales. Patients with cystic bronchiectasis are prone to suffer from more severe clinical manifestations.

A feasibility study for boost target delineation using ¹⁸F-FDG PET/CT in local advanced non-small cell lung cancer

GAO Ang, WANG Shijiang, FU Zheng, SUN Xindong, YU Jinming, MENG Xue

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  73-77.  doi:10.6040/j.issn.1671-7554.0.2015.049

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Objective To evaluate whether during-radiotherapy or post-radiotherapy ¹⁸F-FDG uptake locations within tumour can be identified by a pre-radiotherapy scan for non-small cell lung cancer, and to explore the optimal biological sub-volume of the primary tumor for dose escalation. Methods ¹⁸F-FDG PET/CT scans were performed at pre-radiotherapy, during-radiotherapy (40 Gy) or post-radiotherapy. The region of interests were auto-delineated using the 40%-70% maximal standardized uptake value (SUV_max) thresholds using pre-radiotherapy scan. Overlap fractions between pre-radiotherapy scan and during-radiotherapy or post-radiotherapy scan were calculated. Then, a Spearman correlation was used to analyze the correlations between the volumes of the region of interests and sensitivities of radiotherapy. Results The 50% SUV_max-delineated region of interests had large overlap fractions with the 40% SUV_max-delineated and manual-delineated regions of high uptake at during-radiotherapy, with the values being (74.3±15.9)% and (84.4±15.3)%, respectively. The overlap fractions of 50% SUV_max-delineated region of interests and the 80% SUV_max-delineated regions of high uptake at post-radiotherapy were more than 72%. The volume of 50% SUV_max-delineated region of interests was smaller than the gross tumor volume (GTV), with the value of being (29.4±12.3)%. However, the 50% SUV_max-delineated region of interests had no correlations with the sensitivities of radiotherapy. Conclusion A ¹⁸F-FDG PET/CT scan at pre-radiotherapy can identify the high ¹⁸F-FDG uptake regions at during-radiotherapy and post-radiotherapy. The 50% SUV_max-delineated volume may be a suitable region for dose boosting.

Dose-effect relationship for keloids between skin tension and radiotherapy

LI Zhonghua, WANG Haiqin, SUN Yukai, LU Changhui, YANG Bo, WANG Yimin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  78-81.  doi:10.6040/j.issn.1671-7554.0.2014.819

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Objective To evaluate the association of radiation dose for high-tension and low-tension keloids and to explore the optimal radiotherapy. Methods Of the 48 patients, 25 had two keloids in high-tension parts such as anterior chest wall, scapulae and pubis, and 23 had two keloids in low-tension parts such as ear and neck. The patients were divided into two groups: high radiation dose group (group A) and low radiation dose group (group B). All patients received fractional CO₂ laser to remove the hypertrophic scar. After wound healing, patients in group A were treated with 7 Gy 3 times by ³²P applicator; patients in group B were treated with 5 Gy 3 times. The clinical efficacy and side effects were compared between the two groups. Results For low-tension parts: there were no significant differences between the two groups in cure rates and VSS scores (95.2% vs 80.9%, 5.21±0.78 vs 5.43±0.53, χ²=2.043, t=1.069, P>0.05). The ratio of side effects in group A was significantly higher than that in group B (71.4% vs 38.1%, χ²=4.709, P<0.05). For high-tension parts: the cure rate and VSS in group A were markedly higher than those in group B (75.0% vs 41.7%, 5.56±0.51 vs 6.96±0.71, χ²=5.486, t=7.846, P<0.05). Conclusion For better curative effects, keloids in low-tension parts should be treated with 15 Gy in 3 fractions, while keloids in high-tension parts should be treated with 21 Gy in 3 fractions.

