JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 69-74.doi: 10.6040/j.issn.1671-7554.0.2016.1313

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Expression and clinical significance of microRNA-183 in polycystic ovary syndrome with insulin resistance

LI Jingbo, LIU Hongbin, JIA Yueyue, WANG Ze, SUN Mei, SHI Yuhua   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China
  • Received:2016-10-14 Online:2017-01-10 Published:2017-01-10

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Abstract: Objective To explore the expression of microRNA-183(miR-183)in the ovarian granulosa cells of polycystic ovary syndrome(PCOS)patients with insulin resistance(IR), and to evaluate its clinical significance. Methods A total of 120 PCOS patients including 78 with insulin resistance and 42 without, and 72 healthy controls were enrolled. The miR-183 expression was examined by real-time quantitative reverse transcription PCR(qRT-PCR). The correlation between miR-183 and homeostasis model assessment-insulin resistance(HOMA-IR)was analyzed. The area under the receiver operating characteristic(ROC)curve(AUC)was calculated to evaluate the predictive power of miR-183 for PCOS insulin resistance. Results PCOS patients with insulin resistance had higher miR-183 expression than the control group(P<0.001)and PCOS without insulin resistance group(P=0.034). There was positive correlation 山 东 大 学 学 报 (医 学 版)55卷1期 -李婧博,等.microRNA-183在PCOS胰岛素抵抗中的表达及其临床意义 \=-between HOMA-IR and miR-183 expression in PCOS with insulin resistance group(r=0.476, P=0.003). The ROC analysis suggested that combination of miR-183 and body mass index(BMI)(AUC: 0.820, 95%CI: 0.750-0.889, P<0.001)might be an effective indicator. Conclusion The high expression of miR-183 is correlated to insulin resistance of PCOS and HOMA-IR, and may be involved in the pathogenesis of PCOS insulin resistance through follicular microenvironment.

Key words: MicroRNA-183, Insulin resistance, Granulosa cell, Polycystic ovary syndrome

CLC Number: 

  • R711.75
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