JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 63-68.doi: 10.6040/j.issn.1671-7554.0.2016.1312

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Expression of microRNA-200b in granulosa cells of PCOS patients and its significance

JIA Yueyue, LIU Hongbin, LI Jingbo, LI Jing, ZHANG Jiangtao, SUN Mei, SHI Yuhua   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China
  • Received:2016-10-14 Online:2017-01-10 Published:2017-01-10

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Abstract: Objective To explore the expression and clinicopathological significance of microRNA-200b(miR-200b)in ovarian granulosa cells of patients suffering from polycystic ovary syndrome(PCOS)with insulin resistance. Methods A total of 120 PCOS patients(78 with insulin resistance and 42 without)and 72 controls were enrolled. The expression of miR-200b was examined in ovarian granulosa cells by real-time quantitative reverse transcription PCR(qRT-PCR), and the clinical significance was analyzed. Results PCOS patients with insulin resistance had higher morbidity of overweight, obesity, hypertensive disease, hyperglycemia, dyslipidemia and metabolic syndrome than PCOS patients without insulin resistance and controls(all P<0.05). Expression of miR-200b was much higher in PCOS patients with insulin resistance than in PCOS patients without insulin resistance and controls(P=0.012, P<0.001). In PCOS 山 东 大 学 学 报 (医 学 版)55卷1期 -贾月月,等.多囊卵巢综合征患者颗粒细胞microRNA-200b的表达及影响 \=-patients with insulin resistance, the index of homeostatic model assessment of insulin resistance(HOMA-IR)was positively correlated with miR-200b expression(r=0.532, P=0.002). Conclusion PCOS patients with insulin resistance have high morbidity of long-term metabolic complications. The high expression of miR-200b is positively related to insulin resistance, which may be the underlying pathogenic mechanism for insulin resistance in PCOS patients.

Key words: Granulosa cell, microRNA-200b, Insulin resistance, Polycystic ovary syndrome

CLC Number: 

  • R711.75
[1] Diamanti-Kandarakis E, Dunaif A. Insulin resistance and the polycystic ovary syndrome revisited: an update on mechanisms and implications[J]. Endocr Rev, 2012, 33(6): 981-1030.
[2] Shi Y, Zhao H, Shi Y, et al. Genome-wide association study identifies eight new risk loci for polycystic ovary syndrome[J]. Nat Genet, 2012, 44(9): 1020-1025.
[3] Chen ZJ, Zhao H, He L, et al. Genome-wide association study identifies susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21 and 9q33.3[J]. Nat Genet, 2011, 43(1): 55-59.
[4] Filios SR, Shalev A. β-cell microRNAs: small but powerful[J]. Diabetes, 2015, 64(11): 3631-3644.
[5] Jiang L, Huang J, Chen Y, et al. Identification of several circulating microRNAs from a genome-wide circulating microRNA expression profile as potential biomarkers for impaired glucose metabolism in polycystic ovarian syndrome[J]. Endocrine, 2016, 53(1): 280-290.
[6] Hyun S, Lee JH, Jin H, et al. Conserved MicroRNA miR-8/miR-200 and its target USH/FOG2 control growth by regulating PI3K[J]. Cell, 2009, 139(6): 1096-1108.
[7] Belgardt BF, Ahmed K, Spranger M, et al. The microRNA-200 family regulates pancreatic beta cell survival in type 2 diabetes[J]. Nat Med, 2015, 21(6): 619-627.
[8] 任红艳, 刘楠, 陶聪, 等. 小鼠体型控制相关microRNA-200b(miR-200b)的表达谱及靶标分析[J]. 农业生物技术学报, 2013, 1(21): 47-54. REN Hongyan, LIU Nan, TAO Cong, et al. The expression profile and target gene analysis of mouse(mus musculus)body size regulation related microRNA-200b(miR-200b)[J]. Journal of Agricultural Biotechnology, 2013, 1(21): 47-54.
[9] Dou L, Zhao T, Wang L, et al. miR-200s contribute to interleukin-6(IL-6)-induced insulin resistance in hepatocytes[J]. J Biol Chem, 2013, 288(31): 22596-22606.
[10] Kaur S, Archer KJ, Devi MG, et al. Differential gene expression in granulosa cells from polycystic ovary syndrome patients with and without insulin resistance: identification of susceptibility gene sets through network analysis[J]. J Clin Endocrinol Metab, 2012, 97(10): E2016-E2021.
[11] Group REAP. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome(PCOS)[J]. Hum Reprod, 2004, 19(1): 41-47.
[12] Matsubara H, Ikuta K, Ozaki Y, et al. Gonadotropins and cytokines affect luteal function through control of apoptosis in human luteinized granulosa cells[J]. J Clin Endocrinol Metab, 2000, 85(4): 1620-1626.
[13] Li MG, Ding GL, Chen XJ, et al. Association of serum and follicular fluid leptin concentrations with granulosa cell phosphorylated signal transducer and activator of transcription 3 expression in fertile patients with polycystic ovarian syndrome[J]. J Clin Endocrinol Metab, 2007, 92(12): 4771-4776.
[14] Dunaif A. Polycystic ovary syndrome in 2011: Genes, aging and sleep apnea in polycystic ovary syndrome[J]. Nat Rev Endocrinol, 2012, 8(2): 72-74.
[15] 黄佳, 倪仁敏, 陈晓莉, 等. 多囊卵巢综合征胰岛素抵抗及其与代谢综合征的相关性[C] // 中华医学会第十次全国妇产科学术会议妇科内分泌学组,厦门, 2012。
[16] Xu X, Zhao H, Shi Y, et al. Family association study between INSR gene polymorphisms and PCOS in Han Chinese[J]. Reprod Biol Endocrinol, 2011, 9: 76. doi: 10.1186/1477-7827-9-76.
[17] 张文娟, 吴兴国, 丁明德, 等. 脂联素基因多态性与多囊卵巢综合征发病关系的病例对照研究[J]. 中华妇产科杂志, 2015, 50(11): 825-829. ZHANG Wenjuan, WU Xingguo, DING Mingde, et al. Case-control based study between polymorphisms in the adiponectin gene and polycystic ovary syndrome[J]. Chinese Journal of Obstetrics and Gynecology, 2015, 50(11): 825-829.
[18] Sorensen AE, Wissing ML, Salo S, et al. MicroRNAs related to polycystic ovary syndrome(PCOS)[J]. Genes(Basel), 2014, 5(3): 684-708.
[19] Wu HL, Heneidi S, Chuang TY, et al. The expression of the miR-25/93/106b family of micro-RNAs in the adipose tissue of women with polycystic ovary syndrome[J]. J Clin Endocrinol Metab, 2014, 99(12): E2754-E2761.
[20] Park SM, Gaur AB, Lengyel E, et al. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2[J]. Genes Dev, 2008, 22(7): 894-907.
[21] Kim YK, Wee G, Park J, et al. TALEN-based knockout library for human microRNAs[J]. Nat Struct Mol Biol, 2013, 20(12): 1458-1464.
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