JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 43-48.doi: 10.6040/j.issn.1671-7554.0.2014.927

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Effects of rosiglitazone and insulin on the expression of TIPE2 and cell apoptosis in the brain of type 2 diabetic GK rats

YOU Jiebing1, SUN Zhongwen2, DU Juan3, XU Wenjuan4, WANG Yalin1, LI Shuai1, ZHU Meijia5   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Neurology, Yuhuangding Hospital of Yantai City, Yantai 264000, Shandong, China;
    3. Medical Research Center, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    4. Department of Neurology, General Hospital of Jinan Military Area, Jinan 250031, China;
    5. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
  • Received:2014-12-09 Revised:2015-02-28 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To explore the effects of rosiglitazone and insulin on the expression of tumor necrosis factor-α induced protein 8 like-2 (TIPE2) and cell apoptosis in the brain of type 2 diabetic GK rats, and to investigate the mechanism involved. Methods A total of 25 rats were enrolled and divided into 5 groups, including 3-month-old Wistar rats in the 3Wistar group, 3-month-old GK rats in the 3GK group, 6-month-old GK rats in the 6GK group, rosiglitazone treated 6-month-old GK rats in the RSG+6GK group, and insulin treated 6-month-old GK rats in the INS+6GK group, with 5 rats in each group. Immunohistochemistry (IHC) was used to observe the quantity and features of TIPE2expression in different rats' brains. RT-PCR and Western blotting were adopted respectively to detect the mRNA and protein level of TIPE2 in different rats' brains. Immunofluorescence (IH) was applied to observe the numbers of apoptotic cells in different rats' brains. Results There were significant differences among the groups in the staining intensity, numbers of positively-stained cells and positively-stained vessels, level of mRNA and protein, and expression of TIPE2. The above indexes were highest in the 6GK group, followed by the 3GK group, 3Wistar group, RSG+6GK group and INS+6GK group (all P< 0.05). The number of apoptotic cells was positively associated with the expression of TIPE2 (r=0.9816, P< 0.05). Conclusion Type 2 diabetes can promote the expression of TIPE2 and cell apoptosis. With age and progression of diabetes mellitus, expression of TIPE2 and the number of apoptotic cells gradually increase. After rosiglitazone and insulin treatment, the expression of TIPE2 reduces and cell apoptosis is inhibited. In the course of diabetes mellitus, TIPE2 accelerates the cell apoptosis.

Key words: Tumor necrosis factor-&alpha, Diabetes mellitus, agonist, induced protein 8 like-2, Cell apoptosis, PPAR&gamma, Insulin

CLC Number: 

  • R741
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