JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 13-18.doi: 10.6040/j.issn.1671-7554.0.2014.719

Previous Articles     Next Articles

Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

WANG Jing1, GAO Guixin2, WAN Yunyan1, LIU Qinghua1, YAO Zhouhong1, LIN Dianjie1   

  1. 1. Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Oncology Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-10-17 Revised:2015-03-27 Online:2015-07-10 Published:2015-07-10

PDF (PC)

244

Abstract: Objective To investigate the influence of traditional Chinese medicine, Yiqihuoxue-Yangyinjiedu recipe, on transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in lung tissues of mice with radiation-induced lung injury, and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods Mice were randomly divided into control group (n=21), drug group (n=21), radiation group (n=21), and treatment group (radiation+drug, n=21). The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored. The expressions of TGF-β1 and TNF-α were analyzed by RT-qPCR and immunohistochemistry at 1, 24, 72 h and 2, 4, 8, 24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1, TNF-α mRNA and protein in radiation group were higher than those in control group and drug group (P<0.05). Except at 1 h and 24 h, expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group (P<0.05). Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α, and may play an important role in preventing and treating radiation-induced lung injury, which may be related to inhibit the expressions of TGF-β1 and TNF-α.

Key words: Radiation-induced lung injury, Tumor necrosis factor-α, Transforming growth factor-β1, Yiqihuoxue-Yangyinjiedu recipe

CLC Number: 

