JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 24-28.doi: 10.6040/j.issn.1671-7554.0.2014.861

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Effect of cordycepin on the expression of CenpB of myelosuppression mice induced by cyclophosphamide

DONG Jialiang, ZHANG Lei   

  1. Department of Biochemistry, Basic Medical College of Liaoning Medical University, Jinzhou 121000, Liaoning, China
  • Received:2014-11-24 Revised:2015-03-25 Online:2015-07-10 Published:2015-07-10

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Abstract: Objective To investigate the effect of cordycepin on myelosuppression mice caused by intraperitoneal injection of cyclophosphamide. Methods Thirty mice were divided into the control group (group A), myelosuppression induced by cyclophosphamide group (group B) and myelosuppression intervened by cordycepin group (group C), 10 in each group. Mice in group A were intraperitoneally injected of normal saline for 12 d with the dosage of 5 mL/kg; Mice in group B were injected of normal saline for 5d after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg; Mice in group C were injected of cordycepin for 5 d with the dosage of 25 mg/kg after injection of cyclophosphamide for 7 d with the dosage of 50 mg/kg. The numbers of cells in peripheral blood and nucleated cells in femur of each mouse were counted. The content and the expression level of CenpB in leukocytes were detected by Western blotting and RT-PCR. Results Compared with those in group A, the numbers of leukocytes, red blood cells, platelets and nucleated cells, and the content and the expression level of CenpB were significantly decresed (P<0.05). Compared with those in group B, all the indexes, except for the number of red blood cells, were significantly incresed (P<0.05). Conclusion Cordycepin has a certain therapeutic function to myelosuppression mice caused by cyclophosphamide.

Key words: Myelosuppression, Cordycepin, CenpB protein, Cyclophosphamide

CLC Number: 

  • R965.1
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