Expressions of PDCD4 and survivin in condyloma acuminatum, Bowenoid papulosis, Bowen disease and squamous cell carcinoma

YU Shuping, LI Xuefei, WANG Dan, WANG Yukun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  82-86.  doi:10.6040/j.issn.1671-7554.0.2014.864

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Objective To explore the expressions of programmed cell death 4 (PDCD4) and survivin in patients with condyloma acuminatum (CA), Bowenoid papulosis (BP), Bowen disease (BD) and squamous cell carcinoma (SCC). Methods The expressions of PDCD4 and survivin were detected in normal foreskin (n=10), CA (n=30), BP (n=28), BD (n=10) and SCC (n=11) with immunohistochemical techniques. Results The expression of PDCD4 in BP, BD and SCC was significantly lower than that in foreskin control (P<0.05), and it was lower in SCC than in CA (P<0.05). The expression of survivin in both BD and SCC was significantly higher than that in control (P<0.05), and it was higher in SCC than in CA (P<0.05). Conclusions PDCD4 can inhibit benign neoplasms from malignant transformation. The overexpression of survivin can inhibit cell apoptosis and promote proliferation.

Clinico-pathological characteristics and diagnostic biomarkers in patients with esophageal carcinoma accompanied by neuroendocrine cell differentiation

LIU Lianke, SHAO Mingwen, MA Lan, SUN Jing, GUAN Dan, SHU Yongqian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  87-91.  doi:10.6040/j.issn.1671-7554.0.2015.138

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Objective To investigate the clinico-pathological characteristics and diagnostic markers in esophageal carcinoma accompanied by neuroendocrine cell differentiation (E-NED). Methods The clinico-pathological data of 378 patients with poorly differentiated esophageal carcinoma from Jan. 2008 to Dec. 2013, including 349 without neuroendocrine differentiation (E-NNED) and 29 with neuroendocrine differentiation (E-NED), were retrospectively analyzed. The expressions of synaptophysin (SYN), chromogranin A (CgA), neuron-specific enolase (NSE), neural cell adhesion molecule (CD56), protein gene product (PGP9.5), secretagogin (SCGN) and thyroid transcription factor 1 (TTF-1) in the esophageal carcinoma tissues of 29 patients with E-NED and 20 ones randomly selected from the 349 patients with E-NNED were detected using immunohistochemistry. Results There were no significant differences between the patients with E-NED and E-NNED in terms of the age, gender, length of lesion and other clinico-pathological factors. Except for TTF-1, the expressions of Syn, CgA, NSE, CD56, PGP9.5 and SCGN were significantly higher in patients with E-NED than those with E-NNED (P<0.05). The sensitivity, positive predictive value anddiagnostic accuracy were all higher in the combined detection of Syn and CD56, PGP9.5 or SCGN than Syn and CgA (P<0.05). There were no significant differences between the patients with E-NED and E-NNED regarding 1, 2 and 3-year survival rates. Conclusion E-NED and E-NNED may be different pathological types of esophageal carcinoma. Both PGP9.5 and SCGN can be considered as the diagnostic markers of E-NED, and the combined detection of Syn and CD56, PGP9.5 or SCGN can improve the sensitivity and diagnostic accuracy.

Influence of meteorological factors on chronic bronchitis mortality of the susceptible population of chronic bronchitis in Pingjiang County, Hunan Province, China

WANG Ning, DING Guoyong, GAO Lu, LI Jie, LI Xuewen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(7):  92-96.  doi:10.6040/j.issn.1671-7554.0.2014.994

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Objective To investigate the relationship between chronic bronchitis mortality and meteorological factors in Pingjiang County, Hunan Province, China. Methods The death data of chronic bronchitis, demographic data and corresponding meteorological data from January 2009 to December 2011 in Pingjiang County were collected and analyzed using the Spearman correlation and principal component analysis. Results Chronic bronchitis mortality of population over the age of 45 was significantly increased and regarded as susceptible population. Chronic bronchitis mortality of male was higher than that of female in population over the age of 50. Chronic bronchitis mortality was higher in winter and spring, and had significant correlations with temperature, barometric pressure, vapor pressure, sunshine time and wind velocity. Conclusion Population over the age of 45 is regarded as susceptible one of chronic bronchitis in Pingjiang County. Chronic bronchitis mortality has significant differences in season and gender. Temperature, barometric pressure, vapor pressure, sunshine time and wind velocity are the main meteorological factors.