JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 13-18.doi: 10.6040/j.issn.1671-7554.0.2014.719

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Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

WANG Jing1, GAO Guixin2, WAN Yunyan1, LIU Qinghua1, YAO Zhouhong1, LIN Dianjie1   

  1. 1. Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Oncology Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-10-17 Revised:2015-03-27 Online:2015-07-10 Published:2015-07-10

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Abstract: Objective To investigate the influence of traditional Chinese medicine, Yiqihuoxue-Yangyinjiedu recipe, on transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in lung tissues of mice with radiation-induced lung injury, and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods Mice were randomly divided into control group (n=21), drug group (n=21), radiation group (n=21), and treatment group (radiation+drug, n=21). The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored. The expressions of TGF-β1 and TNF-α were analyzed by RT-qPCR and immunohistochemistry at 1, 24, 72 h and 2, 4, 8, 24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1, TNF-α mRNA and protein in radiation group were higher than those in control group and drug group (P<0.05). Except at 1 h and 24 h, expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group (P<0.05). Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α, and may play an important role in preventing and treating radiation-induced lung injury, which may be related to inhibit the expressions of TGF-β1 and TNF-α.

Key words: Radiation-induced lung injury, Tumor necrosis factor-α, Transforming growth factor-β1, Yiqihuoxue-Yangyinjiedu recipe

CLC Number: 

  • R563.1
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