JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 88-93.doi: 10.6040/j.issn.1671-7554.0.2016.929

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Screening key genes for TXNDC5 and insulin signaling pathway

LI Jian1,2, XU Bing2, YAN Xinfeng3, XU Wanju4, CHANG Xiaotian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Medical Research Center;
    3. Department of Orthopedics;
    4. Clinical Laboratory, Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China
  • Received:2016-08-01 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To explore the pathogenic mechanism of thioredoxin domain containing protein 5(TXNDC5)involvement in rheumatoid arthritis(RA). Methods Human rheumatoid arthritis synovial cells(RASFs)were separated from RA patients. After the TXNDC5 expression was inhibited by anti-TXNDC5 siRNA, the total RNA was pooled, and the expressions of related genes were detected with PCR Array. The mRNA and protein expressions of related genes were verified using RT-PCR and ELISA. Results PCR Array analyses demonstrated that IGF-1, PCK1, SLC2A4, IL1R and IGFBP1 expressions significantly changed after TXNDC5 expression was inhibited. RT-PCR and ELISA verification indicated that IGFBP1 expression was significantly enhanced in both mRNA and protein levels(both P<0.001), while expressions of IGF-1, PCK1, SLC2A4 and IL1R1 showed no significant difference(P=0.792 7, P=0.713 0, P=0.452 9, P=0.354 2). No difference was observed in IGFBP3 expressions(P=0.511 5). Conclusion In RASFs, TXNDC5 is involved in RA by regulating the expression of IGFBP1.

Key words: Insulin-like growth factor binding protein 1, Rheumatoid arthritis, Thioredoxin domain containing protein 5, Insulin resistance, Insulin signal pathway

CLC Number: 

  • R34
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