  • R563.1
[1] Mazeron R, Etienne-Mastroianni B, Pérol D, et al. Predictive factors of late radiation fibrosis: a prospective study in non-small cell lung cancer[J]. Int J Radiat Oncol Biol Phys, 2010, 77(1): 38-43.
[2] Oh YT, Noh OK, Jang H, et al. The features of radiation induced lung fibrosis related with dosimetric parameters[J]. Radiother Oncol, 2012, 102(3):343-346.
[3] Arkovitz MS, Garcia VF, Szabó C, et al. Decreased pulmonary compliance is an early indicator of pulmonary oxygen injury[J]. J Surg Res, 1997, 67(2): 193-198.
[4] Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale[J]. J Clin Pathol, 1988, 41(4): 467-470.
[5] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408.
[6] Friedrichs K, Gluba S, Eidtmann H, et al. Overexpression of p53 and prognosis in breast cancer[J]. Cancer, 1993, 72(12): 3641-3647.
[7] Hill RP, Zaidi A, Mahmood J, et al. Investigations into the role of inflammation in normal tissue response to irradiation[J]. Radiother Oncol, 2011, 101(1): 73-79.
[8] Kim JY, Kim YS, Kim YK, et al. The TGF-beta1 dynamics during radiation therapy and its correlation to symptomatic radiation pneumonitis in lung cancer patients[J]. Radiat Oncol, 2009, 4: 59.
[9] Sanderson N, Factor V, Nagy P, et al. Hepatic expression of mature transforming growth factor beta1 in transgenic mice results in multiple tissue lesions[J]. Proc Natl Acad Sci U S A, 1995, 92(7): 2572-2576.
[10] 黄莺, 叶燕青, 孙洁民.肺纤维化形成过程中大鼠肺成纤维细胞分泌TGF-βmRNA及HGF mRNA对肺泡II型上皮细胞的影响[J]. 重庆医学, 2010, 39(19): 2548-2549, 2552. HUANG Ying, YE Yanqing, SUN Jiemin. Effect of pulmonary fibroblast express TGF-β mRNA and HGF mRNA to II alveolar epithelial cell in the rat model of pingyangmycin induced pulmonary fibrosis[J]. Chongqing Medicine, 2010, 39(19): 2548-2549, 2552.
[11] Chung EJ, Hudak K, Horton JA, et al. Transforming growth factor alpha is a critical mediator of radiation lung injury[J]. Radiat Res. 2014, 182(3): 350-362.
[12] 高桂新, 王成祥.周平安教授治疗放射性肺炎的临床经验[J].北京中医药大学学报:中医临床版, 2009, 16(4): 24-25.
[13] Dong XR, Wang JN, Liu L, et al. Modulation of radiation-induced tumour necrosis factor-α and transforming growth factor β1 expression in the lung tissue by Shengqi Fuzheng injection[J]. Mol Med Rep, 2010, 3(4): 621-627.
[14] Zhang K, Yang S, Zhu Y, et al. Protection against acute radiation-induced lung injury: a novel role for the anti-angiogenic agent Endostar[J]. Mol Med Rep, 2012, 6(2): 309-315.
[15] Wang J, Xu HW, Li BS, et al. Preliminary study of protective effects of flavonoids against radiation-induced lung injury in mice[J]. Asian Pac J Cancer Prev, 2012, 13(12): 6441-6446.
[16] Xie CH, Zhang MS, Zhou YF, et al. Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-alpha and TGF-beta1 in mice[J]. Oncol Rep, 2006, 15(6): 1429-1436.
[17] 谢丛华, 周云峰, 彭纲, 等.当归对小鼠放射性肺损伤过程中TNF-α水平的影响[J].中华放射肿瘤学杂志, 2005, 14(1): 59-63. XIE Conghua, ZHOU Yunfeng, PENG Gang, et al. Modulation by Angelia Sinensis the expression of tumor necrosis factor αin radiation-induced damage of the lung[J]. Chin J Radiat Oncol, 2005, 14(1): 59-63.
[1] YOU Jiebing, SUN Zhongwen, DU Juan, XU Wenjuan, WANG Yalin, LI Shuai, ZHU Meijia. Effects of rosiglitazone and insulin on the expression of TIPE2 and cell apoptosis in the brain of type 2 diabetic GK rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 43-48.
[2] LIN Chen, GUO Fang, JI Weina, YU Wencheng. Levels of peripheral blood TNF-α, sTREM-1 and α1-APG in community acquired pneumonia patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 61-64.
[3] ZHANG Yingzhe, WU Dongjin, PENG Changliang, LI Bohan, SONG Yang, ZHANG Cheng, ZHAO Jie, LI Dequan, YANG Zhongyan, LIU Peng, ZHAO Kun, MA Shengzhong, GAO Chunzheng. Effect of silencing TGF-β1 expression by small interfering RNA on ossification of the ligamentum flavum in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 27-33.
[4] ZHANG Jin-hang1, ZHANG Lu-wei1, LIU Xin-nong2, TIAN Jun1. Expression of Tribble3 in db/db mice  renal tissues and the possible role in renal fibrosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(2): 38-43.
[5] SUN-Yue, LIU Xiang-lan, WANG-Qun, GUO-Chun, SHI Yong-yu, ZHANG Li-ning. Identification and application of specific antibody against human TIPE3， a new member of TIPE family [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(1): 5-9.
[6] HAN Xiaojuan1, MA Xue-qiang2, DU Yi-feng1. Effects of Piper Kadsura Ohwi extracts on β-amyloid protein-induced activation of microglia in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 6-10.
[7] LIU Na, CAI Ke-li. Effect of Angiotensin (1-7) and Perindopril on expression of TGF-β1 in diabetic retina [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 62-65.
[8] CHI Wei-wei1, GAO Hai-yan2, GAO Hai-dong3. The molecular mechanism of transforming growth factor-β1-induced transmembrane protein participating in the development of breast cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 26-29.
[9] XU Hui1, WANG Yi-guo2, LIU Chang-hong2, LIU Qian2, DU Juan3. Expression of HBV and its effects on the transdifferentiation in HK-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 15-20.
[10] HAN Yan-xin, CONG Ya-qin, WANG Zhi-min. Immuno-suppressive effects mediated by mesenchymal stem cells of
myelodysplastic syndrome patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 97-.
[11] WANG Yue-heng1， XING Yi2， WANG Jiu-ling1， DAI Jun3， LI Shu-ling1. Changes and significances of serum visfatin and TNF-α in
patients with polycystic ovary syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 94-.
[12] JIAO Qi1,2, WANG Wei3, FAN Quanxin4. Effect of different balanced ultrafiltration volumes on inflammatory  mediators during cardiopulmonary bypass [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 136-.
[13] SUN Yao, LIU Dong-mei, CHENG Yan, L Yong-heng. Effect of metformin on myocardial fibrosis in diabetic rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 83-85.
[14] TIAN Meirong1, MA Yuyan1, WANG Leiyi2, FAN YueLian1, LIU Zhenping2. Expression of interleukin-17 in placenta and peripheralblood of patients with preeclampsia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 105-.
[15] XU Hua1,HOU Weikai1,ZHANG Li2,ZHU Lei2,　LOU Fuchen1,ZHANG Ying1,WANG Qian1,YU Shan1. Significance of Apelin and TNF-α level changes in obese patients with type 2 diabetes mellitus before and after treatment [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(3): 24-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd